{"id":6411,"date":"2009-05-30T14:10:14","date_gmt":"2009-05-30T05:10:14","guid":{"rendered":"http:\/\/mus.brc.riken.jp:7301\/?page_id=6411"},"modified":"2021-04-07T14:13:08","modified_gmt":"2021-04-07T05:13:08","slug":"may_2009_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/may_2009_mm","title":{"rendered":"May 2009 The only one strain for study of NF-kB\/RelA subunit in vivo<\/em>"},"content":{"rendered":"

\"mouse_of_month_200905\"<\/a><\/p>\n\n\n\n\n
\n

The only one strain for study of NF-kB\/RelA subunit in vivo<\/em><\/strong><\/h2>\n
TNF\u03baB RBRC 00305<\/a><\/h5>\n<\/td>\n<\/tr>\n

\"mn0905_0101\"(1)Body weight change:<\/strong>
\nThere is the significant growth retardation in TNF-kB mice (genotype; TNF-\/- RelA-\/-). The body weight of TNF-kB mice at 3 weeks after birth is significantly low, as shown in figure-1.<\/td>\n		\"mn0905_0102\"(2)TNF sensitivity of macrophages:<\/strong>
\nTNF-kB mice (genotype: TNF-\/- RelA-\/-)-derived macrophages are highly sensitive to TNF, as shown in figure-2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u00a0\u00a0TNF, tumor necrosis factor is a multifunction proinflammatory cytokine. RelA, v-rel reticuloendotheliosis viral oncogene homolog A (Avian) is one of NF-\u03baB family relating to the development of lymphocytes. Mice lacking only\u00a0Rela<\/em>\u00a0gene die between days 14 and 15 of embryogenesis because of liver cell apoptosis by endogenous TNF cytotoxicity. Meanwhile, homozyogous\u00a0Tnf\/Rela<\/em>\u00a0double-deficient mice are viable and have normal liver at birth. This strain is only one which is useful for studies of functions of\u00a0Rela in vivo<\/em>. Neomycin casettes were inserted into the nucleotides 3704-5364 of the\u00a0Tnf<\/em>\u00a0gene and the downstream of the translation initiation codon of the\u00a0Rela<\/em>\u00a0gene, respectively. Homozygous TNF-kB deficient mice are born with normal livers, but die within 5 weeks after birth with growth retardation as shown in figure-1. And the macrophages derived from this strain are highly sensitive to TNF, as shown in figure-2.\n<\/div>\n

 <\/p>\n\n\n\n\n\n\n
Depositor<\/td>\n:<\/td>\nTakahiro DOI, RIKEN BRC<\/a><\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\n1.<\/td>\nDoi TS\u00a0et al.<\/em>\u00a0NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.\u00a0J Exp Med.\u00a0<\/strong><\/em>1997 Mar 3;185(5):953-961.<\/a><\/td>\n<\/tr>\n
2.<\/td>\nMarino MW\u00a0et al.\u00a0<\/em>Characterization of tumor necrosis factor-deficient mice.\u00a0Proc Natl Acad Sci U S A.\u00a0<\/strong><\/em>1997 Jul 22;94(15):8093-8098.<\/a><\/td>\n<\/tr>\n
3.<\/td>\nDoi TS\u00a0et al.\u00a0<\/em>Absence of tumor necrosis factor rescues RelA-deficient mice from embryonic lethality.\u00a0Proc Natl Acad Sci U S A.\u00a0<\/strong><\/em>1999 Mar 16;96(6):2994-2999.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

The only one strain for study of NF-kB\/RelA subunit in vivo TNF\u03baB RBRC 00305 (1)Body weight change: There is the significant growth retardation in TNF-kB mice (genotype; TNF-\/- RelA-\/-). The body weig […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6411","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6411","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6411"}],"version-history":[{"count":8,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6411\/revisions"}],"predecessor-version":[{"id":18155,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6411\/revisions\/18155"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6411"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}