{"id":5218,"date":"2011-07-18T16:03:57","date_gmt":"2011-07-18T07:03:57","guid":{"rendered":"http:\/\/mus.brc.riken.jp:7301\/?page_id=5218"},"modified":"2021-01-29T14:21:43","modified_gmt":"2021-01-29T05:21:43","slug":"jul_2011","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/current_tech\/jul_2011","title":{"rendered":"BRC Current Technology July 2011"},"content":{"rendered":"<table style=\"max-width:700px;border:0px;\">\n<tbody>\n<tr>\n<td style=\"text-align: center; white-space: nowrap; position:absolute;border:0px;background-color:#fff;\"><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/2013\/05\/brc_logo2.png\" alt=\"RIKEN BRC\"\/><\/td>\n<td style=\"text-align:center;border:0px;background-color:#fff;\"><a href=\"\/ja\/current_tech\" style=\"text-decoration: none;\"><span style=\"color:#000000; font-size: 25pt; line-height: 130%; font-weight: bold;\">BRC Current Technology<\/span><br \/>\n<span style=\"color:#0000ff; font-size: 25pt; line-height: 130%; font-weight: bold;\">July 2011<\/span><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;border:0px;background-color:#fff;\" colspan=\"2\">\n<h1 style=\"color: #0000ff;\">1. High Osmolality Vitrification (HOV)<\/h1>\n<h2>\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u6e29\u5ea6\u8f38\u9001\u3092\u53ef\u80fd\u306b\u3057\u305f\u80da\u30ac\u30e9\u30b9\u5316\u4fdd\u5b58\u6cd5<\/h2>\n<div style=\"text-align:center;\">\n<a href=\"\/ja\/wp-content\/uploads\/2013\/02\/current_tech1107_0101.png\"><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/2013\/02\/current_tech1107_0101.png\" alt=\"transportation of embryos in dry ice packages\" width=\"500\" height=\"\" \/><\/a><br \/>\n<a href=\"\/ja\/wp-content\/uploads\/2013\/02\/current_tech1107_0102.png\"><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/2013\/02\/current_tech1107_0102.png\" alt=\"FIMRe\u6a5f\u95a2\u3078\u306e\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u5bb9\u5668\u306b\u3088\u308b\u8f38\u9001\u5b9f\u9a13\" width=\"500\" height=\"\" \/><\/a><\/p>\n<\/div>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"border:0px;background-color:#fff; white-space: normal; text-indent: 1em;\" colspan=\"2\">RIKEN BRC \u3067\u306f\u3001\u5e74\u9593\uff11\uff10\uff10\u7cfb\u7d71\u4ee5\u4e0a\u306e\u30de\u30a6\u30b9\u3092\u51cd\u7d50\u80da\u3067\u63d0\u4f9b\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u51cd\u7d50\u80da\u8f38\u9001\u306b\u306f\u3001\u30c9\u30e9\u30a4\u30b7\u30c3\u30d1\u30fc\u3068\u547c\u3070\u308c\u308b\u7279\u6b8a\u306a\u5bb9\u5668\u304c\u4f7f\u308f\u308c\u307e\u3059\u3002\u30c9\u30e9\u30a4\u30b7\u30c3\u30d1\u30fc\u5185\u306b\u306f\u3001\u5438\u53ce\u5264\u304c\u57cb\u3081\u8fbc\u307e\u308c\u3066\u304a\u308a\u3001\u305d\u3053\u306b\u6db2\u4f53\u7a92\u7d20\u304c\u5438\u53ce\u3055\u308c\u308b\u3053\u3068\u3067\u3001-190\u2103\u4ee5\u4e0b\u3068\u3044\u3046\u8d85\u4f4e\u6e29\u3067\u5b89\u5168\u306b\u51cd\u7d50\u80da\u304c\u8f38\u9001\u3067\u304d\u307e\u3059\u3002 \u3057\u304b\u3057\u30c9\u30e9\u30a4\u30b7\u30c3\u30d1\u30fc\u306f\u91cd\u304f\u3001\u9ad8\u4fa1\u3067\u3042\u308a\u3001\u3057\u304b\u3082\u305d\u306e\u8f38\u9001\u306b\u306f\u5f80\u5fa9\u5206\u3092\u652f\u6255\u308f\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002\u3082\u3061\u308d\u3093\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u8a70\u3081\u8f38\u9001\u304c\u3067\u304d\u308c\u3070\u826f\u3044\u306e\u3067\u3059\u304c\u3001\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u6e29\u5ea6(-80\u2103)\u3067\u306f\u3001\u901a\u5e38\u306e\u30ac\u30e9\u30b9\u5316\uff08vitrification\uff09\u4fdd\u5b58\u80da\u306f\u8131\u30ac\u30e9\u30b9\u5316\u306b\u3088\u308b\u7d30\u80de\u5185\u6c37\u6676\u306e\u5f62\u6210\u306b\u3088\u308a\u6b7b\u6ec5\u3057\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u305d\u3053\u3067BRC\u3067\u306f\u3001\u9ad8\u77e5\u5927\u5b66\u3068\u306e\u5171\u540c\u3067\u3001\u901a\u5e38\u306e\u30ac\u30e9\u30b9\u5316\u4fdd\u5b58\u6db2(EFS40)\u306e\u6d78\u900f\u5727\u3092\u9ad8\u3081\u308b\u3053\u3068\u306b\u3088\u308a\u3001-80\u2103\u306b\u304a\u3044\u3066\u3082\u6570\u65e5\u9593\u30ac\u30e9\u30b9\u5316\u304c\u4fdd\u6301\u3067\u304d\u308b\u4fdd\u5b58\u6db2\u3092\u958b\u767a\u3057\u307e\u3057\u305f\uff08Jin et al., 2010\uff09\u3002 \u6700\u8fd1\u3001\u3055\u3089\u306b\u6539\u826f\u3092\u52a0\u3048\u3001\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u5185\u3067\u6570\u65e5\uff5e\u6570\u30f6\u6708\u306e-80\u2103\u4fdd\u5b58\u304c\u53ef\u80fd\u306a\u4fdd\u5b58\u6db2EFS45c\u306e\u958b\u767a\u306b\u6210\u529f\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u4fdd\u5b58\u6db2\u3092\u5229\u7528\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001 C57BL\/6 \u80da\u3092\u56fd\u5185\u306f\u3082\u3068\u3088\u308a\u3001\u6d77\u5916FIMRe \u6a5f\u95a2\u3067\u3042\u308bMRC (U.K.) \u304a\u3088\u3073UC Davis (U.S.A.) \u3078\u3082\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u5bb9\u5668\u3092\u7528\u3044\u3066\u80da\u3092\u8f38\u9001\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u307e\u3057\u305f\uff08\u4e0a\u8a18\u30c7\u30fc\u30bf\uff09\u3002 \u5c06\u6765\u306e\u51cd\u7d50\u4fdd\u5b58\u80da\u8f38\u9001\u3001\u7279\u306b\u30de\u30a6\u30b9\u30d0\u30f3\u30af\u304b\u3089\u306e\u63d0\u4f9b\u696d\u52d9\u306b\u7528\u3044\u3089\u308c\u308b\u3053\u3068\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002\n<p>\u203b\u672c\u6280\u8853\u306f\u3001\u6587\u90e8\u79d1\u5b66\u7701NBRP\u57fa\u76e4\u6280\u8853\u6574\u5099\u30d7\u30ed\u30b0\u30e9\u30e0\u300c\u5b9f\u9a13\u52d5\u7269\u30de\u30a6\u30b9\u53ca\u3073\u30e9\u30c3\u30c8\u30ea\u30bd\u30fc\u30b9\u306e\u8f38\u9001\u30b7\u30b9\u30c6\u30e0\u306e\u958b\u767a\u300d\u4e8b\u696d\u3068\u3057\u3066\u3001\u9ad8\u77e5\u5927\u5b66\u3001\u653e\u5c04\u7dda\u533b\u5b66\u7dcf\u5408\u7814\u7a76\u6240\u3001 Medical Research Council (\u82f1\u56fd), University of California, Davis (\u7c73\u56fd) \u3068\u306e\u5171\u540c\u7814\u7a76\u306b\u3088\u308a\u958b\u767a\u3055\u308c\u307e\u3057\u305f\u3002\n<\/p><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"max-width:700px;\">\n<tbody>\n<tr>\n<td style=\"border:0px;background-color:#fff;\" valign=\"top\">Reference:<\/td>\n<td style=\"border:0px;background-color:#fff;\" valign=\"top\"><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19828776?dopt=Abstract\" target=\"_blank\" rel=\"noopener noreferrer\">Jin et al., Biol. Reprod. 82, 444-450 (2010)<\/a><br \/>\nMochida K &amp; Ogura A, (review) J. Mamm. Ova Res. 27, 87-92 (2010)<br \/>\n<\/a>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>BRC Current Technology July 2011 1. High Osmolality Vitrification (HOV) \u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u6e29\u5ea6\u8f38\u9001\u3092\u53ef\u80fd\u306b\u3057\u305f\u80da\u30ac\u30e9\u30b9\u5316\u4fdd\u5b58\u6cd5 RIKEN BRC \u3067\u306f\u3001\u5e74\u9593\uff11\uff10\uff10\u7cfb\u7d71\u4ee5\u4e0a\u306e\u30de\u30a6\u30b9\u3092\u51cd\u7d50\u80da\u3067\u63d0\u4f9b\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u51cd\u7d50\u80da\u8f38\u9001\u306b\u306f\u3001\u30c9\u30e9\u30a4\u30b7\u30c3\u30d1\u30fc\u3068\u547c\u3070\u308c\u308b\u7279\u6b8a\u306a\u5bb9\u5668\u304c\u4f7f\u308f\u308c\u307e\u3059\u3002\u30c9\u30e9\u30a4\u30b7\u30c3\u30d1\u30fc\u5185\u306b\u306f\u3001\u5438\u53ce\u5264\u304c\u57cb\u3081\u8fbc\u307e\u308c\u3066\u304a\u308a\u3001\u305d\u3053\u306b\u6db2 [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":3268,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-5218","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5218","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=5218"}],"version-history":[{"count":9,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5218\/revisions"}],"predecessor-version":[{"id":17910,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5218\/revisions\/17910"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3268"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=5218"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}