{"id":17712,"date":"2020-11-30T13:57:00","date_gmt":"2020-11-30T04:57:00","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=17712"},"modified":"2021-04-07T13:36:50","modified_gmt":"2021-04-07T04:36:50","slug":"dec_2020_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/dec_2020_mm","title":{"rendered":"\u5149\u5909\u63db\u306b\u3088\u308a\u7d30\u80de\u52d5\u614b\u3092\u30e2\u30cb\u30bf\u30fc\u53ef\u80fd\u306aKaede\u30de\u30a6\u30b9"},"content":{"rendered":"\n\n\n
\"RIKEN<\/td>\nDecember 2020<\/span>
\nMouse of the Month<\/span><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n
\n

\u5149\u5909\u63db\u306b\u3088\u308a\u7d30\u80de\u52d5\u614b\u3092\u30e2\u30cb\u30bf\u30fc\u53ef\u80fd\u306aKaede\u30de\u30a6\u30b9<\/span><\/p>\n

\nB6.Cg-Tg(CAG-tdKaede)15Utr (RBRC05737)<\/a>
\nB6.Cg-c\/c Tg(CAG-tdKaede)15Utr (RBRC05738)<\/a>
\nC.Cg-Tg(CAG-tdKaede)15Utr (RBRC09257)<\/a>
\n<\/h5>\n

\n\"Photoconversion\"<\/a>\n<\/p>\n

Courtesy of Yoshihiro Miwa, Ph.D.<\/p>\n

Photoconversion can be induced in both limited spot aria (left : for target cells)
and wide aria (right : for target organs) by choosing illuminant of violet light.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
\n

Kaede\u30de\u30a6\u30b9\u306f\u3001CAG\u30d7\u30ed\u30e2\u30fc\u30bf\u5236\u5fa1\u4e0b\u3067\u30a4\u30b7\u30b5\u30f3\u30b4\u306e1\u7a2e\u306b\u7531\u6765\u3059\u308b\u5149\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u300cKaede\u300d\u3092\u767a\u73fe\u3059\u308b\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30de\u30a6\u30b9\u3067\u3059[1,2]\u3002\u901a\u5e38\u72b6\u614b\u3067\u306f\u3001\u5168\u8eab\u3067\u3001\u6052\u5e38\u7684\u306b\u7dd1\u8272\u86cd\u5149\u3092\u767a\u3057\u3001\u7d2b\u5149\u3092\u5f53\u3066\u308b\u3068\u3001\u7167\u5c04\u90e8\u5206\u306e\u307f\u304c\u8d64\u8272\u86cd\u5149\u3078\u3068\u5149\u5909\u63db(photoconversion)\u3092\u8d77\u3053\u3059\u306e\u304c\u7279\u5fb4\u3067\u3059\u3002\u3053\u306e\u5149\u5909\u63db\u306f\u3001\u7d30\u80de\u6a5f\u80fd\u305d\u306e\u3082\u306e\u306b\u306f\u5f71\u97ff\u3092\u4e0e\u3048\u305a\u3001\u307e\u305f\u3001\u5206\u88c2\u56de\u6570\u304c\u5c11\u306a\u3044\u7d30\u80de\u3067\u306f\u3001\u6bd4\u8f03\u7684\u9577\u3044\u671f\u9593\u3001\u8d64\u8272\u86cd\u5149\u3092\u89b3\u5bdf\u3067\u304d\u308b\u3053\u3068\u304b\u3089\u3001\u7279\u5b9a\u306e\u7d30\u80de\u3092\u30e9\u30d9\u30eb\u3057\u3001\u305d\u306e\u52d5\u614b\u3092\u53ef\u8996\u5316\u3059\u308b\u969b\u306b\u6709\u7528\u306a\u7cfb\u7d71\u3068\u3057\u3066\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u5b9f\u969b\u306b\u672c\u7cfb\u7d71\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u6642[3]\u3084\u75be\u60a3\u767a\u75c7\u6642[4]\u3001\u816b\u760d\u6d78\u6f64\u6642[5]\u3068\u3044\u3063\u305f\u74b0\u5883\u6761\u4ef6\u306e\u4e2d\u3067\u3001\u5404\u7a2e\u514d\u75ab\u7d30\u80de\u304c\u3001\u7d44\u7e54\u3084\u81d3\u5668\u306e\u9593\u3092\u3069\u306e\u3088\u3046\u306b\u52d5\u304f\u306e\u304bin vivo<\/i>\u3067\u30e2\u30cb\u30bf\u30fc\u3059\u308b\u305f\u3081\u306b\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u305d\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u65b9\u6cd5\u3082\u591a\u5c90\u306b\u308f\u305f\u308a\u3001Kaede\u30de\u30a6\u30b9\u81ea\u8eab\u306e\u89e3\u6790\u306b\u9650\u3089\u305a\u3001\u4ed6\u306e\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30a6\u30b9\u3068\u4ea4\u914d\u3057\u305f\u4f8b\u3084\u3001\u9aa8\u9ac4\u30ad\u30e1\u30e9\u30de\u30a6\u30b9\u3092\u4f5c\u88fd\u3057\u305f\u4f8b\u306a\u3069\u3082\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4eca\u56de\u3001\u5bc4\u8a17\u8005\u306e\u4e09\u8f2a\u4f73\u5b8f\u5148\u751f\u3088\u308a\u3001Kaede\u30de\u30a6\u30b9\u306e\u5149\u7167\u5c04\u65b9\u6cd5\u306b\u95a2\u3059\u308b\u8cc7\u6599\u3092\u3054\u63d0\u4f9b\u3044\u305f\u3060\u304d\u307e\u3057\u305f[6]\u3002Kaede\u30de\u30a6\u30b9\u306f\u73fe\u5728\u306e\u3068\u3053\u308d\u3001\u7279\u306b\u514d\u75ab\u5b66\u5206\u91ce\u3067\u5229\u7528\u5b9f\u7e3e\u306e\u9ad8\u3044\u7cfb\u7d71\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u5149\u5909\u63db\u306b\u3088\u308b\u7d30\u80de\u52d5\u614b\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u306f\u3001\u69d8\u3005\u306a\u7814\u7a76\u5206\u91ce\u3067\u6709\u7528\u306a\u624b\u6cd5\u3067\u3042\u308a\u3001\u4eca\u5f8c\u306e\u5fdc\u7528\u5229\u7528\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Depositor<\/td>\n:<\/td>\n\u4e09\u8f2a\u3000\u4f73\u5b8f \u5148\u751f
\u7b51\u6ce2\u5927\u5b66 (\u5bc4\u8a17\u5f53\u6642)\n<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nB6.Cg-Tg(CAG-tdKaede)15Utr
(C57BL\/6 background)<\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC05737<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nB6.Cg-c\/c Tg(CAG-tdKaede)15Utr
(\u30a2\u30eb\u30d3\u30ceC57BL\/6 background)\n<\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC05738<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nC.Cg-Tg(CAG-tdKaede)15Utr
(BALB\/c background)\n<\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC09257<\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\n[1]<\/td>\nTomura M, Yoshida N, Tanaka J, Karasawa S, Miwa Y, Miyawaki A, Kanagawa O.
\nMonitoring cellular movement in vivo with photoconvertible fluorescence protein “Kaede” transgenic mice.
\nProc Natl Acad Sci U S A.<\/em> 2008 Aug 5;105(31):10871-6.
\n<\/a><\/td>\n<\/tr>\n
[2]<\/td>\nMonitoring cell replacement and migration in vivo<\/i> with Kaede.
\nMouse of the Month August 2016<\/a><\/td>\n<\/tr>\n
[3]<\/td>\nBeura LK, Wijeyesinghe S, Thompson EA, Macchietto MG, Rosato PC, Pierson MJ, Schenkel JM, Mitchell JS, Vezys V, Fife BT, Shen S, Masopust D.
\nT Cells in Nonlymphoid Tissues Give Rise to Lymph-Node-Resident Memory T Cells.
\nImmunity.<\/em>2018 Feb 20;48(2):327-338.e5.<\/a><\/td>\n<\/tr>\n
[4]<\/td>\nCalcinotto A, Brevi A, Chesi M, Ferrarese R, Garcia Perez L, Grioni M, Kumar S, Garbitt VM, Sharik ME, Henderson KJ, Tonon G, Tomura M, Miwa Y, Esplugues E, Flavell RA, Huber S, Canducci F, Rajkumar VS, Bergsagel PL, Bellone M.
\nMicrobiota-driven interleukin-17-producing cells and eosinophils synergize to accelerate multiple myeloma progression.
\nNat Commun.<\/em> 2018 Dec 3;9(1):4832.<\/a><\/td>\n<\/tr>\n
[5]<\/td>\nTorcellan T, Hampton HR, Bailey J, Tomura M, Brink R, Chtanova T.
\nIn vivo photolabeling of tumor-infiltrating cells reveals highly regulated egress of T-cell subsets from tumors.
\nProc Natl Acad Sci U S A.<\/em> 2017 May 30;114(22):5677-5682.<\/a><\/td>\n<\/tr>\n
[6]<\/td>\nKaede\u30de\u30a6\u30b9\u3078\u306e\u7167\u5c04\u65b9\u6cd5
\n\"PDFPDF<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
December 2020
\nSaori Mizuno, Ph.D.
\nContact: Experimental Animal Division, RIKEN BioResource Research Center (animal.brc@riken.jp)<\/a>
\nAll materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

December 2020 Mouse of the Month \u5149\u5909\u63db\u306b\u3088\u308a\u7d30\u80de\u52d5\u614b\u3092\u30e2\u30cb\u30bf\u30fc\u53ef\u80fd\u306aKaede\u30de\u30a6\u30b9 B6.Cg-Tg(CAG-tdKaede)15Utr (RBRC05737) B6.Cg-c\/c Tg(CAG-tdKaede)15Utr (RBRC05738) C.Cg-Tg(CAG-tdKaede)15Utr (RBRC09257) Courtesy of Yoshihir […]<\/p>\n","protected":false},"author":19,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-17712","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/17712","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=17712"}],"version-history":[{"count":8,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/17712\/revisions"}],"predecessor-version":[{"id":18744,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/17712\/revisions\/18744"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=17712"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}