{"id":6402,"date":"2009-06-30T13:43:04","date_gmt":"2009-06-30T04:43:04","guid":{"rendered":"http:\/\/mus.brc.riken.jp:7301\/?page_id=6402"},"modified":"2025-02-12T15:44:39","modified_gmt":"2025-02-12T06:44:39","slug":"jun_2009_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/jun_2009_mm","title":{"rendered":"Jun 2009 C57BL\/6-Ly5 Antigen (Ptprc) Congenic Strain A cell surface marker molecule for bone marrow transplantation"},"content":{"rendered":"

\"mouse_of_month_200906\"<\/a><\/p>\n\n\n\n
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C57BL\/6-Ly5 Antigen (Ptprc<\/em>) Congenic Strain
\nA cell surface marker molecule for bone marrow transplantation<\/strong><\/h2>\n
B6.SJL-Ptprca<\/sup><\/em>\/BoyYuoRbrc (C57BL\/6-Ly5a<\/sup><\/em>, C57BL\/6-Lya.1) RBRC00126<\/h5>\n

\"mn0906_0101\"\"mn0906_0102\"
\nFig. 1 (A) The C57BL\/6-Ly5.1 congenic mouse. (B) A flow cytometry analysis of peripheral blood cells can be used to distinguish donor cells of the C57BL\/6-Ly5.1 congenic mouse from cells of the C57BL\/6 (Ly5.2) recipient mouse with fluorescence labeled anti-Ly5.1\/CD45.1 antibody after bone marrow transplantation experiment. The data was kindly provided by Dr. Hiroyuki Miyoshi, Subteam of manipulation of cell fate, RIKEN BRC.\u00a0Please see also Fig 2 \u201cComparative repolulation experiment\u201d<\/a>.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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An useful cell marker system for hematopoietic cells in transplantation studies<\/em><\/strong>
\nThe protein-tyrosine phosphatase, receptor type, C (Ptprc<\/em>), also known as\u00a0Ly5<\/em>\u00a0or\u00a0Cd45<\/em>\u00a0gene of the mouse, encodes a set of transmembrane glycoprotein isoforms expressed only on hematopoietic cells [1, 2].\u00a0Ptprc<\/em>\u00a0was found to express in multiple forms by alternative splicing of different exons [3], and in allelic forms designated as Ly5.1\/Cd45.1 of SJL\/J and Ly5.2\/Cd45.2\/ of C57BL\/6J [4, 5]. A Congenic strain, B6.SJL-Ptprca<\/sup><\/em>\/Boy has been generated by backcrossing SJL\/J carrying\u00a0Ptprca<\/sup><\/em>\u00a0(Ly5a<\/sup><\/em>\/Cd45a<\/sup><\/em>, Ly5.1\/Cd45.1) to the C57BL\/6 background strain for hematological studies [6]. RIKEN BRC distributes B6.SJL-Ptprca<\/sup><\/em>\/BoyYuoRbrc (C57BL\/6-Ly5a<\/sup><\/em>, C57BL\/6-Ly5.1, RBRC00126) mice (Fig. 1) as a useful cell marker system in bone marrow transplantation studies. The specific mouse monoclonal antibodies for the Cd45.1 and Cd45.2 have been exploited [4] and widely used to distinguish hematopoitetic cells of donor and recipient mice. A typical transplantation experiment is shown in\u00a0Fig. 2<\/a>.\n<\/div>\n

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Confusing Ly5 gene nomenclature and its history<\/em><\/strong>
\nRevision of gene and allele names for\u00a0Ptprc<\/em>\u00a0(Ly5\/Cd45<\/em>) have led to much confusion regarding antigen specificity and allelic symbols [7]. Present and past nomenclature of\u00a0Ly5<\/em>\u00a0gene is summarized in Table 1.
\nKomura,\u00a0et al.<\/em>\u00a0(1975), described an antigen expressed on T cells, and designated the specificity on C57BL\/6 cells as Ly5.1 and on SJL cells as Ly5.2 [1]. According to gene naming convention for alleles, the C57BL\/6 allele became\u00a0Ly5a<\/sup><\/em>\u00a0and SJL\/J allele became\u00a0Ly5b<\/sup><\/em>.
\nIn 1987, Morse\u00a0et al.<\/em>\u00a0proposed a revision of nomenclature of mouse lymphocyte antigens, which led to a reversed designation [8]. Consequently, the B6 allele became\u00a0Ly5b<\/sup><\/em>\u00a0(antigen specificity Ly5.2) and the SJL allele became\u00a0Ly5a<\/sup><\/em>\u00a0(antigen specificity Ly5.1).
\nA further revision was made on the cell surface antigens by Morse in 1992, where Ly5 became\u00a0Cd45<\/em>[9].
\nFinally, the gene name was changed to Ptprc in 1993, based on the work by Charbonneau\u00a0et al.<\/em>\u00a0who demonstrated homology between a tyrosine phosphatase and CD45 protein [2]. This led to the present nomenclature of the B6 as\u00a0Ptprcb<\/sup><\/em>\u00a0and the SJL as\u00a0Ptprca<\/sup><\/em>.\n<\/div>\n

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Depositor<\/td>\n:<\/td>\nDr. Toshitada Takahashi, Aichi Cancer Center Research Institute<\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\n\u00a01.<\/td>\nKomura K, Itakura K, Boyse EA, John M. Ly-5: a new lymphocyte antigen system.Immunogenetics.\u00a0<\/em>1975 1:452-456.<\/td>\n<\/tr>\n
\u00a02.<\/td>\nCharbonneau H, Tonks NK, Walsh KA, Fischer EH. The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase.\u00a0Proc Natl Acad Sci U S A.<\/strong><\/em>1988 Oct;85(19):7182-6.<\/a><\/td>\n<\/tr>\n
\u00a03.<\/td>\nSaga Y, Tung JS, Shen FW, and Boyse EA. Sequences of Ly-5 cDNA: isoform-related diversity of Ly-5 mRNA.\u00a0PNAS USA.\u00a0<\/strong><\/em>1986 83: 6940-6944.<\/a><\/td>\n<\/tr>\n
\u00a04.<\/td>\nShen FW, Tung JS, and Boyse EA. Further definition of the Ly-5 system.Immunogenetics.\u00a0<\/strong><\/em>1986 24: 146-149.<\/a><\/td>\n<\/tr>\n
\u00a05.<\/td>\nTung JS, Scheid MP, Pierotti MA, Hammerling U, and Boyse EA. Structural features and selective expression of three Ly-5+ cell-surface molecules.\u00a0Immunogenetics.\u00a0<\/strong><\/em>1981 14: 101-106.<\/a><\/td>\n<\/tr>\n
\u00a06.<\/td>\nShen FW, Saga Y, Litman G, Freeman G, Tung JS, Cantor H, and Boyse EA. Cloning of Ly-5 cDNA.\u00a0PNAS USA.\u00a0<\/strong><\/em>1985 82: 7360-7363.<\/a><\/td>\n<\/tr>\n
\u00a07.<\/td>\nMobraaten LE.\u00a0Ly5<\/em>\u00a0gene nomenclature, C57BL\/6J and SJL\/J – A history of change.JAX Notes.\u00a0<\/strong><\/em>1994 458, 2.<\/td>\n<\/tr>\n
\u00a08.<\/td>\nMorse HC III, Shen FW, Hammerling U. Genetic nomenclature for loci controlling mouse lymphocyte antigens.\u00a0Immunogenetics.\u00a0<\/strong><\/em>1987 25:71-78.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

C57BL\/6-Ly5 Antigen (Ptprc) Congenic Strain A cell surface marker molecule for bone marrow transplantation B6.SJL-Ptprca\/BoyYuoRbrc (C57BL\/6-Ly5a, C57BL\/6-Lya.1) RBRC00126 Fig. 1 (A) The C57BL\/6-Ly5.1 […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6402","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6402","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6402"}],"version-history":[{"count":10,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6402\/revisions"}],"predecessor-version":[{"id":20521,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6402\/revisions\/20521"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6402"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}