{"id":18839,"date":"2022-01-31T10:37:45","date_gmt":"2022-01-31T01:37:45","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=18839"},"modified":"2022-10-25T10:44:10","modified_gmt":"2022-10-25T01:44:10","slug":"feb_2022_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/feb_2022_mm","title":{"rendered":"\u65b0\u898f\u30bf\u30f3\u30d1\u30af\u8cea\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u6280\u8853 \u30aa\u30fc\u30ad\u30b7\u30f3\u30c7\u30b0\u30ed\u30f3(AID) 2\u6cd5"},"content":{"rendered":"\n\n\n
\"RIKEN<\/td>\nFebruary 2022<\/span>
\nMouse of the Month<\/span><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n
\n

\u65b0\u898f\u30bf\u30f3\u30d1\u30af\u8cea\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u6280\u8853
\u30aa\u30fc\u30ad\u30b7\u30f3\u30c7\u30b0\u30ed\u30f3(AID) 2\u6cd5<\/span><\/p>\n

\nB6;B6D2-Tg(CAG-OsTIR1-P2A-mAID-EGFP-nanoluc)#16Ysa (RBRC11438)<\/a>
\nB6;B6D2-Tg(CAG-OsTIR1-P2A-mAID-EGFP-nanoluc)#19Ysa (RBRC11439)<\/a>
\n<\/h5>\n

\n\"\u30b7\u30b0\u30ca\u30eb\u6bd4\u8f03\"<\/a><\/p>\n

Courtesy of Yumiko Saga, Ph.D.<\/p>\n

\u4eca\u56de\u958b\u767a\u3057\u305f\u86cb\u767d\u5206\u89e3\u7cfb\u30de\u30a6\u30b9(RBRC11438)\u3068\u4ea4\u914d\u3057\u305f\u598a\u5a20\u30de\u30a6\u30b9\u306b\u3001\u30aa\u30fc\u30ad\u30b7\u30f3\u30a2\u30ca\u30ed\u30b0(5-Ph-IAA)\u3092\u6295\u4e0e\u3059\u308b\u3068\u3001\u80ce\u4ed4\u306e\u30ec\u30dd\u30fc\u30bf\u30fcEGFP\u306f\u901f\u3084\u304b\u306b\u5206\u89e3\u3055\u308c\u30011\u6642\u9593\u5f8c\u306b\u306f\u30b7\u30b0\u30ca\u30eb\u304c\u6d88\u5931\u3059\u308b\u3002\n<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n
\u30bf\u30f3\u30d1\u30af\u8cea\u6a5f\u80fd\u3092\u63a2\u7d22\u3059\u308b\u624b\u6bb5\u3068\u3057\u3066\u3001\u7d30\u80de\u5185\u30bf\u30f3\u30d1\u30af\u8cea\u91cf\u3092\u8abf\u7bc0\u3057\u3001\u89e3\u6790\u3059\u308b\u624b\u6cd5\u306f\u6709\u7528\u3067\u3059\u3002\u5f93\u6765\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u5236\u5fa1\u3059\u308b\u3001\u907a\u4f1d\u5b50\u30bf\u30fc\u30b2\u30c6\u30a3\u30f3\u30b0\u6cd5\u3084RNAi\u6cd5\u306a\u3069\u304c\u3088\u304f\u7528\u3044\u3089\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u6700\u8fd1\u3001\u3088\u308a\u8fc5\u901f\u306b\u3001\u3088\u308a\u76f4\u63a5\u7684\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u5236\u5fa1\u3092\u884c\u3046\u3001\u30c7\u30b0\u30ed\u30f3\u6280\u8853\u304c\u6ce8\u76ee\u3092\u96c6\u3081\u3066\u3044\u307e\u3059\u3002\u30c7\u30b0\u30ed\u30f3\u6280\u8853\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u3092\u8a98\u5c0e\u3059\u308b\u30da\u30d7\u30c1\u30c9\u914d\u5217(\u30c7\u30b0\u30ed\u30f3\u914d\u5217)\u3092\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u4ed8\u52a0\u3057\u3001\u30ea\u30ac\u30f3\u30c9\u3092\u4ecb\u3057\u3066\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u30fb\u9664\u53bb\u3092\u8a98\u5c0e\u3059\u308b\u624b\u6cd5\u3067\u3059\u3002\u4eca\u56de\u3054\u7d39\u4ecb\u3059\u308b\u30aa\u30fc\u30ad\u30b7\u30f3\u30c7\u30b0\u30ed\u30f3(AID)2\u6cd5[1]\u306f\u30012009\u5e74\u306b\u5148\u884c\u3057\u3066\u767a\u8868\u3055\u308c\u305fAID\u6cd5[2,3]\u306e\u6539\u826f\u7248\u3067\u3001\u7d30\u80de\u5185\u306e\u30e6\u30d3\u30ad\u30c1\u30f3\u30fb\u30d7\u30ed\u30c6\u30a2\u30bd\u30fc\u30e0\u7d4c\u8def\u3068\u690d\u7269\u306e\u30aa\u30fc\u30ad\u30b7\u30f3\u5206\u89e3\u7d4c\u8def\u3092\u5229\u7528\u3057\u3066\u958b\u767a\u3055\u308c\u307e\u3057\u305f\u3002\u30aa\u30fc\u30ad\u30b7\u30f3\u7d50\u5408\u90e8\u4f4d\u306b\u5909\u7570\u3092\u5c0e\u5165\u3057\u305fTIR1(F74G)\u3068\u3001\u30aa\u30fc\u30ad\u30b7\u30f3\u8a98\u5c0e\u6027\u30c7\u30b0\u30ed\u30f3\u30bf\u30b0AID\u3001\u30ea\u30ac\u30f3\u30c9\u3068\u306a\u308b\u30aa\u30fc\u30ad\u30b7\u30f3\u985e\u4f3c\u5316\u5408\u72695-Ph-IAA\u30013\u3064\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u3088\u308a\u3001\u7279\u5b9a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u89e3\u3092\u5b9f\u73fe\u3057\u307e\u3059\u3002AID2\u6cd5\u306f\u3001\u5f93\u6765\u306eAID\u6cd5\u3068\u6bd4\u8f03\u3057\u3066\u3001\u30ea\u30ac\u30f3\u30c9\u975e\u6dfb\u52a0\u6642\u306e\u30ea\u30fc\u30af\u304c\u5c11\u306a\u304f\u3001\u5fc5\u8981\u306a\u30ea\u30ac\u30f3\u30c9\u6fc3\u5ea6\u3082\u4f4e\u304f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u306b\u5fc5\u8981\u306a\u6642\u9593\u3082\u77ed\u7e2e\u3055\u308c\u3066\u3044\u308b\u306e\u304c\u7279\u5fb4\u3067\u3059\u3002
\n\u7406\u7814BRC\u5b9f\u9a13\u52d5\u7269\u958b\u767a\u5ba4\u306b\u306f\u3001CAG\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u5236\u5fa1\u4e0b\u3067\u3001TIR1(F74G)\u3068\u30c6\u30b0\u30ed\u30f3\u30bf\u30b0AID\u53ca\u3073\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceaEGFP\u3092\u767a\u73fe\u3059\u308b\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30de\u30a6\u30b9(RBRC11438, RBRC11439)\u304c\u5bc4\u8a17\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u672c\u7cfb\u7d71\u3067\u306f\u3001\u30de\u30a6\u30b9\u8179\u8154\u5185\u306b\u30ea\u30ac\u30f3\u30c95-Ph-IAA\u3092\u6295\u4e0e\u3059\u308b\u3053\u3068\u3067\u3001\u30de\u30a6\u30b9\u751f\u4f53\u5185\u3067\u3001AID2\u6cd5\u3092\u5b9f\u73fe\u3057\u3001\u305d\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u306e\u7a0b\u5ea6\u3092\u86cd\u5149\u30e2\u30cb\u30bf\u30fc\u53ef\u80fd\u3067\u3059\u3002AID2\u6cd5\u306f\u30bf\u30f3\u30d1\u30af\u30ec\u30d9\u30eb\u3067\u306e\u767a\u73fe\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u3092\u53ef\u80fd\u306b\u3059\u308b\u65b0\u898f\u6280\u8853\u3067\u3042\u308a\u3001\u4eca\u5f8c\u3001\u751f\u547d\u79d1\u5b66\u7814\u7a76\u306b\u304a\u3044\u3066\u3060\u3051\u3067\u306a\u304f\u3001\u533b\u5b66\u3001\u5275\u85ac\u5206\u91ce\u306a\u3069\u69d8\u3005\u306a\u5206\u91ce\u3067\u306e\u5fdc\u7528\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Depositor<\/td>\n:<\/td>\n\u76f8\u8cc0 \u88d5\u7f8e\u5b50 \u5148\u751f
\u56fd\u7acb\u907a\u4f1d\u5b66\u7814\u7a76\u6240\n<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nB6;B6D2-Tg(CAG-OsTIR1-P2A-mAID-EGFP-nanoluc)#16Ysa\n<\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC11438<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nB6;B6D2-Tg(CAG-OsTIR1-P2A-mAID-EGFP-nanoluc)#19Ysa\n<\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC11439<\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\n[1]<\/td>\nYesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT.
\nThe auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice.
\nNat Commun.<\/em> 2020 Nov 11;11(1):5701.<\/a><\/td>\n<\/tr>\n
[2]<\/td>\nNishimura K, Fukagawa T, Takisawa H, Kakimoto T, Kanemaki M.
\nAn auxin-based degron system for the rapid depletion of proteins in nonplant cells.
\nNat Methods.<\/em> 2009 Dec;6(12):917-22.<\/a><\/td>\n<\/tr>\n
[3]<\/td>\nNatsume T, Kiyomitsu T, Saga Y, Kanemaki MT.
\nRapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.
\nCell Rep.<\/em> 2016 Apr 5;15(1):210-218.<\/a><\/td>\n<\/tr>\n
Related program<\/td>\n:<\/td>\nNBRP\u57fa\u76e4\u6280\u8853\u6574\u5099\u30d7\u30ed\u30b0\u30e9\u30e0<\/a><\/td>\n<\/tr>\n
Related DNA resources in RIKEN BRC<\/td>\n:<\/td>\n\u30aa\u30fc\u30ad\u30b7\u30f3\u30c7\u30b0\u30ed\u30f3\u6cd5 (AID system) \u306b\u3088\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u5236\u5fa1<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
February 2022
\nSaori Mizuno, Ph.D.
\nContact: Experimental Animal Division, RIKEN BioResource Research Center (animal.brc@riken.jp)<\/a>
\nAll materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

February 2022 Mouse of the Month \u65b0\u898f\u30bf\u30f3\u30d1\u30af\u8cea\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u6280\u8853\u30aa\u30fc\u30ad\u30b7\u30f3\u30c7\u30b0\u30ed\u30f3(AID) 2\u6cd5 B6;B6D2-Tg(CAG-OsTIR1-P2A-mAID-EGFP-nanoluc)#16Ysa (RBRC11438) B6;B6D2-Tg(CAG-OsTIR1-P2A-mAID-EGFP-nanoluc)#19Ysa (RBRC11439) Courtesy […]<\/p>\n","protected":false},"author":19,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-18839","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/18839","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=18839"}],"version-history":[{"count":3,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/18839\/revisions"}],"predecessor-version":[{"id":19369,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/18839\/revisions\/19369"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=18839"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}