{"id":5268,"date":"2013-06-27T11:19:56","date_gmt":"2013-06-27T02:19:56","guid":{"rendered":"http:\/\/mus.brc.riken.jp:7301\/?page_id=5268"},"modified":"2021-04-07T13:57:38","modified_gmt":"2021-04-07T04:57:38","slug":"jun_2011_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/jun_2011_mm","title":{"rendered":"June 2011 A Dnmt1 knock-out mouse for epigenetic studies<\/em>"},"content":{"rendered":"

\"mouse_of_month_201106\"<\/a><\/p>\n\n\n\n
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A Dnmt1 knock-out mouse for epigenetic studies<\/em><\/h2>\n
129;B6-Dnmt1tm1Okan<\/sup><\/em>\u00a0(Acc. No. CDB0500K) RBRC04774<\/a><\/strong><\/h5>\n

\"mn1106_0101\"
\n(A) Side (top) and dorsal (bottom) view of\u00a0Dnmt1ps<\/sup><\/em>\u00a0mutant embryos at E9.5. (B-D) Cytological epigenetic defects in\u00a0Dnmt1ps<\/sup><\/em>\u00a0mutant cell nuclei. (B) Global loss of 5-methylcytosine (5mC). (C) Decrease of HP1\u03b2 association at heterochromatic regions intensely stained by DAPI. (D) Loss of hemi-methylated CpG-dependent DNMT1 localization at replication foci (PCNA).\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Epigenetic changes are defined as modifications that induce heritable changes in gene expression without changes in the DNA sequence. The methylation of cytosine on the 5-carbon position is a heritable epigenetic DNA modification that affects mammalian development. Three DNA methyltransferases, DNMT1, DNMT3A, and DNMT3B, are coordinately expressed and play an important role in the regulation of gene expression, genomic imprinting, and embryogenesis. 129;B6-Dnmt1tm1Okan<\/sup><\/em>\u00a0mice (Acc. No. CDB0500K, RBRC04774) were generated to examine the roles of the catalytic activity of DNMT1\u00a0in vivo<\/em>\u00a0by creating a\u00a0Dnmt1ps<\/sup><\/em>allele that expresses a protein with a point mutation (DNMT1-C1229S) that lacks catalytic activity. Homozygous\u00a0Dnmt1ps<\/sup><\/em>mutant mice exhibit developmental arrest, accompanied by multiple defects, shortly after gastrulation. The phenotypic similarity of the homozygous\u00a0Dnmt1ps<\/sup><\/em>mutant mice with DNMT1-null mutant (Dnmt1c<\/sup><\/span><\/em>) mice clearly indicates that the catalytic activity of DNMT1 is essential for mammalian development. The 129;B6-Dnmt1tm1Okan<\/sup><\/em>\u00a0mice together with other\u00a0Dnmt<\/em>\u00a0knock-out mice are invaluable strains to study the role of these methyltransferases in mammalian epigenetics.<\/strong>\n<\/div>\n

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Other related strains<\/td>\n:<\/td>\nB6;129S4-Dnmt1tm2Enl<\/sup><\/em>\u00a0RBRC01737<\/a>
\nB6;129S4-Dnmt3atm1Enl<\/sup><\/em>\u00a0RBRC03730<\/a>
\nB6;129S4-Dnmt3atm3.1Enl<\/sup><\/em>\u00a0RBRC03731<\/a>
\nB6;129S4-Dnmt3btm1Enl<\/sup><\/em>\u00a0RBRC03732<\/a>
\nB6;129S4-Dnmt3btm5.1Enl<\/sup><\/em>\u00a0RBRC03733<\/a><\/td>\n<\/tr>\n
Depositor<\/td>\n:<\/td>\nDr. Masaki Okano, RIKEN Center for Development Biology<\/td>\n<\/tr>\n
Reference<\/td>\n:<\/td>\n Takebayashi S, Tamura T, Matsuoka C, Okano M. Major and essential role for the DNA methylation mark in mouse embryogenesis and stable association of DNMT1 with newly replicated regions.\u00a0Mol Cell Biol.<\/em>\u00a02007 27(23):8243-58<\/a>.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

A Dnmt1 knock-out mouse for epigenetic studies 129;B6-Dnmt1tm1Okan\u00a0(Acc. No. CDB0500K) RBRC04774 (A) Side (top) and dorsal (bottom) view of\u00a0Dnmt1ps\u00a0mutant embryos at E9.5. (B-D) Cytological epigenetic […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-5268","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5268","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=5268"}],"version-history":[{"count":7,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5268\/revisions"}],"predecessor-version":[{"id":18129,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/5268\/revisions\/18129"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=5268"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}