{"id":19222,"date":"2022-08-18T13:39:12","date_gmt":"2022-08-18T04:39:12","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=19222"},"modified":"2023-05-22T17:01:59","modified_gmt":"2023-05-22T08:01:59","slug":"mouse_es","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_es","title":{"rendered":"The Gene-trap Mouse ES cell clones"},"content":{"rendered":"<p>\n\u3000\u5948\u826f\u5148\u7aef\u79d1\u5b66\u6280\u8853\u5927\u5b66\u9662\u5927\u5b66\u30fb\u77f3\u7530\u9756\u96c5\u535a\u58eb\u306e\u958b\u767a\u3057\u305f\u907a\u4f1d\u5b50\u30c8\u30e9\u30c3\u30d7\u6cd5 UPATrap \u306b\u3088\u308b\u5909\u7570\u578b\u30de\u30a6\u30b9ES\u7d30\u80de\u682a\u304c\u5bc4\u8a17\u3055\u308c\u307e\u3057\u305f\u3002 In vitro\u5206\u5316\u8a98\u5c0e\u5b9f\u9a13\u3084\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u306e\u4f5c\u88fd\u306a\u3069\u306b\u6709\u7528\u3067\u3059\u3002<br \/>\n\u3000\u3054\u4f9d\u983c\u3092\u3044\u305f\u3060\u3044\u3066\u304b\u3089\u57f9\u990a\u3001\u54c1\u8cea\u691c\u67fb\u7b49\u3092\u5b9f\u65bd\u3057\u3001\u63d0\u4f9b\u7528\u51cd\u7d50\u4fdd\u5b58\u7d30\u80de\u3092\u4f5c\u88fd\u3044\u305f\u3057\u307e\u3059\u3002\u5f93\u3044\u307e\u3057\u3066\u3001\u3054\u4f9d\u983c\u3092\u53d7\u3051\u3066\u304b\u3089\u63d0\u4f9b\u307e\u3067\u306b3\u30f6\u6708\u7a0b\u5ea6\u306e\u6642\u9593\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3053\u3068\u3092\u4e88\u3081\u3054\u4e86\u627f\u304f\u3060\u3055\u3044\u3002\n<\/p>\n<ul>\n<li>ES cell: (C57BL\/6J x 129\/Sv<sup>Jae<\/sup>)F1 embryonic stem cell\n<li>\u4f7f\u7528\u6761\u4ef6\n<ol>\n<li>\u672c\u4ef6\u30ea\u30bd\u30fc\u30b9\u306e\u5229\u7528\u306f\u5b66\u8853\u7814\u7a76\u6a5f\u95a2\u306e\u975e\u55b6\u5229\u76ee\u7684\u306e\u7814\u7a76\u306b\u9650\u308b\u3002\u4f01\u696d\u3068\u5171\u540c\u7814\u7a76\u3092\u5b9f\u65bd\u3059\u308b\u5834\u5408\u306f\u6b21\u9805\u3068\u540c\u3058\u6271\u3044\u3068\u3057\u3001\u4e8b\u524d\u306b\u5bc4\u8a17\u6a5f\u95a2\u306e\u627f\u8afe\u3092\u5f97\u3066\u3001\u627f\u8afe\u66f8\u306e\u5199\u3057\u3092\u7406\u7814BRC\u306b\u63d0\u51fa\u3059\u308b\u3002\n<li>\u672c\u4ef6\u30ea\u30bd\u30fc\u30b9\u3092\u975e\u5b66\u8853\u7814\u7a76\u6a5f\u95a2\u304c\u5229\u7528\u3059\u308b\u3053\u3068\u3092\u5e0c\u671b\u3059\u308b\u5834\u5408\u306b\u306f\u3001\u4e8b\u524d\u306b\u5bc4\u8a17\u6a5f\u95a2\u306e\u627f\u8afe\u3092\u5f97\u3066\u3001\u627f\u8afe\u66f8\u306e\u5199\u3057\u3092\u7406\u7814BRC\u306b\u63d0\u51fa\u3059\u308b\u3002\n<li>\u5229\u7528\u8005\u306f\u3001\u7814\u7a76\u6210\u679c\u306e\u516c\u8868\u306b\u3042\u305f\u3063\u3066\u3001\u672c\u4ef6\u30ea\u30bd\u30fc\u30b9\u958b\u767a\u8005\uff08\u77f3\u7530\u9756\u96c5\u535a\u58eb\uff09\u3078\u306e\u8b1d\u8f9e\u3092\u8868\u660e\u3059\u308b\u3002\n<li>\u5229\u7528\u8005\u306f\u3001\u7814\u7a76\u6210\u679c\u306e\u516c\u8868\u306b\u3042\u305f\u3063\u3066\u3001\u5bc4\u8a17\u6a5f\u95a2\u306e\u6307\u5b9a\u3059\u308b\u6b21\u306e\u6587\u732e\uff08Nucleic Acids Res. 2005;33:e20\uff09\u3092\u5f15\u7528\u3059\u308b\u3002\n<li>\u5229\u7528\u8005\u304c\u672c\u4ef6\u30ea\u30bd\u30fc\u30b9\u3092\u4f7f\u7528\u3057\u3066\u5f97\u3089\u308c\u305f\u7814\u7a76\u6210\u679c\u306b\u57fa\u3065\u304d\u7279\u8a31\u7b49\u306e\u7533\u8acb\u53ca\u3073\u5546\u696d\u6d3b\u52d5\u3092\u884c\u3046\u5834\u5408\u306b\u306f\u3001\u5bc4\u8a17\u6a5f\u95a2\u3068\u5225\u9014\u5354\u8b70\u3092\u884c\u3046\u3002\n<li>\u5229\u7528\u8005\u306f\u3001\u672c\u4ef6\u30ea\u30bd\u30fc\u30b9\u3092\u7528\u3044\u3066\u30de\u30a6\u30b9\u500b\u4f53\u3092\u4f5c\u51fa\u3057\u305f\u5834\u5408\u306b\u306f\u3001\u5f53\u8a72\u30de\u30a6\u30b9\u3092\u7406\u7814BRC\u306b\u5bc4\u8a17\u3059\u308b\u3002\n    <\/ol>\n<li>\u57f9\u5730\uff1aDMEM(high glucose, Sodium pyruvate(-))<br \/>\u3000+15%FBS+0.1mM NEAA<br \/>\u3000+0.1mM 2-Mercaptoethanol<br \/>\u3000+1000U\/ml LIF<br \/>Feeder:SNL\u7d30\u80de\uff08SNL 76\/7\u7b49\uff09\n<li>\u57f9\u990a\u306b\u95a2\u3059\u308b\u554f\u3044\u5408\u308f\u305b\u3001\u5229\u7528\u7533\u8fbc\u65b9\u6cd5&nbsp;<a href=\"mailto:cellqa.brc@riken.jp\">cellqa.brc@riken.jp<\/a>&nbsp;\u306b\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044\u3002\n<\/ul>\n<hr \/>\n<ul>\n<li><a href=\"\/ja\/wp-content\/uploads\/data\/ishida_es.html\">\u7d30\u80de\u682a\u306e\u30ea\u30b9\u30c8<\/a>\n<li><a href=\"https:\/\/bsw3.naist.jp\/kawaichi\/naistrap.html\">The NAISTrap Project<\/a>\n<li><a href=\"https:\/\/bsw3.naist.jp\/kawaichi\/kawaiti.html\">\u5948\u826f\u5148\u7aef\u79d1\u5b66\u6280\u8853\u5927\u5b66\u9662\u5927\u5b66\u3000\u30d0\u30a4\u30aa\u30b5\u30a4\u30a8\u30f3\u30b9\u7814\u7a76\u79d1\u3000\u52d5\u7269\u907a\u4f1d\u5b50\u6a5f\u80fd\u5b66\u8b1b\u5ea7<\/a>\n<li><a href=\"https:\/\/igtc.org\">International Gene Trap Consortium (IGTC)<\/a>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/nuccore?term=NAIST\">NCBI GSS Search<\/a>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u3000\u5948\u826f\u5148\u7aef\u79d1\u5b66\u6280\u8853\u5927\u5b66\u9662\u5927\u5b66\u30fb\u77f3\u7530\u9756\u96c5\u535a\u58eb\u306e\u958b\u767a\u3057\u305f\u907a\u4f1d\u5b50\u30c8\u30e9\u30c3\u30d7\u6cd5 UPATrap \u306b\u3088\u308b\u5909\u7570\u578b\u30de\u30a6\u30b9ES\u7d30\u80de\u682a\u304c\u5bc4\u8a17\u3055\u308c\u307e\u3057\u305f\u3002 In vitro\u5206\u5316\u8a98\u5c0e\u5b9f\u9a13\u3084\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u30de\u30a6\u30b9\u306e\u4f5c\u88fd\u306a\u3069\u306b\u6709\u7528\u3067\u3059\u3002 \u3000\u3054\u4f9d\u983c\u3092\u3044\u305f\u3060\u3044\u3066\u304b\u3089\u57f9\u990a\u3001\u54c1\u8cea\u691c\u67fb\u7b49\u3092\u5b9f\u65bd\u3057\u3001\u63d0\u4f9b\u7528\u51cd\u7d50\u4fdd\u5b58\u7d30\u80de\u3092\u4f5c\u88fd\u3044\u305f\u3057\u307e\u3059\u3002\u5f93\u3044\u307e\u3057\u3066\u3001\u3054\u4f9d\u983c\u3092\u53d7\u3051\u3066\u304b\u3089\u63d0\u4f9b\u307e\u3067\u306b3\u30f6\u6708\u7a0b\u5ea6\u306e\u6642\u9593\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3053\u3068\u3092\u4e88\u3081\u3054\u4e86\u627f\u304f\u3060\u3055\u3044\u3002 ES c [&hellip;]<\/p>\n","protected":false},"author":12,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-19222","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/19222","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=19222"}],"version-history":[{"count":3,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/19222\/revisions"}],"predecessor-version":[{"id":19663,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/19222\/revisions\/19663"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=19222"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}