{"id":6594,"date":"2007-02-01T10:37:37","date_gmt":"2007-02-01T01:37:37","guid":{"rendered":"http:\/\/mus.brc.riken.jp:7301\/?page_id=6594"},"modified":"2025-02-12T15:40:24","modified_gmt":"2025-02-12T06:40:24","slug":"feb_2007_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/feb_2007_mm","title":{"rendered":"FLPe transgenic mouse"},"content":{"rendered":"\n\n\n
\"RIKEN<\/td>\nFebruary 2007<\/span>
\nMouse of the Month<\/span><\/a>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n
\n FLPe transgenic mouse
\nA tool for conditional genetic manipulation<\/span><\/p>\n
BRC No. 01834_B6-Tg(CAG-FLPe)36<\/a>
\nBRC No. 01835_B6-Tg(CAG-FLPe)37<\/h5>\n<\/td>\n<\/tr>\n
\"Mouse
\nBRC No. 01834_B6-Tg(CAG-FLPe)36<\/td>\n		\"\"
\nFLP-FRT system:<\/strong> Floxed mice can be genera<\/span>ted after excision of neo (selective marker gene) by crossing with FLP Tg mice.<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u00a0 \u00a0 The FLP-FRT system is similar to the cre-loxP system and is useful for more complex dual approaches that combine FLP with other recombinases, such as Cre and \u03a6C31.. FLPe is a variant of the FLP recombinase with a higher enzymatic activity at 37\u00b0C. This transgenic mouse of C57BL\/6 strain expresses the FLPe recombinase under the control of the CAG promoter. This strain can be used as ‘FLP deleter’ mouse by crossing with the other genetically-engineered mice that have a pair of FLP recombinase target (FRT) sequence flanking the genomic region of interest.\n<\/div>\n

 <\/p>\n\n\n\n\n
Depositor<\/td>\n:<\/td>\nShigeyoshi Itohara (RIKEN BSI)<\/td>\n<\/tr>\n
Reference<\/td>\n:<\/td>\nKanki H, Suzuki H, Itohara S.
\nHigh-efficiency CAG-FLPe deleter mice in C57BL\/6J background.
\nExp. Anim.,<\/i>\u00a055, 137-141 (2006).<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

February 2007 Mouse of the Month FLPe transgenic mouse A tool for conditional genetic manipulation BRC No. 01834_B6-Tg(CAG-FLPe)36 BRC No. 01835_B6-Tg(CAG-FLPe)37 BRC No. 01834_B6-Tg(CAG-FLPe)36 FLP-F […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-6594","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6594","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=6594"}],"version-history":[{"count":14,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6594\/revisions"}],"predecessor-version":[{"id":20519,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/6594\/revisions\/20519"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=6594"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}