{"id":20434,"date":"2024-12-04T09:45:04","date_gmt":"2024-12-04T00:45:04","guid":{"rendered":"http:\/\/mus.brc.riken.jp\/ja\/?page_id=20434"},"modified":"2024-12-05T09:49:39","modified_gmt":"2024-12-05T00:49:39","slug":"dec_2024_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/dec_2024_mm","title":{"rendered":"\u751f\u4f53\u5185\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u3092\u89e3\u6790\u53ef\u80fd\u306a in vivo<\/i> BioID\u30de\u30a6\u30b9"},"content":{"rendered":"\n\n\n\n\n\n\n
\"RIKEN<\/td>\nDecember 2024<\/span>
\nMouse of the Month<\/span><\/a><\/td>\n<\/tr>\n<\/thead>\n
\n

\u751f\u4f53\u5185\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u3092\u89e3\u6790\u53ef\u80fd\u306a
in vivo<\/i> BioID\u30de\u30a6\u30b9<\/p>\n

C57BL\/6J-Gt(ROSA)26Sor<em1(floxed-EGFP-Arntl-BirA<*>)Murk> (RBRC12179)<\/a>
C57BL\/6J-Gt(ROSA)26Sor<em1.1(floxed-EGFP-Arntl-BirA<*>)Murk> (RBRC12180)<\/a>
\n<\/h5>\n

\"A.B.\"<\/a>\n <\/p>\n

A. EGFPflox<\/sup>-BMAL1-BioID\u30de\u30a6\u30b9\u306e\u6982\u8981\u3002Cre \u30de\u30a6\u30b9\u3068\u306e\u4ea4\u914d\u306a\u3069\u306b\u3088\u308a\u7d30\u80de\u30fb\u7d44\u7e54\u7279\u7570\u7684\u306b BMAL1-BirA*<\/sup>-HA \u878d\u5408\u30bf\u30f3\u30d1\u30af\u3092\u767a\u73fe\u3055\u305b\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3042\u308b\u3002
\nB. BMAL1-BioID\u30de\u30a6\u30b9\u306e\u6982\u8981\u3002\u5168\u8eab\u6027\u306b BMAL1-BirA*<\/sup>-HA \u878d\u5408\u30bf\u30f3\u30d1\u30af\u3092\u767a\u73fe\u3059\u308b\u30de\u30a6\u30b9\u3067\u3042\u308a\u3001\u9ad8\u30d3\u30aa\u30c1\u30f3\u98df\u6295\u4e0e\u306b\u3088\u308a\u8133\u3001\u5fc3\u81d3\u3001\u809d\u81d3\u3001\u7cbe\u5de3\u7d44\u7e54\u3067 BMAL1 \u8fd1\u4f4d\u30bf\u30f3\u30d1\u30af\u306e\u30d3\u30aa\u30c1\u30f3\u5316\u304c\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u3044\u308b\u3002<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n

\u8fd1\u63a5\u4f9d\u5b58\u6027\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u5316\u6cd5(BioID)\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8fd1\u508d\u306b\u5b58\u5728\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u3057\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u3092\u89e3\u6790\u3059\u308b\u6280\u8853\u3067\u3059\u3002\u7269\u7406\u7684\u306b\u76f4\u63a5\u76f8\u4e92\u4f5c\u7528\u3057\u3066\u3044\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u691c\u51fa\u3059\u308b\u5f93\u6765\u306e\u514d\u75ab\u6c88\u964d\u6cd5\u3001\u30d7\u30eb\u30c0\u30a6\u30f3\u30a2\u30c3\u30bb\u30a4\u6cd5\u3001\u30bf\u30f3\u30c7\u30e0\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u7cbe\u88fd\u7b49\u3068\u306f\u7570\u306a\u308a\u3001BioID\u6cd5\u306f\u3001\u3053\u308c\u307e\u3067\u691c\u51fa\u56f0\u96e3\u3067\u3042\u3063\u305f\u4e00\u904e\u6027\u3067\u5f31\u3044\u7d50\u5408\u56e0\u5b50\u3084\u96e3\u6eb6\u6027\u8907\u5408\u4f53\u306e\u8907\u5408\u56e0\u5b50\u306a\u3069\u3001\u8fd1\u508d\u306e\u95a2\u9023\u30bf\u30f3\u30d1\u30af\u8cea\u3082\u7db2\u7f85\u7684\u306b\u89e3\u6790\u53ef\u80fd\u3067\u3059\u3002\u5bc4\u8a17\u8005\u306e\u6751\u7530\u5148\u751f\u3089\u306f\u3001R118G\u5909\u7570\u3092\u6301\u3064BirA\u30d3\u30aa\u30c1\u30f3\u30ea\u30ac\u30fc\u30bc(BirA*)\u3092\u63a1\u7528\u3057\u3001Brain and Muscle ARNT1-Like 1 (BMAL1)\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3057\u305f\u3001\u30de\u30a6\u30b9\u500b\u4f53\u3067BioID\u53ef\u80fd\u306a\u30ce\u30c3\u30af\u30a4\u30f3\u30de\u30a6\u30b9\u3092\u4f5c\u88fd\u3057\u307e\u3057\u305f[1]\u3002
Bmal1\u306f\u6642\u8a08\u907a\u4f1d\u5b50\u3068\u3057\u3066\u77e5\u3089\u308c\u3001\u6838\u5185\u3067BMAL1\u30bf\u30f3\u30d1\u30af\u8cea\u3068CLOCK\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30d8\u30c6\u30ed2\u91cf\u4f53\u3092\u5f62\u6210\u3057\u3066\u3001\u30b5\u30fc\u30ab\u30c7\u30a3\u30a2\u30f3\u30ea\u30ba\u30e0\u306b\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u306e\u8ee2\u5199\u3092\u6d3b\u6027\u5316\u3057\u307e\u3059\u3002EGFPflox<\/sup>-BMAL1-BioID (RBRC12179)\u7cfb\u7d71\u306f\u3001Cre\u9175\u7d20\u975e\u5b58\u5728\u4e0b\u3067\u306f\u7dd1\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8ceaEGFP\u3092\u767a\u73fe\u3057\u3001Cre\u9175\u7d20\u306b\u3088\u308b\u7d44\u307f\u63db\u3048\u5f8c\u306b\u306fBMAL1-BirA*\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u767a\u73fe\u3057\u307e\u3059\u3002BMAL1-BioID (RBRC12180)\u7cfb\u7d71\u306f\u3001\u5185\u5728\u6027\u306eROSA26\u907a\u4f1d\u5b50\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u5236\u5fa1\u4e0b\u304b\u3064\u5916\u6765\u6027\u306eCAG\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u5236\u5fa1\u4e0b\u3067BMAL1-BirA*-HA\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u767a\u73fe\u3057\u307e\u3059\u3002\u5b9f\u969b\u306b\u672c\u7cfb\u7d71\u304b\u3089\u30de\u30a6\u30b9\u80ce\u5150\u7dda\u7dad\u82bd\u7d30\u80de(MEF)\u3092\u4f5c\u88fd\u3057\u3001\u4e88\u6e2c\u901a\u308a\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u304c\u8d77\u3053\u3063\u305f\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u751f\u4f53\u306b\u304a\u3044\u3066\u306f\u3001\u9ad8\u30d3\u30aa\u30c1\u30f3\u98df\u3092\u7d66\u990c\u3059\u308b\u3053\u3068\u3067\u3001\u975e\u4fb5\u8972\u7684\u306b\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u3044\u307e\u3059\u3002\u672c\u7cfb\u7d71\u306fBMAL1\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u76f8\u4e92\u4f5c\u7528\u56e0\u5b50\u306e\u89e3\u6790\u306b\u6709\u7528\u3067\u3059\u3002\u307e\u305f\u3001in vivo<\/i> BioID\u30b7\u30b9\u30c6\u30e0\u306f\u3055\u307e\u3056\u307e\u306a\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u5fdc\u7528\u53ef\u80fd\u3067\u3042\u308a\u3001\u4eca\u5f8c\u306e\u767a\u5c55\u5229\u7528\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002\n <\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n
Keywords<\/td>\n:<\/td>\nin vivo<\/i> BioID\u3001\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u76f8\u4e92\u4f5c\u7528\u3001\u30b5\u30fc\u30ab\u30c7\u30a3\u30a2\u30f3\u30ea\u30ba\u30e0\u3001\u9ad8\u30d3\u30aa\u30c1\u30f3\u98df<\/td>\n<\/tr>\n
Depositor<\/td>\n:<\/td>\n\u6751\u7530\u77e5\u5f25\u5148\u751f(\u7b51\u6ce2\u5927\u5b66\u30fb\u5bc4\u8a17\u5f53\u6642)<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nC57BL\/6J-Gt(ROSA)26Sor<em1(floxed-EGFP-Arntl-BirA<*>)Murk><\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC12179<\/td>\n<\/tr>\n
Strain name<\/td>\n:<\/td>\nC57BL\/6J-Gt(ROSA)26Sor<em1.1(floxed-EGFP-Arntl-BirA<*>)Murk><\/td>\n<\/tr>\n
RBRC No.<\/td>\n:<\/td>\nRBRC12180<\/td>\n<\/tr>\n
Reference<\/td>\n:<\/td>\n[1]<\/td>\nMurata K, Mimura A, Suzuki H, Mikami N, Hamada Y, Kato K, Iki N, Ishida M, Daitoku Y, Tanimoto Y, Okiyoneda T, Fujiyama T, Dinh TTH, Mizuno S, Sugiyama F.
\nEfficient induction of proximity-dependent labelling by biotin feeding in BMAL1-BioID knock-in mice.
\nJ Biochem.<\/em> 2021 Dec 4;170(4):453-461.\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n
December 2024
\nSaori Mizuno, Ph.D.
\nContact: Experimental Animal Division, RIKEN BioResource Research Center (animal.brc@riken.jp)<\/a>
\nAll materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":19,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"https:\/\/mus.brc.riken.jp\/ja\/wp-content\/uploads\/2024\/12\/MMfig_202412.jpg","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"https:\/\/mus.brc.riken.jp\/ja\/wp-content\/uploads\/2024\/12\/MMfig_202412.jpg","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-20434","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/20434","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=20434"}],"version-history":[{"count":1,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/20434\/revisions"}],"predecessor-version":[{"id":20435,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/20434\/revisions\/20435"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=20434"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}