{"id":5249,"date":"2013-06-27T11:09:26","date_gmt":"2013-06-27T02:09:26","guid":{"rendered":"http:\/\/mus.brc.riken.jp:7301\/?page_id=5249"},"modified":"2021-04-07T14:03:14","modified_gmt":"2021-04-07T05:03:14","slug":"mar_2012_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/mar_2012_mm","title":{"rendered":"Mar 2012 Autophagy monitoring congenic strains"},"content":{"rendered":"

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Autophagy monitoring congenic strains<\/em><\/strong><\/h2>\n
B6.B6D2-Tg(CAG-GFP\/LC3)53Nmz\/NmzRbrc (GFP-LC3#53) RBRC00806 <\/a><\/h5>\n
C.B6D2-Tg(CAG-GFP\/LC3)53Nmz\/Rbrc RBRC01413 <\/a><\/h5>\n
C3.B6D2-Tg(CAG-GFP\/LC3)53Nmz\/Rbrc RBRC01414 <\/a><\/h5>\n
MSM.B6D2-Tg(CAG-GFP\/LC3)53Nmz\/Rbrc RBRC01227 <\/a><\/h5>\n
\"Autophagy
\"Autophagy<\/a>
(avi file:12074KB\/2s)<\/figcaption><\/figure>\n

A: B6.B6D2-Tg(CAG-GFP\/LC3)53NmzRbrc (RBRC00806), a donor strain in the C57BL\/6JJcl to generate other three congenic lines.
\nB: C.B6D2-Tg(CAG-GFP\/LC3)53NmzRbrc (RBRC01413) in the BALB\/cAnN.
\nC: C3.B6D2-Tg(CAG-GFP\/LC3)53NmzRbrc (RBRC01414) in the C3H\/HeNCrlj.
\nD: Live imaging of autophagosome in pancreatic tissues of B6-GFP-LC3 mice (RBRC00806). For imaging technology details, please refer Cao L et al. High Resolution Intravital Imaging of Subcellular Structures of Mouse Abdominal Organs Using a Microstage Device. PLoS ONE 2012.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Autophagy\u00a0(Greek for “self-eating”) is known to have important roles in various physiological and pathological processes such as maintenance of the amino acid pool during starvation, preimplantation development, prevention of neurodegeneration, anti-aging, tumor suppression, clearance of intracellular microbes, and regulation of innate and adaptive immunity [1,2]. Accordingly, there is a growing need among scientists to be able to detect autophagy\u00a0in vivo<\/em>. The GFP-LC3#53 transgenic mouse is one of the most widely used\u00a0in vivo<\/em>\u00a0autophagy monitoring tools and was developed by Dr. Noboru Mizushima [3]. This transgenic mouse enables the accurate detection of autophagosome in diverse biological processes. We have extended its capability to other useful inbred strains such as\u00a0BALB\/cAnN (RBRC00641)<\/a>, C3H\/HeNCrlj, and\u00a0MSM\/MsRbrc (RBRC00209)<\/a>\u00a0by successive backcrossing. The genetic backgrounds of these strains were examined using simple sequence length polymorphism markers to assess congenic status. A genotyping protocol was also established to distinguish homozygous and hemizygous transgenic mice. These autophagy monitoring mice will be useful for assessing autophagy in various mouse disease models.\n<\/div>\n

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What is autophagy?<\/td>\n:<\/td>\n\u00a0http:\/\/www.cellcycle.m.u-tokyo.ac.jp\/english\/research\/index.html<\/a><\/td>\n<\/tr>\n
Depositor of donor strain:<\/td>\n:<\/td>\n\u00a0Dr. Noboru Mizushima, Department of Biochemistry and Molecular Biology, The University of Tokyo<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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References<\/td>\n:<\/td>\n[1]<\/td>\nMizushima N, Komatsu M. Autophagy: renovation of cells and tissues.Cell 147(4):728-741, 2011.<\/a><\/td>\n<\/tr>\n
[2]<\/td>\nMizushima N, Yoshimori T, Levine B.Methods in mammalian autophagy research.Cell 140(3):313-326, 2010.<\/a><\/td>\n<\/tr>\n
[3]<\/td>\nMizushima N, Yamamoto A, Matsui M, Yoshimori T, Ohsumi Y. In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker.\u00a0Mol Biol Cell 15(3):1101-1111, 2004.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Autophagy monitoring congenic strains B6.B6D2-Tg(CAG-GFP\/LC3)53Nmz\/NmzRbrc (GFP-LC3#53) RBRC00806 C.B6D2-Tg(CAG-GFP\/LC3)53Nmz\/Rbrc RBRC01413 C3.B6D2-Tg(CAG-GFP\/LC3)53Nmz\/Rbrc RBRC01414 MSM.B6D2-Tg(CAG […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-5249","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5249","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=5249"}],"version-history":[{"count":20,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5249\/revisions"}],"predecessor-version":[{"id":18143,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5249\/revisions\/18143"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=5249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}