{"id":8638,"date":"2013-05-16T13:23:50","date_gmt":"2013-05-16T04:23:50","guid":{"rendered":"http:\/\/mus.brc.riken.jp:8001\/?page_id=8638"},"modified":"2021-04-07T14:14:43","modified_gmt":"2021-04-07T05:14:43","slug":"may_2013_mm","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/mouse_of_month\/may_2013_mm","title":{"rendered":"May 2013 Regulating gene expression in photoreceptor precursor cells<\/em>"},"content":{"rendered":"

\"mouse_of_month_201305\"<\/a><\/p>\n\n\n\n
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Regulating gene expression in photoreceptor precursor cells<\/em><\/strong><\/h2>\n
B6.Cg-Tg(Crx-cre\/ERT2)1Tfur\/TfurRbrc RBRC05559<\/a><\/h5>\n

\"mn1305_0101\"<\/p>\n

Courtesy of Takahisa Furukawa, M.D., Ph.D., Osaka University<\/p>\n

X-gal staining of sections isolated from a\u00a0Crx-cre\/ERT2; CAG-CAT-Z<\/em>\u00a0mouse with tamoxifen. Application of tamoxifen induced Cre-mediated recombination of the LacZ reporter transgene in the developing photoreceptor layer of the\u00a0CAG-CAT-Z<\/em>\u00a0reporter mouse (provided by Dr. J. Miyazaki, Osaka University). ONL: outer nuclear layer, INL: inner nuclear layer, GCL: ganglion cell layer.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Cone-rod homeobox containing gene (CRX) is a transcription factor that is predominantly expressed in developing and mature photoreceptor cells in the retina, and plays a crucial role in the terminal differentiation of rods and cones.\u00a0Crx<\/em>\u00a0is one of the earliest expressed genes in photoreceptor precursor cells [1-4]. To obtain the regulation of\u00a0Cre<\/em>\u00a0expression in photoreceptor precursor cells, Furukawa and colleagues established a transgenic mouse line (Crx-CreERT2<\/em>) that expresses\u00a0CreERT2<\/em>\u00a0under the control of a 2-kb promoter fragment from the\u00a0Crx<\/em>\u00a0gene [5, 6]. Application of tamoxifen to\u00a0Crx-CreERT2; CAG-CAT-Z<\/em>\u00a0indicator mice induced Cre-mediated recombination of a LacZ reporter transgene in the developing photoreceptor layer. They also inactivated the retina and anterior neural fold homeobox (Rax<\/em>) gene in early postmitotic photoreceptor precursor cells using the CreERT2 system and revealed that the expression of the transcription factor orthodenticle homolog 2 (Drosophila) (Otx2<\/em>) was severely attenuated in the\u00a0Rax<\/em>-induced conditional knockout retina [6].\u00a0Crx-CreERT2<\/em>\u00a0transgenic mice allow us to regulate gene expression in photoreceptor precursor cells from the embryonic to the adult stage.\n<\/div>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n
Related strains<\/td>\n:<\/td>\n129.B6C3-Tg(Crx-cre)1Tfur\/TfurRbrc\u00a0\u00a0RBRC05426<\/a>
\n129S6;B6C3-Tg(Crx-EGFP)G3Tfur\/TfurRbrc\u00a0\u00a0RBRC05560<\/a>
\n129(Cg)-Rax<tm1.1Tfur>\/TfurRbrc\u00a0\u00a0RBRC05558<\/a>
\nB6.Cg-Tg(Crx-rtTA)G2Tfur\/TfurRbrc\u00a0\u00a0RBRC05562<\/a><\/td>\n<\/tr>\n
Depositor<\/td>\n:<\/td>\nTakahisa Furukawa, M.D., Ph.D.<\/strong>
\nInstitute for Protein Research, Osaka University<\/strong><\/td>\n<\/tr>\n
References<\/td>\n:<\/td>\n\u00a0[1]<\/td>\nFurukawa T, Morrow EM, Cepko CL. Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation.\u00a0Cell; 91(4):531-41, 1997.<\/a><\/td>\n<\/tr>\n
\u00a0[2]<\/td>\nFurukawa T, Morrow EM, Li T, Davis FC, Cepko CL. Retinopathy and attenuated circadian entrainment in Crx-deficient mice.\u00a0Nat Genet.; 23(4):466-70, 1999.<\/a><\/td>\n<\/tr>\n
\u00a0[3]<\/td>\nNishida A, Furukawa A, Koike C, Tano Y, Aizawa S, Matsuo I, Furukawa T. Otx2 homeobox gene controls retinal photoreceptor cell fate and pineal gland development.\u00a0Nat Neurosci.; 6(12):1255-63, 2003.<\/a><\/td>\n<\/tr>\n
\u00a0[4]<\/td>\nMuranishi Y, Sato S, Inoue T, Ueno S, Koyasu T, Kondo M, Furukawa T. Gene expression analysis of embryonic photoreceptor precursor cells using BAC-Crx-EGFP transgenic mouse.\u00a0Biochem Biophys Res Commun.; 392(3):317-22, 2010.<\/a><\/td>\n<\/tr>\n
\u00a0[5]<\/td>\nFurukawa A, Koike C, Lippincott P, Cepko CL, Furukawa T. The mouse Crx 5\u2019-upstream transgene sequence directs cell-specific and developmentally regulated expression in retinal photoreceptor cells.\u00a0J Neurosci.; 22(5):1640-7, 2002.<\/a><\/td>\n<\/tr>\n
\u00a0[6]<\/td>\nMuranishi Y, Terada K, Inoue T, Katoh K, Tsujii T, Sanuki R, Kurokawa D, Aizawa S, Tamaki Y, Furukawa T. An essential role for RAX homeoprotein and NOTCH-HES signaling in Otx2 expression in embryonic retinal photoreceptor cell fate determination.\u00a0J Neurosci.; 31(46):16792-807, 2011.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Regulating gene expression in photoreceptor precursor cells B6.Cg-Tg(Crx-cre\/ERT2)1Tfur\/TfurRbrc RBRC05559 Courtesy of Takahisa Furukawa, M.D., Ph.D., Osaka University X-gal staining of sections isola […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":198,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8638","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/8638","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=8638"}],"version-history":[{"count":14,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/8638\/revisions"}],"predecessor-version":[{"id":18158,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/8638\/revisions\/18158"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/198"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=8638"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}