{"id":6432,"date":"2009-02-10T15:24:45","date_gmt":"2009-02-10T06:24:45","guid":{"rendered":"http:\/\/mus.brc.riken.jp:7301\/?page_id=6432"},"modified":"2021-04-07T13:38:23","modified_gmt":"2021-04-07T04:38:23","slug":"feb_2009_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/feb_2009_mm","title":{"rendered":"Feb 2009 The Tsix\/Xist-deficient Strains<\/em> Models for Epigenetics Study"},"content":{"rendered":"

\"mouse_of_month_200902\"<\/a><\/p>\n\n\n\n
\n

The Tsix\/Xist-deficient Strains<\/em>
\nModels for Epigenetics Study<\/h2>\n
B6.Cg-Xisttm2Sado<\/sup>\u00a0(Xist1loxGFP<\/sup>)<\/em> RBRC 01260<\/a><\/h5>\n
B6.Cg-Tsixtm1Sado<\/sup>\u00a0(TsixdeltaSA<\/sup>)<\/em> RBRC 01949<\/a><\/h5>\n
B6.Cg-Xisttm1Sado<\/sup>Tsixtm1Enl<\/sup>\u00a0(Xist1lox<\/sup>\u00a0TsixAA2delta1.7<\/sup>)<\/em> RBRC 01950<\/a><\/h5>\n
B6.Cg-Tsix\/Xisttm3Sado<\/sup>\u00a0(XistpA<\/sup>)<\/em> RBRC 02653<\/a><\/h5>\n
B6.Cg-Xisttm4Sado<\/sup>\u00a0(XistIVS19<\/sup>)<\/em> RBRC 02654<\/a><\/h5>\n
B6.Cg-Xisttm5Sado<\/sup>\u00a0(XistdeltaA<\/sup>)<\/em> RBRC 02655<\/a><\/h5>\n
Tsix\u00a0<\/em>: X (inactive)-specific transcript, antisense gene on Chr X<\/strong>
\nXist\u00a0<\/em>: inactive X specific transcripts gene on Chr X<\/strong><\/h5>\n

\"mn0902_0101\"<\/p>\n

Xist1loxGFP<\/sup><\/em>\u00a0mice RBRC 01260<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n
\"mn0902_0102\"<\/td>\n<\/td>\n\"mn0902_0103\"<\/td>\n\u00a0<\/strong><\/td>\nIn mammals, females have twice as many copies of genes on X chromosome as males do and develop a mechanism to equalize dosage of X-linked gene products by inactivating one of two X chromosomes (Lyon, 1961).
\nThe X-inactivation, also known as Lyonization, is regulated by the noncoding Xist<\/em> gene and its antisense non-coding Tsix<\/em> gene on Chr X (Sado et al<\/em>., 2001).
\nThe targeted disruption of the Xist<\/em> gene demonstrated that the expression of the Xist<\/em> gene is essential to initiate inactivating X chromosome in cis<\/em> (Marahrens et al., 1997; Penny et al., 1996).
\nThe expression of the Xist<\/em> gene was shown to be suppressed by the Tsix<\/em> gene through modifications of chromatin structure (Sado et al<\/em>., 2005).
\nThese strains provide unique and useful models to study the mechanism of X-inactivation in mammals and biological functions of noncoding RNA (Table 1<\/a>).<\/td>\n<\/tr>\n
(\u2191) Female embryos carrying mutated Xist1lox<\/sup><\/em> allele derived from the father died soon after implantation due to a dysplasia of extra-embryonic tissues. (\u2190) Normal embryos. scale bar = 200\u03bcm.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n
Depositor<\/td>\n:<\/td>\n\u00a0Dr. Takashi Sado, Division of Human Genetics, National Institute of Genetics<\/a><\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\n[1]<\/td>\n\u00a0Lyon M.F. 1961. Gene action in the X-chromosome of the mouse (Mus musculus<\/em>\u00a0L.).
\nNature<\/em>\u00a0190: 372-373.<\/a><\/td>\n<\/tr>\n
[2]<\/td>\nMarahrens Y., Panning B., Dausman J., Strauss W., Jaenisch R., 1997.\u00a0Xist<\/em>-deficient mice are defective in dosage compensation but not spermatogenesis.Genes Dev 11 (2): 156-166.<\/a><\/td>\n<\/tr>\n
[3]<\/td>\nPenny G.D., Kay G.F., Sheardown S.A., Rastan S., Brockdorff N. 1996. Requirement for\u00a0Xist<\/em>in X chromosome inactivation. Nature 379 (6561): 131-137.<\/a><\/td>\n<\/tr>\n
[4]<\/td>\nSado, T., Hoki, Y., and Sasaki, H. 2005.\u00a0Tsix<\/em>\u00a0silences\u00a0Xist<\/em>\u00a0through modification of chromatin structure.\u00a0Dev Cell<\/em>\u00a09: 159-165.<\/a><\/td>\n<\/tr>\n
[5]<\/td>\nSado T., Wang Z., Sasaki H., Li E. 2001. Regulation of imprinted X-chromosome inactivation in mice by\u00a0Tsix<\/em>.\u00a0Development<\/em>\u00a0128 (8): 1275-1286.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

The Tsix\/Xist-deficient Strains Models for Epigenetics Study B6.Cg-Xisttm2Sado\u00a0(Xist1loxGFP) RBRC 01260 B6.Cg-Tsixtm1Sado\u00a0(TsixdeltaSA) RBRC 01949 B6.Cg-Xisttm1SadoTsixtm1Enl\u00a0(Xist1lox\u00a0TsixAA2delta1.7 […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6432","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6432","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6432"}],"version-history":[{"count":9,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6432\/revisions"}],"predecessor-version":[{"id":18093,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6432\/revisions\/18093"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6432"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}