{"id":19038,"date":"2022-06-15T10:13:11","date_gmt":"2022-06-15T01:13:11","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=19038"},"modified":"2022-06-15T10:13:11","modified_gmt":"2022-06-15T01:13:11","slug":"jun_2022","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/current_tech\/jun_2022","title":{"rendered":"BRC Current Technology June 2022"},"content":{"rendered":"\n\n\n\n\n
\"RIKEN<\/td>\nBRC Current Technology<\/span>
\nJune 2022
\n<\/span><\/a><\/td>\n<\/tr>\n
\n

18.\u3000\u30de\u30a6\u30b9\u306e\u7523\u5b50\u6570\u30921.4\u500d\u306b\u5897\u52a0\u3055\u305b\u308b\u3053\u3068\u306b\u6210\u529f<\/h1>\n

\u598a\u5a20\u30fb\u51fa\u7523\u306b\u5f71\u97ff\u3057\u306a\u3044\u6297\u30a4\u30f3\u30d2\u30d3\u30f3\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3092\u958b\u767a<\/h2>\n
\n\"(a)(b)(c)(d)\"<\/a><\/p>\n<\/div>\n

(a) \u6297\u30a4\u30f3\u30d2\u30d3\u30f3\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53 (AIMA) \u3092\u7528\u3044\u305f\u904e\u6392\u5375\u51e6\u7f6e\u3068\u4ea4\u914d\u306e\u30b9\u30b1\u30b8\u30e5\u30fc\u30eb\u3002(b) AIMA \u6295\u4e0e\u306b\u3088\u308a\u30de\u30a6\u30b9\u3067\u96cc1\u5339\u304b\u3089\u306e\u7523\u5b50\u6570\u304c1.4\u500d\u306b\u6709\u610f\u306b\u5897\u52a0\u3002(c) AIMA\u6295\u4e0e\u5f8c\u306b in vivo \u30b2\u30ce\u30e0\u7de8\u96c6 (i<\/i>-GONAD) \u3092\u5b9f\u65bd\u3059\u308b\u3053\u3068\u3067\u3001\u975e\u6295\u4e0e\u7fa4\u3088\u308a\u7523\u5b50\u6570\u304c\u6539\u5584\u3002(d,e) i<\/i>-GONAD \u306b\u3088\u308a\u30c1\u30ed\u30b7\u30ca\u30fc\u30bc\u907a\u4f1d\u5b50\u3092\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u3055\u308c\u305f\u30a2\u30eb\u30d3\u30ce\uff08\u767d\u8272\uff09\u306e\u65b0\u751f\u4ed4\u304a\u3088\u3073\u5b50\u3002*\u6709\u610f\u5dee\u3042\u308a(P<\/I> < 0.05)<\/p>\n<\/td>\n<\/tr>\n

\u54fa\u4e73\u52d5\u7269\u3067\u306f\u5375\u80de\u523a\u6fc0\u30db\u30eb\u30e2\u30f3(FSH)\u306a\u3069\u306b\u3088\u308a\u3001\u52d5\u7269\u7a2e\u6bce\u306b\u96cc1\u5339\u304b\u3089\u7523\u307e\u308c\u308b\u5b50\u4f9b\u306e\u6570\u304c\u6c7a\u3081\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u79c1\u9054\u306fFSH\u306e\u5206\u6ccc\u91cf\u3092\u6291\u5236\u3059\u308b\u50cd\u304d\u3092\u6301\u3064\u30a4\u30f3\u30d2\u30d3\u30f3\u3092\u7121\u52b9\u5316\u3059\u308b\u305f\u3081\u306b\u3001\u6297\u30a4\u30f3\u30d2\u30d3\u30f3\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53(AIMA)\u3092\u4f5c\u88fd\u30fb\u9078\u629c\u3057\u3001\u9069\u671f\u306b\u6295\u4e0e\u3059\u308b\u3053\u3068\u3067\u7523\u5b50\u6570\u3092\u5897\u52a0\u3055\u305b\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u307e\u3057\u305f [1]\u3002C57BL\/6J\u7cfb\u7d71\u3068ICR\u7cfb\u7d71\u3067\u7d041.4\u500d\u306b\u5897\u52a0\u3057\u3001\u6b63\u5e38\u306b\u767a\u80b2\u3059\u308b\u7523\u5b50\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\u3002\u672c\u6cd5\u3092\u4f53\u5185\u96fb\u6c17\u7a7f\u5b54\u6cd5(i<\/i>-GONAD)\u306b\u3088\u308b\u30b2\u30ce\u30e0\u7de8\u96c6 [2] \u306b\u5fdc\u7528\u3057\u305f\u3068\u3053\u308d\u3001i<\/i>-GONAD\u51e6\u7f6e\u5f8c\u306e\u7523\u5b50\u6570\u304c1.5\u500d\u306b\u5897\u3048\u3001\u975e\u6295\u4e0e\u7fa4\u3068\u540c\u7b49\u306e\u5909\u7570\u7387\u304c\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\uff08\u6771\u6d77\u5927\u5b66\u5927\u585a\u6b63\u4eba\u6559\u6388\u3089\u3068\u306e\u5171\u540c\u7814\u7a76\uff09[1]\u3002
\n\u3000\u3053\u306eAIMA\u6295\u4e0e\u306b\u3088\u308b\u7523\u5b50\u4f5c\u51fa\u65b9\u6cd5\u306f\u3001\u7e41\u6b96\u52b9\u7387\u6539\u5584\u304c\u5fc5\u8981\u306a\u30de\u30a6\u30b9\u7cfb\u7d71\u3084\u9ad8\u9f62\u30de\u30a6\u30b9\u3001i<\/i>-GONAD\u7b49\u306e\u51e6\u7f6e\u306e\u305f\u3081\u306e\u7e41\u6b96\u6210\u7e3e\u306e\u6539\u5584\u306a\u3069\u306b\u52b9\u679c\u7684\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n
References:<\/td>\n[1]<\/td>\nA Hasegawa et al.<\/i>, \u201cUse of anti-inhibin monoclonal antibody for increasing the litter size of mouse strains and its application to in vivo<\/i>-genome editing technology,\u201d
\nBiol. Reprod.<\/i> 2022 Apr 2:ioac068. doi: 10.1093\/biolre\/ioac068.<\/a><\/td>\n<\/tr>\n
[2]<\/td>\nM. Ohtsuka et al.<\/i>, \u201ci-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases,\u201d
\nGenome Biol.<\/i> 2018 Feb 26;19(1):25. doi: 10.1186\/s13059-018-1400-x.
\n<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

BRC Current Technology June 2022 18.\u3000\u30de\u30a6\u30b9\u306e\u7523\u5b50\u6570\u30921.4\u500d\u306b\u5897\u52a0\u3055\u305b\u308b\u3053\u3068\u306b\u6210\u529f \u598a\u5a20\u30fb\u51fa\u7523\u306b\u5f71\u97ff\u3057\u306a\u3044\u6297\u30a4\u30f3\u30d2\u30d3\u30f3\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3092\u958b\u767a (a) \u6297\u30a4\u30f3\u30d2\u30d3\u30f3\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53 (AIMA) \u3092\u7528\u3044\u305f\u904e\u6392\u5375\u51e6\u7f6e\u3068\u4ea4\u914d\u306e\u30b9\u30b1\u30b8\u30e5\u30fc\u30eb\u3002(b) AIMA \u6295\u4e0e\u306b\u3088\u308a\u30de\u30a6\u30b9\u3067\u96cc1\u5339\u304b\u3089\u306e\u7523\u5b50\u6570\u304c1.4\u500d\u306b\u6709\u610f\u306b\u5897\u52a0\u3002(c) AIMA\u6295\u4e0e\u5f8c\u306b in vivo \u30b2\u30ce […]<\/p>\n","protected":false},"author":19,"featured_media":0,"parent":3268,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-19038","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/19038","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=19038"}],"version-history":[{"count":1,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/19038\/revisions"}],"predecessor-version":[{"id":19039,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/19038\/revisions\/19039"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3268"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=19038"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}