{"id":20134,"date":"2024-04-01T11:19:58","date_gmt":"2024-04-01T02:19:58","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=20134"},"modified":"2024-04-10T14:08:18","modified_gmt":"2024-04-10T05:08:18","slug":"apr_2024_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/apr_2024_mm","title":{"rendered":"CD103\u967d\u6027\u6a39\u72b6\u7d30\u80de\u3068\u6d88\u5316\u7ba1\u7c98\u819c\u514d\u75ab\u5bdb\u5bb9\u306e\u95a2\u4fc2\u6027"},"content":{"rendered":"\n\n\n\n\n\n\n
\"RIKEN<\/td>\nApril 2024<\/span>
\nMouse of the Month<\/span><\/a><\/td>\n<\/tr>\n<\/thead>\n
\n

CD103\u967d\u6027\u6a39\u72b6\u7d30\u80de\u3068\u6d88\u5316\u7ba1\u7c98\u819c\u514d\u75ab\u5bdb\u5bb9\u306e\u95a2\u4fc2\u6027<\/p>\n

C57BL\/6-Tg(Itgae-HBEGF\/EGFP)1Ksat (RBRC11949)<\/a>
C57BL\/6-Tnfsf15<em1Ksat> (RBRC11950)<\/a>
\n<\/h5>\n

\"A,B,C\"<\/a>\n <\/p>\n

CD11c-Cre:CD103-LSL-DTR\u30de\u30a6\u30b9\u3068Tnfsf15<\/i>\u907a\u4f1d\u5b50\u6b20\u640d\u30de\u30a6\u30b9\u306e\u7279\u5fb4\u3002
\n(A, B)DT\u3092\u6295\u4e0e\u3057\u305fCD 11c-Cre:CD103-LSL-DTR\u30de\u30a6\u30b9\u306b\u304a\u3051\u308b\u8178\u9593\u819c\u30ea\u30f3\u30d1\u7bc0\u306e\u514d\u75ab\u5b66\u7684\u89e3\u6790\u3002
\n(A)\u8178\u9593\u819c\u30ea\u30f3\u30d1\u7bc0\u3092\u6297CD11c\u6297\u4f53(\u7dd1)\u3001\u6297CD103\u6297\u4f53(\u8d64)\u3001\u6297CD19\u6297\u4f53(\u9752)\u3092\u7528\u3044\u3066\u514d\u75ab\u86cd\u5149\u67d3\u8272\u89e3\u6790\u3057\u305f\u3002DT\u6295\u4e0e\u306b\u3088\u308aCD11c+CD103+cDCs\uff08\u9ec4[\u7dd1+\u8d64]\uff09\u306e\u7279\u7570\u7684\u6d88\u5931\u304c\u8a8d\u3081\u3089\u308c\u308b\u3002
\n(B)\u8178\u9593\u819c\u30ea\u30f3\u30d1\u7bc0\u767d\u8840\u7403\u3092\u6297CD11c\u6297\u4f53\u3068\u6297CD103\u6297\u4f53\u3092\u7528\u3044\u3066\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u89e3\u6790\u3057\u305f\u3002DT\u6295\u4e0e\u306b\u3088\u308aCD11c+<\/sup>CD103+<\/sup>cDCs\u306e\u7279\u7570\u7684\u6d88\u5931\u304c\u8a8d\u3081\u3089\u308c\u308b\u3002\u30c9\u30c3\u30c8\u30d7\u30ed\u30c3\u30c8\u4e2d\u306e\u6570\u5024\u306f\u8178\u9593\u819c\u30ea\u30f3\u30d1\u7bc0\u767d\u8840\u7403\u4e2d\u306eCD11c+<\/sup>CD103+<\/sup>cDCs\u306e\u5272\u5408(%)\u3002
\n(C) Tnfsf15<\/i>\u907a\u4f1d\u5b50\u6b20\u640d\u30de\u30a6\u30b9\u306b\u304a\u3051\u308b\u8178\u9593\u819c\u30ea\u30f3\u30d1\u7bc0\u306eCD11c+<\/sup>cDCs\u3092\u6297TLA1\u6297\u4f53\u3092\u7528\u3044\u3066\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u89e3\u6790\u3057\u305f\u3002\u91ce\u751f\u578b\uff08wild-type; WT\uff09\u30de\u30a6\u30b9\u306e\u8178\u9593\u819c\u30ea\u30f3\u30d1\u7bc0\u306eCD11c+<\/sup>cDCs\u3067\u306fTL1A\u306e\u767a\u73fe\u304c\u8a8d\u3081\u3089\u308c\u308b\u304c\u3001Tnfsf15<\/i>\u907a\u4f1d\u5b50\u6b20\u640d(TL1A KO)\u30de\u30a6\u30b9\u306e\u8178\u9593\u819c\u30ea\u30f3\u30d1\u7bc0\u306eCD11c+<\/sup>cDCs\u3067\u306fTL1A\u306e\u767a\u73fe\u304c\u8a8d\u3081\u3089\u308c\u306a\u3044\u3002\n<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n

2023\u5e745\u6708\u3001\u5bc4\u8a17\u8005\u306e\u4f50\u85e4\u514b\u660e\u5148\u751f\u3089\u306f\u3001\u6297\u751f\u5264\u670d\u7528\u306b\u3088\u3063\u3066\u5f15\u304d\u8d77\u3053\u3055\u308c\u308b\u6d88\u5316\u7ba1\u7d30\u83cc\u53e2\u7570\u5e38\u304c\u3001\u30a2\u30ec\u30eb\u30ae\u30fc\u767a\u75c7\u30ea\u30b9\u30af\u306e\u5897\u52a0\u306b\u7e4b\u304c\u308b\u6d88\u5316\u7ba1\u7c98\u819c\u514d\u75ab\u5bdb\u5bb9\u306e\u7834\u7dbb\u3092\u5c0e\u304f\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u767a\u898b\u3057\u3001\u767a\u8868\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u7814\u7a76\u6210\u679c\u306f\u3001\u30a2\u30ec\u30eb\u30ae\u30fc\u306b\u5bfe\u3059\u308b\u65b0\u898f\u6cbb\u7642\u6cd5\u958b\u767a\u306e\u7cf8\u53e3\u3068\u3057\u3066\u6ce8\u76ee\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4eca\u56de\u306f\u3001\u3053\u306e\u7814\u7a76\u3067\u7528\u3044\u3089\u308c\u305f\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30a6\u30b92\u7cfb\u7d71\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002
\nB6.CD103-LSL-DTR\u30de\u30a6\u30b9(RBRC11949)\u306f\u3001Cd103<\/i>\u907a\u4f1d\u5b50\u30a8\u30af\u30bd\u30f331\u306e\u76f4\u4e0b\u306b\u3001loxP\u914d\u5217\u306b\u631f\u307e\u308c\u305fSTOP\u914d\u5217(LSL\u30ab\u30bb\u30c3\u30c8)\u3068\u3001\u30d2\u30c8\u30b8\u30d5\u30c6\u30ea\u30a2\u6bd2\u7d20\u53d7\u5bb9\u4f53(diphtherial toxin [DT] receptor; DTR)-EGFP\u30ab\u30bb\u30c3\u30c8\u3092\u6301\u3064BAC Tg\u30de\u30a6\u30b9\u3067\u3059\u3002CD103\u967d\u6027\u7d30\u80de\u306b\u304a\u3044\u3066\u3001Cre\u7d44\u307f\u63db\u3048\u9175\u7d20\u5b58\u5728\u4e0b\u3067DTR\u3092\u767a\u73fe\u3057\u3001DT\u6295\u4e0e\u6642\u306bCD103\u967d\u6027\u7d30\u80de\u306e\u6b20\u5931\u304c\u8a98\u5c0e\u3055\u308c\u307e\u3059\u3002CD103\u306f\u30a4\u30f3\u30c6\u30b0\u30ea\u30f3\u30b9\u30fc\u30d1\u30fc\u30d5\u30a1\u30df\u30ea\u30fc\u306b\u5c5e\u3059\u308b\u7d30\u80de\u63a5\u7740\u5206\u5b50\u3067\u3001E-\u30ab\u30c9\u30d8\u30ea\u30f3\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u3057\u3001\u514d\u75ab\u7d30\u80de\u306e\u76ae\u819a\u7d44\u7e54\u3084\u7c98\u819c\u7d44\u7e54\u3078\u306e\u5c40\u5728\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u4f50\u85e4\u5148\u751f\u3089\u306f\u3001B6.CD103-LSL-DTR\u30de\u30a6\u30b9\u3068\u3001\u901a\u5e38\u578b\u6a39\u72b6\u7d30\u80de(conventional dendritic cells; cDCs)\u3067\u767a\u73fe\u3059\u308bCd11c<\/i>\u907a\u4f1d\u5b50\u306eCre\u30c9\u30e9\u30a4\u30d0\u30fc\u30de\u30a6\u30b9\u3092\u4ea4\u914d\u3057\u3001DT\u3092\u6295\u4e0e\u3057\u3066\u3001CD103\u967d\u6027cDCs\u6b20\u640d\u30de\u30a6\u30b9\u3092\u4f5c\u88fd\u3057\u3001CD103\u967d\u6027cDCs\u306e\u6a5f\u80fd\u89e3\u6790\u3092\u884c\u3044\u307e\u3057\u305f\u3002
\nTnfsf15<\/i>\u907a\u4f1d\u5b50\u6b20\u640d\u30de\u30a6\u30b9(RBRC11950)\u306f\u3001CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u3092\u7528\u3044\u3066\u3001Tnfsf15<\/i>\u907a\u4f1d\u5b50\u306e\u30a8\u30af\u30bd\u30f32, 3\u3092\u542b\u30803,014bp\u304c\u6b20\u5931\u3057\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u30de\u30a6\u30b9\u3067\u3059\u3002\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3TNF(tumor necrosis factor)\u30d5\u30a1\u30df\u30ea\u30fc\u306b\u5c5e\u3059\u308bTnfsf15<\/i>\u907a\u4f1d\u5b50\u306f\u3001TL1A(TNF-like ligand 1a)\u3092\u30b3\u30fc\u30c9\u3057\u307e\u3059\u3002TL1A\u306e\u53d7\u5bb9\u4f53\u306fTNF\u53d7\u5bb9\u4f53\u30d5\u30a1\u30df\u30ea\u30fc\u306e\u3046\u3061\u306eDR3(TNFRSF25)\u3067\u3059\u3002TL1A\u306fcDCs\u3084\u30de\u30af\u30ed\u30d5\u30a1\u30fc\u30b8\u304b\u3089\u7523\u751f\u3055\u308c\u3001TL1A \u306e\u53d7\u5bb9\u4f53\u3067\u3042\u308bDR3(TNFRSF25)\u304c\u9ad8\u767a\u73fe\u3059\u308bT\u7d30\u80de\u3084\u81ea\u7136\u30ea\u30f3\u30d1\u7403\u7b49\u306b\u4f5c\u7528\u3057\u3001\u708e\u75c7\u6027\u514d\u75ab\u75be\u60a3\u306b\u95a2\u4e0e\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u4f50\u85e4\u5148\u751f\u3089\u306f\u3001Tnfsf15<\/i>\u907a\u4f1d\u5b50\u6b20\u640d\u30de\u30a6\u30b9\u3092\u7528\u3044\u3066\u3001\u8178\u9593\u819c\u30ea\u30f3\u30d1\u7bc0\u3067\u306eTL1A\u7523\u751f\u4f4e\u4e0b\u3068\u3001\u514d\u75ab\u6291\u5236\u6a5f\u80fd\u55aa\u5931\u3068\u306e\u95a2\u4fc2\u6027\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002
\nB6.CD103-LSL-DTR\u30de\u30a6\u30b9\u3082Tnfsf15<\/i>\u907a\u4f1d\u5b50\u6b20\u640d\u30de\u30a6\u30b9\u3082\u3001\u514d\u75ab\u5b66\u5206\u91ce\u3068\u3001\u305d\u308c\u306b\u95a2\u308f\u308b\u75be\u60a3\u7814\u7a76\u306b\u3001\u65b0\u305f\u306a\u77e5\u898b\u3092\u3082\u305f\u3089\u3059\u3053\u3068\u304c\u671f\u5f85\u3055\u308c\u308b\u3001\u6709\u7528\u306a\u7cfb\u7d71\u3067\u3059\u3002\n <\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n
Keywords<\/td>\n:<\/td>\n\u6d88\u5316\u7ba1\u7d30\u83cc\u53e2\u7570\u5e38\u3001\u6d88\u5316\u7ba1\u7c98\u819c\u514d\u75ab\u5bdb\u5bb9\u3001\u30a2\u30ec\u30eb\u30ae\u30fc\u3001CD103\u3001TL1A<\/td>\n<\/tr>\n
Depositor<\/td>\n:<\/td>\n\u4f50\u85e4\u514b\u660e\u5148\u751f(\u5bae\u5d0e\u5927\u5b66)<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nC57BL\/6-Tg(Itgae-HBEGF\/EGFP)1Ksat<\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC11949<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nC57BL\/6-Tnfsf15<em1Ksat><\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC11950<\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\n[1]<\/td>\nFukaya T, Uto T, Mitoma S, Takagi H, Nishikawa Y, Tominaga M, Choijookhuu N, Hishikawa Y, Sato K.
\nGut dysbiosis promotes the breakdown of oral tolerance mediated through dysfunction of mucosal dendritic cells.
\nCell Rep.<\/em> 2023 May 30;42(5):112431.\n <\/td>\n<\/tr>\n
[2]<\/td>\n\u5e7c\u82e5\u671f\u306e\u6297\u751f\u5264\u670d\u7528\u304b\u3099\u30a2\u30ec\u30eb\u30ad\u3099\u30fc\u767a\u75c7\u30ea\u30b9\u30af\u3092\u9ad8\u3081\u308b\u4ed5\u7d44\u307f\u3092\u767a\u898b-\u5e7c\u82e5\u671f\u306e\u6297\u751f\u5264\u670d\u7528\u306b\u3088\u308b\u6d88\u5316\u7ba1\u7d30\u83cc\u53e2\u7570\u5e38\u304b\u3099\u6d88\u5316\u7ba1\u7c98\u819c\u514d\u75ab\u5bdb\u5bb9\u306e\u7834\u7dbb\u3092\u5c0e\u304f\u2015
\n 2023\u5e744\u670828\u65e5\u5bae\u5d0e\u5927\u5b66\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
April 2024
\nSaori Mizuno, Ph.D.
\nContact: Experimental Animal Division, RIKEN BioResource Research Center (animal.brc@riken.jp)<\/a>
\nAll materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":19,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"https:\/\/mus.brc.riken.jp\/ja\/wp-content\/uploads\/2024\/04\/MMfig_202404.jpeg","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"https:\/\/mus.brc.riken.jp\/ja\/wp-content\/uploads\/2024\/04\/MMfig_202404.jpeg","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-20134","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/20134","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=20134"}],"version-history":[{"count":1,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/20134\/revisions"}],"predecessor-version":[{"id":20135,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/20134\/revisions\/20135"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=20134"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}