{"id":18403,"date":"2021-08-03T10:20:23","date_gmt":"2021-08-03T01:20:23","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=18403"},"modified":"2022-06-13T10:30:51","modified_gmt":"2022-06-13T01:30:51","slug":"aug_2021","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/current_tech\/aug_2021","title":{"rendered":"BRC Current Technology August 2021"},"content":{"rendered":"\n\n\n\n\n\n\n\n\n\n\n\n \n
\"RIKEN<\/td>\nBRC Current Technology<\/span>
\nAugust 2021<\/span><\/a><\/td>\n<\/tr>\n
\n

17. CRISPR\/Cas9\u3067\u9ad8\u52b9\u7387\u306b
\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u30de\u30a6\u30b9\u3092\u4f5c\u88fd<\/h1>\n

\u8907\u6570\u907a\u4f1d\u5b50\u306e\u540c\u6642\u7834\u58ca\u3082\u53ef\u80fd<\/h2>\n<\/tr>\n

\"\u56f31\"<\/a><\/td>\n<\/tr>\n
\u56f31. CRISPR\u306b\u3088\u308b\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\uff08KO\uff09\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u30de\u30a6\u30b9\u306e\u4f5c\u88fd\u6cd5\u3002<\/td>\n<\/tr>\n
(a)<\/td>\n\u30bf\u30f3\u30d1\u30af\u3092\u30b3\u30fc\u30c9\u3059\u308b\u30a8\u30af\u30bd\u30f3\u306e\u7570\u306a\u308b2\u304b\u6240\u4ee5\u4e0a\u306b\u5bfe\u3057\u3066guide RNA\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u3002<\/td>\n<\/tr>\n
(b)<\/td>\n\u4e0a\u8a18\u306e\u3088\u3046\u306b\u3057\u3066\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u8907\u6570\u306eguide RNA\u3092Cas9<\/i> mRNA\u3068\u540c\u6642\u306b\u53d7\u7cbe\u5375\u306b\u6ce8\u5165\u3059\u308b\u3068\u3001
\u975e\u5e38\u306b\u9ad8\u3044\u52b9\u7387\uff0895-100%\uff09\u3067\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u3092\u7834\u58ca\u3067\u304d\u308b\u3002<\/td>\n<\/tr>\n
\"\u56f32\"<\/a><\/td>\n<\/tr>\n
\u56f32. Tuba3a<\/i>\u3068Tuba3b<\/i>\u907a\u4f1d\u5b50\u306e\u540c\u6642\u7834\u58ca\u306b\u3088\u3063\u3066\u8d77\u3053\u308b\u751f\u6b96\u7d30\u80de\u767a\u751f\u7570\u5e38\u3002<\/td>\n<\/tr>\n
Tuba3a<\/i>\u3001Tuba3b<\/i>\u305d\u308c\u305e\u308c\u5358\u72ecKO\u3057\u305f\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u30de\u30a6\u30b9\u306f\u7279\u306b\u7570\u5e38\u3092\u793a\u3055\u306a\u3044\u304c\u30012\u907a\u4f1d\u5b50\u3092\u540c\u6642\u306b\u7834\u58ca\u3057\u305f\u30c0\u30d6\u30ebKO\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u30de\u30a6\u30b9\u3067\u306f\u7cbe\u5de3\u304c\u9855\u8457\u306b\u59d4\u7e2e\u3057\u3066\u304a\u308a\u3001DDX4\u967d\u6027\u306e\u751f\u6b96\u7d30\u80de\u304c\u5b8c\u5168\u306b\u6d88\u5931\u3057\u3066\u3044\u305f\u3002<\/td>\n<\/tr>\n
\u3053\u308c\u307e\u3067\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u306e\u8868\u73fe\u578b\u89e3\u6790\u30a2\u30d7\u30ed\u30fc\u30c1\u3067\u306f\u3001\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u30de\u30a6\u30b9\u3092\u4ea4\u914d\u306b\u3088\u308a\u7cfb\u7d71\u5316\u3057\u305f\u3046\u3048\u3067\u3001\u30d8\u30c6\u30ed\u500b\u4f53\u540c\u58eb\u306e\u4ea4\u914d\u3092\u4ecb\u3057\u3066\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u3092\u4f5c\u88fd\u3057\u3066\u304d\u307e\u3057\u305f\u3002\u3053\u306e\u3088\u3046\u306a\u30a2\u30d7\u30ed\u30fc\u30c1\u3067\u306f\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u4f5c\u88fd\u306e\u5f8c\u3082\u6570\u4e16\u4ee3\u306e\u4ea4\u914d\u3092\u5fc5\u8981\u3068\u3059\u308b\u305f\u3081\u3001\u89e3\u6790\u306b\u975e\u5e38\u306b\u9577\u3044\u6642\u9593\u304c\u304b\u304b\u3063\u3066\u3044\u307e\u3057\u305f\u3002CRISPR\/Cas9\u6cd5\u306f\u9ad8\u52b9\u7387\u306b\u30bf\u30fc\u30b2\u30c3\u30c8\u9818\u57df\u306e\u30b2\u30ce\u30e0\u3092\u7de8\u96c6\u3067\u304d\u308b\u9769\u65b0\u7684\u306a\u6280\u8853\u3067\u3059\u3002\u3053\u308c\u307e\u3067\u3001CRISPR\/Cas9\u306eguide RNA\u30921\u907a\u4f1d\u5b50\u306b\u5bfe\u3057\u3066\u8907\u6570\u540c\u6642\u306b\u6ce8\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u9ad8\u3044\u52b9\u7387\u3067\u4e21\u30a2\u30ec\u30eb\u306b\u5909\u7570\u306e\u5165\u3063\u305f\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u30de\u30a6\u30b9\u3092\u4f5c\u88fd\u3067\u304d\u308b\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059[1]\u3002\u6211\u3005\u3082\u3053\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u306b\u3088\u3063\u3066\u3001\u307b\u307c100%\u306e\u52b9\u7387\u3067\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u30de\u30a6\u30b9\u3092\u4f5c\u88fd\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u3066\u304d\u307e\u3057\u305f[2]\u3002\u4eca\u56de\u3001\u3053\u306e\u624b\u6cd5\u30922\u907a\u4f1d\u5b50\u306b\u5bfe\u3057\u3066\u540c\u6642\u306b\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u30012\u907a\u4f1d\u5b50\u306e\u30c0\u30d6\u30ebKO\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u30de\u30a6\u30b9\u3092\u76f4\u63a5\u4f5c\u88fd\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u307e\u3057\u305f[3]\u3002Tuba3a<\/i>\u3068Tuba3b<\/i>\u907a\u4f1d\u5b50\u3092\u540c\u6642\u7834\u58ca\u3057\u305f\u7d50\u679c\u3001\u7cbe\u5de3\u306e\u751f\u6b96\u7d30\u80de\u304c\u5b8c\u5168\u306b\u6d88\u5931\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u3088\u3046\u306a\u8907\u6570\u907a\u4f1d\u5b50\u306e\u540c\u6642\u7834\u58ca\u306f\u3001\u3053\u308c\u307e\u3067\u306e\u4ea4\u914d\u3092\u30d9\u30fc\u30b9\u3068\u3057\u305f\u30a2\u30d7\u30ed\u30fc\u30c1\u3068\u6bd4\u3079\u3066\u683c\u6bb5\u306b\u6642\u9593\u3092\u77ed\u7e2e\u3067\u304d\u308b\u305f\u3081\u3001\u8868\u73fe\u578b\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u6cd5\u3068\u3057\u3066\u975e\u5e38\u306b\u6709\u7528\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n
References:<\/td>\n[1]<\/td>\nE. A. Susaki et al.<\/i>, \u201cNext-generation mammalian genetics toward organism-level systems biology,\u201d
\nnpj Sys. Biol. Appl.<\/i>, 3, 1\u201310, 2017.<\/a><\/td>\n<\/tr>\n
[2]<\/td>\nS. Matoba et al.<\/i>, \u201cPaternal knockout of Slc38a4\/SNAT4 causes placental hypoplasia associated with intrauterine growth restriction in mice,\u201d
\nProc. Natl. Acad. Sci. U. S. A.<\/i>, 116, 21047\u201321053, 2019.<\/a><\/td>\n<\/tr>\n
[3]<\/td>\nM. S. Akter et al.<\/i>, \u201cCRISPR\/Cas9-based genetic screen of SCNT-reprogramming resistant genes identifies critical genes for male germ cell development in mice,\u201d
\nSci. Rep.<\/i>, DOI: 10.1038\/s41598-021-94851-9, 2021.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

BRC Current Technology August 2021 17. CRISPR\/Cas9\u3067\u9ad8\u52b9\u7387\u306b\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u30de\u30a6\u30b9\u3092\u4f5c\u88fd \u8907\u6570\u907a\u4f1d\u5b50\u306e\u540c\u6642\u7834\u58ca\u3082\u53ef\u80fd \u56f31. CRISPR\u306b\u3088\u308b\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\uff08KO\uff09\u30d5\u30a1\u30a6\u30f3\u30c0\u30fc\u30de\u30a6\u30b9\u306e\u4f5c\u88fd\u6cd5\u3002 (a) \u30bf\u30f3\u30d1\u30af\u3092\u30b3\u30fc\u30c9\u3059\u308b\u30a8\u30af\u30bd\u30f3\u306e\u7570\u306a\u308b2\u304b\u6240\u4ee5\u4e0a\u306b\u5bfe\u3057\u3066guide RNA\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u3002 (b) \u4e0a\u8a18\u306e\u3088\u3046\u306b\u3057\u3066\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u8907\u6570\u306eguid […]<\/p>\n","protected":false},"author":19,"featured_media":0,"parent":3268,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-18403","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/18403","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=18403"}],"version-history":[{"count":3,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/18403\/revisions"}],"predecessor-version":[{"id":19030,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/18403\/revisions\/19030"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3268"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=18403"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}