{"id":8465,"date":"2013-02-09T14:38:41","date_gmt":"2013-02-09T05:38:41","guid":{"rendered":"http:\/\/mus.brc.riken.jp:8001\/?page_id=8465"},"modified":"2021-04-07T13:39:02","modified_gmt":"2021-04-07T04:39:02","slug":"feb_2013_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/feb_2013_mm","title":{"rendered":"February&March 2013 Multifunctional Rosa26 reporter mouse strain"},"content":{"rendered":"

\"mouse_of_month_201302\"<\/a><\/p>\n\n\n\n
\n

Multifunctional Rosa26 reporter mouse strain<\/em><\/h2>\n

B6;129S6-Gt(ROSA)26Sor<tm1(CAG-mTFP1)Imayo>\/ImayoRbrc\u00a0\u00a0RBRC05146<\/a>
\n\"mn1302_0101\"<\/a><\/p>\n

Courtesy of Dr. Itaru Imayoshi, Kyoto University<\/p>\n

Schematic illustration of the Cre- and\/or Flp-dual recombinase responsive alleles.<\/strong>
\nA transgene cassette composed of an SA (splice acceptor sequence)-puromycin resistance gene,
\nthe CAG promoter, a floxed 3x SV40pA stop cassette (orange triangles), a 3x pA (SV40pA-TKpA-SV40pA)
\nstop cassette flanked by FRT sites (blue triangles), effector gene (green box), and WPRE-SV40pA was
\nknocked in at the\u00a0Rosa26<\/em>\u00a0locus. R26-CAG-LF-Effector was modified by the germline excision
\nof theFrt<\/em>-flanked stop cassette using the pCAG-FLPe mouse to produce a Cre-dependent
\neffector line (R26-CAG-LoxP-Effector). The germline excision of the floxed stop cassette using
\nthe TNAP-Cre mouse converts the R26-CAG-LF-Effector line to a Flp-dependent effector line (R26-CAG-FRT-Effector).<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\n

The Cre-loxP<\/em>\u00a0and Flp-FRT systems are very useful tools for time- and space-specific gene knockout [1]. Drs. Imayoshi and Kageyama generated multifunctional fluorescent reporter mice that strongly express monomeric teal fluorescent protein (mTFP1) after Cre- and\/or Flp-mediated recombination [2]. mTFP1 is an improved variant of tetrameric cyan fluorescent protein (CFP) cFP484 from\u00a0Clavularia coral<\/em>. The reporter cassette was inserted into the\u00a0Gt(ROSA)26Sor<\/em>\u00a0locus (R26-CAG-LF-mTFP1). The germline excision of the\u00a0Frt<\/em>-flanked stop cassette in R26-CAG-LFmTFP1 generates a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the\u00a0loxP<\/em>-flanked stop cassette is excised in the germline, requires only Flp to activate mTFP1 expression. These reporter mice are useful for monitoring Cre\/Flp expression in tissues, and tracing the lineage of cells in embryos and adult mice at desired time points.<\/p>\n

Drs. Imayoshi and Kageyama recently reported new mouse strains that express modifiedNatronomonas pharaonis<\/em>\u00a0halorhodopsin (eNpHR2)-EYFP fusion proteins after Cre- and\/or Flp-mediated recombination in order to silence neural activity\u00a0in vivo<\/em>\u00a0[3]. Optogenetic strategies for controlling neuronal activity have been widely employed for mapping functional circuits within the nervous system. These mouse strains also offer powerful tools for light-induced silencing of neural activity in genetically defined cell populations.<\/p>\n<\/div>\n

 <\/p>\n\n\n\n\n\n\n
Fluorescent
\nreporter mouse
\nstrains<\/td>\n		:<\/td>\n\u00a0Cre-&Flp-dependent mTFP1 mouse (R26-CAG-LF-mTFP1): RBRC05146
\nB6;129S6-Gt(ROSA)26Sor<tm1(CAG-mTFP1)Imayo>\/ImayoRbrc<\/a>
\nCre-dependent mTFP1 mouse (R26-CAG-LoxP-mTFP1): RBRC05147
\nB6;129S6(B6)-Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo>\/ImayoRbrc<\/a>
\nFlp-dependent mTFP1 mouse (R26-CAG-FRT-mTFP1): RBRC05148
\nB6;129S6(B6)-Gt(ROSA)26Sor<tm1.2(CAG-mTFP1)Imayo>\/ImayoRbrc<\/a>
\nmTFP1 mouse (R26-CAG-mTFP1): RBRC05149
\nB6;129S6(B6)-Gt(ROSA)26Sor<tm1.3(CAG-mTFP1)Imayo>\/ImayoRbrc<\/a><\/td>\n<\/tr>\n
Knock-in mice
\nconditionally
\nexpressing
\nhalorhodopsin
\neNpHR2.0<\/td>\n		:<\/td>\nR26-CAG-LF- eNpHR2-EYFP mouse: RBRC05150
\nB6;129S6-Gt(ROSA)26Sor<tm1(CAG-eNpHR2\/EYFP)Imayo>\/ImayoRbrc<\/a>
\nR26-CAG-LoxP- eNpHR2-EYFP mouse: RBRC05151
\nB6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-eNpHR2\/EYFP)Imayo>\/ImayoRbrc<\/a>
\nR26-CAG-FRT- eNpHR2-EYFP mouse: RBRC05152
\nB6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-eNpHR2\/EYFP)Imayo>\/ImayoRbrc<\/a><\/td>\n<\/tr>\n
Depositor<\/td>\n:<\/td>\nDrs. Itaru Imayoshi and Ryoichiro Kageyama<\/strong>
\n Institute for Virus Research, Kyoto University<\/strong><\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\nMouse strains generated by Dr. Imayoshi (link to the depositor\u0081fs homepage)
\nhttp:\/\/imayoshi.web.fc2.com\/Itaru_Imayoshi_Ph.D.\/Mouse_strains.html<\/a><\/p>\n\n\n\n\n\n
[1]<\/td>\nImayoshi I, Sakamoto M, Kageyama R. Genetic methods to identify and manipulate newly born neurons in the adult brain.\u00a0Front Neurosci.; 5:64, 2011<\/a>.<\/td>\n<\/tr>\n
[2]<\/td>\nImayoshi I, Hirano K, Sakamoto M, Miyoshi G, Imura T, Kitano S, Miyachi H, Kageyama R. A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination.\u00a0Neurosci Res.; 73(1):85-91, 2012<\/a>.<\/td>\n<\/tr>\n
[3]<\/td>\nImayoshi I, Tabuchi S, Hirano K, Sakamoto M, Kitano S, Miyachi H, Yamanaka A, Kageyama R. Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0.\u00a0Neurosci Res.; 75(1):53-8, 2013<\/a>.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Multifunctional Rosa26 reporter mouse strain B6;129S6-Gt(ROSA)26Sor<tm1(CAG-mTFP1)Imayo>\/ImayoRbrc\u00a0\u00a0RBRC05146 Courtesy of Dr. Itaru Imayoshi, Kyoto University Schematic illustration of the Cre- […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8465","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/8465","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=8465"}],"version-history":[{"count":12,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/8465\/revisions"}],"predecessor-version":[{"id":18095,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/8465\/revisions\/18095"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=8465"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}