{"id":6524,"date":"2007-12-31T14:53:57","date_gmt":"2007-12-31T05:53:57","guid":{"rendered":"http:\/\/mus.brc.riken.jp:7301\/?page_id=6524"},"modified":"2022-07-12T11:02:11","modified_gmt":"2022-07-12T02:02:11","slug":"dec_2007_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/dec_2007_mm","title":{"rendered":"Nanog-GFP Transgenic Mouse"},"content":{"rendered":"\n\n\n
\"RIKEN<\/td>\nDecember 2007<\/span>
\nMouse of the Month<\/span><\/a>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n
\n

Nanog-GFP Transgenic Mouse
\nA tool for generation of germline-competent iPS cells<\/h2>\n

BRC No. 02290_STOCK Tg(Nanog-GFP,Puro)1Yam<\/a><\/h5>\n<\/td>\n<\/tr>\n
Research Application<\/td>\n:<\/td>\nStem Cell Research
\nDevelopmental Biology<\/td>\n<\/tr>\n
\"mn0712_0101\"
\nGeneration of iPS cells by using Nanog-GFP Tg mouse (Nature, 448, 313-317 (2007)<\/a>)<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n
\"mn0712_0103\"<\/td>\n\"mn0712_0102\"<\/td>\n<\/tr>\n
Germline-competent iPS cells from Nanog-GFP transgenic mouse.<\/strong>
\nPhase contrast (left) and fluorescence (right) micrographs are shown.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n<\/div>\n
\u00a0\u00a0Nanog gene is a good candidate marker for maintaining pluripotent differentiation, because of the loss of pluripotent epiblast in Nanog KO mice, the tendency of Nanog-null ES cells to differenciate spontaneously, and the capability of Nanog-overexpressed ES cells to maintain self-renewal independently of leukaemia inhibitory factor (LIF). Nanog-GFP transgene was generated by insertion of GFP-IRES-puromycin resistance gene (Puror<\/sup>) cassette into the 5′ untranslated region of a bacterial artificial chromosome (BAC) containing the mouse\u00a0Nanog<\/i>\u00a0gene. ES cells with the Nanog-GFP transgene were positive for GFP, but became negative when differentiation was induced. Using this system, pluripotent stem cells competent for germline chimaeras can be induced from Nanog-GFP transgenic mouse fibroblasts by introduction of candidate genes for pluripotency.\n<\/div>\n
\n<\/div>\n
The iPS cells will be available soon from\u00a0RIKEN BRC Cell Bank<\/a>.\n<\/div>\n

 <\/p>\n\n\n\n\n
Depositor<\/td>\n:<\/td>\nDr. Shinya Yamanaka (Kyoto University)<\/td>\n<\/tr>\n
Reference<\/td>\n:<\/td>\n\u00a0Nature<\/i>\u00a0(2007) 448:313-7.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

December 2007 Mouse of the Month   Nanog-GFP Transgenic Mouse A tool for generation of germline-competent iPS cells BRC No. 02290_STOCK Tg(Nanog-GFP,Puro)1Yam Research Application : Stem Cell Res […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6524","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6524","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6524"}],"version-history":[{"count":10,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6524\/revisions"}],"predecessor-version":[{"id":19141,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6524\/revisions\/19141"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6524"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}