{"id":17588,"date":"2020-10-05T11:09:23","date_gmt":"2020-10-05T02:09:23","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=17588"},"modified":"2021-01-29T14:27:14","modified_gmt":"2021-01-29T05:27:14","slug":"oct_2020","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/current_tech\/oct_2020","title":{"rendered":"BRC Current Technology October 2020"},"content":{"rendered":"<table style=\"max-width:700px; border:0px;\">\n<tbody>\n<tr>\n<td style=\"border:0px;background-color:#fff; text-align: center; white-space: nowrap; position:absolute;\"><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/2013\/05\/brc_logo2.png\" alt=\"RIKEN BRC\" \/><\/td>\n<td style=\"border:0px;background-color:#fff; text-align: center;\"><a href=\"\/ja\/current_tech\" style=\"text-decoration: none;\"><span style=\"color:#000000; font-size: 25pt; line-height: 130%; font-weight: bold;\">BRC Current Technology<\/span><br \/>\n<span style=\"color:#0000ff; font-size: 25pt; line-height: 130%; font-weight: bold;\">October 2020<\/span><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border:0px;background-color:#fff; text-align: center;\" colspan=\"2\">\n<h1 style=\"color: #0000ff;\">14. 1\u4e16\u4ee3\u3067ES\u7d30\u80de\u7531\u6765\u306e\u7cbe\u5b50\u306e\u307f\u3092\u6301\u3064<br \/>\u30ad\u30e1\u30e9\u30de\u30a6\u30b9\u3092\u4f5c\u51fa<\/h1>\n<h2>\u30b3\u30f3\u30c7\u30a3\u30b7\u30e7\u30ca\u30eb\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u3092\u7528\u3044\u306a\u3044<br \/>\u81f4\u6b7b\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u89e3\u6790<\/h2>\n<p><a href=\"\/ja\/wp-content\/uploads\/2020\/10\/Current tech_Fig_202010_jp.jpg\"><img decoding=\"async\" alt=\"\u56f3\u89e3\u30ad\u30e1\u30e9\u30de\u30a6\u30b9\u4f5c\u51fa\u65b9\u6cd5\" src=\"\/ja\/wp-content\/uploads\/2020\/10\/Current tech_Fig_202010_jp.jpg\" style=\"text-align:center;\" width=\"650\" height=\"\" \/><\/a>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"border:0px;background-color:#fff; white-space: normal; text-indent: 1em;\" colspan=\"2\">RIKEN BRC \u3067\u306f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3092\u5229\u7528\u3057\u305f\u767a\u751f\u5de5\u5b66\u7814\u7a76\u958b\u767a\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002\u7d44\u7e54\u7279\u7570\u7684\u3042\u308b\u3044\u306f\u6642\u671f\u7279\u7570\u7684\u306b\u907a\u4f1d\u5b50\u3092\u6b20\u640d\u3055\u305b\u305f\u30b3\u30f3\u30c7\u30a3\u30b7\u30e7\u30ca\u30eb\u30ce\u30c3\u30af\u30a2\u30a6\u30c8 (cKO) \u30de\u30a6\u30b9\u306f\u3001\u5168\u8eab\u3067\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u3059\u308b\u3068\u81f4\u6b7b\u3068\u306a\u308b\u907a\u4f1d\u5b50 (\u81f4\u6b7b\u907a\u4f1d\u5b50) \u306e\u6a5f\u80fd\u89e3\u6790\u306b\u3042\u305f\u308a\u5f37\u529b\u306a\u30c4\u30fc\u30eb\u3067\u3059\u304c\u3001cKO\u30de\u30a6\u30b9\u306e\u4f5c\u51fa\u306b\u306f\u4e00\u822c\u306b\u6642\u9593\u3068\u52b4\u529b\u3092\u8981\u3057\u307e\u3059\u3002\u305d\u3053\u3067\u6211\u3005\u306f\u3001\u76ee\u7684\u306e\u7d44\u7e54\u304c\u907a\u4f1d\u5b50\u5909\u7570\u3092\u6709\u3059\u308bES\u7d30\u80de\u306e\u307f\u306b\u7531\u6765\u3059\u308b\u30ad\u30e1\u30e9\u30de\u30a6\u30b9\u3092\u7c21\u4fbf\u306b\u4f5c\u51fa\u3059\u308b\u65b9\u6cd5\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002\u307e\u305a\u3001\u751f\u6b96\u7d30\u80de\u306e\u767a\u751f\u306b\u5fc5\u9808\u306e<i>Nanos3<\/i>\u907a\u4f1d\u5b50\u306e\u30a8\u30af\u30bd\u30f3\u3092\u6a19\u7684\u3068\u3057\u305f3\u7a2e\u985e\u306eguide RNA (sg<i>Nanos3<\/i>) \u3068Cas9 mRNA\u3092\u91ce\u751f\u578b\u30de\u30a6\u30b9\u53d7\u7cbe\u5375\u306b\u9855\u5fae\u6ce8\u5165\u3057\u3001\u30db\u30b9\u30c8\u306e\u80da\u76e4\u80de\u3092\u4f5c\u6210\u3057\u307e\u3059\u3002\u305d\u3053\u3078\u81f4\u6b7b\u907a\u4f1d\u5b50\u3067\u3042\u308b<i>Dnmt3b<\/i>\u3092\u6b20\u640d\u3057\u305f\u96c4ES\u7d30\u80de\u3092\u6ce8\u5165\u3057\u307e\u3057\u305f\u3002\u4f5c\u51fa\u3057\u305f\u96c4\u306e\u30ad\u30e1\u30e9\u30de\u30a6\u30b9\u304b\u3089\u751f\u307e\u308c\u305f\u7523\u5b50\u306f\u5168\u3066ES\u7d30\u80de\u306b\u7531\u6765\u3057\u3066\u304a\u308a\u3001\u30ad\u30e1\u30e9\u30de\u30a6\u30b9\u304cES\u7d30\u80de\u306b\u7531\u6765\u3059\u308b\u7cbe\u5b50\u306e\u307f\u3092\u4f5c\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u904e\u53bb\u306ecKO\u30de\u30a6\u30b9\u3092\u7528\u3044\u305f\u5831\u544a\u3068\u540c\u69d8\u306b\u3001<i>Dnmt3b<\/i>\u304c\u96c4\u306e\u751f\u6b96\u7d30\u80de\u306e\u5206\u5316\u306b\u5fc5\u9808\u3067\u306f\u306a\u3044\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002\u4eca\u5f8c\u3053\u306e\u624b\u6cd5\u304c\u3001\u4ed6\u306e\u7d44\u7e54\u306b\u304a\u3051\u308b\u81f4\u6b7b\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u89e3\u6790\u3078\u5fdc\u7528\u3055\u308c\u308b\u3053\u3068\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<table style=\"max-width:700px;border: 0px;\">\n<tbody>\n<tr>\n<td style=\"border:0px;background-color:#fff;\" rowspan=\"2\" valign=\"top\">References:<\/td>\n<td style=\"border:0px;background-color:#fff;\" valign=\"top\">[1]<\/td>\n<td style=\"border:0px;background-color:#fff;\" valign=\"top\">K Miura et al. \u201cGeneration of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for <i>Nanos3<\/i>\u201d<br \/>\n<a href=\"https:\/\/doi.org\/10.1093\/biolre\/ioaa176\" target=\"_blank\" rel=\"noopener noreferrer\"><em>Biol Reprod<\/em>, DOI: 10.1093\/biolre\/ioaa176<\/a><\/td>\n<\/tr>\n<td style=\"border:0px;background-color:#fff;\" valign=\"top\">[2]<\/td>\n<td style=\"border:0px;background-color:#fff;\" valign=\"top\">JC Schimenti. \u201cConditional surrender in one generation: determining the reproductive roles of mouse embryo lethal genes by embryo complementation\u201d<br \/>\n<a href=\"https:\/\/doi.org\/10.1093\/biolre\/ioaa193\" target=\"_blank\" rel=\"noopener noreferrer\"><em>Biol Reprod<\/em>, DOI: 10.1093\/biolre\/ioaa193<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>BRC Current Technology October 2020 14. 1\u4e16\u4ee3\u3067ES\u7d30\u80de\u7531\u6765\u306e\u7cbe\u5b50\u306e\u307f\u3092\u6301\u3064\u30ad\u30e1\u30e9\u30de\u30a6\u30b9\u3092\u4f5c\u51fa \u30b3\u30f3\u30c7\u30a3\u30b7\u30e7\u30ca\u30eb\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u3092\u7528\u3044\u306a\u3044\u81f4\u6b7b\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u89e3\u6790 RIKEN BRC \u3067\u306f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3092\u5229\u7528\u3057\u305f\u767a\u751f\u5de5\u5b66\u7814\u7a76\u958b\u767a\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002\u7d44\u7e54\u7279\u7570\u7684\u3042\u308b\u3044\u306f\u6642\u671f\u7279\u7570\u7684\u306b\u907a\u4f1d\u5b50\u3092\u6b20\u640d\u3055\u305b\u305f\u30b3\u30f3\u30c7\u30a3\u30b7\u30e7\u30ca\u30eb\u30ce\u30c3\u30af\u30a2\u30a6\u30c8 (cKO) \u30de\u30a6\u30b9\u306f\u3001\u5168\u8eab\u3067\u30ce\u30c3\u30af\u30a2\u30a6 [&hellip;]<\/p>\n","protected":false},"author":19,"featured_media":0,"parent":3268,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-17588","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/17588","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=17588"}],"version-history":[{"count":8,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/17588\/revisions"}],"predecessor-version":[{"id":17912,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/17588\/revisions\/17912"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3268"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=17588"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}