{"id":8861,"date":"2013-11-18T15:56:43","date_gmt":"2013-11-18T06:56:43","guid":{"rendered":"http:\/\/mus.brc.riken.jp:8001\/?page_id=8861"},"modified":"2021-01-29T14:13:34","modified_gmt":"2021-01-29T05:13:34","slug":"nov_2013","status":"publish","type":"page","link":"http:\/\/mus.brc.riken.jp\/ja\/current_tech\/nov_2013","title":{"rendered":"BRC Current Technology November 2013"},"content":{"rendered":"\n\n\n\n\n
\n\"RIKEN<\/td>\n
\nBRC Current Technology<\/span>
\nNovember 2013<\/span><\/a><\/td>\n<\/tr>\n
\n

8. \u9855\u5fae\u64cd\u4f5c\u80da\u7531\u6765ES\u7d30\u80de\u306e\u6a39\u7acb<\/h1>\n

\u7cfb\u7d71\u4fdd\u5b58\u304a\u3088\u3073\u907a\u4f1d\u5b66\u7814\u7a76\u306e\u30ea\u30bd\u30fc\u30b9<\/h2>\n

\u30de\u30a6\u30b9\u3067\u306f\u3001\u9855\u5fae\u6388\u7cbe\u3084\u4f53\u7d30\u80de\u6838\u79fb\u690d\u6280\u8853\u306a\u3069\u306b\u3088\u308a\u3001\u5bb9\u6613\u306b\u7279\u6b8a\u306a\u80da\u3092\u4f5c\u51fa\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3042\u308b\u3002\u3053\u306e\u3088\u3046\u306b\u3057\u3066\u4f5c\u51fa\u3057\u305f\u80da\u3092\u80da\u76e4\u80de\u307e\u3067\u767a\u751f\u3055\u305b\u3001\u4e00\u5b9a\u306e\u6761\u4ef6\u4e0b\u3067\u57f9\u990a\u3059\u308b\u3068\u3001\u80da\u6027\u5e79\u7d30\u80de\uff08ES\u7d30\u80de\uff09\u304c\u6a39\u7acb\u3067\u304d\u308b\u3002\u3053\u306e\u6280\u8853\u3092\u5fdc\u7528\u3057\u3066\u3001\u3053\u308c\u307e\u3067\u306b\u69d8\u3005\u306a\u6027\u8cea\u3092\u6301\u3064ES\u7d30\u80de\u304c\u6a39\u7acb\u3055\u308c\u3066\u304d\u305f\u3002\u4f8b\u3048\u3070\u3001\u9855\u5fae\u6388\u7cbe\u6280\u8853\u3092\u7528\u3044\u308b\u3068\u3001\u30e9\u30dc\u30de\u30a6\u30b9\u3068\u91ce\u751f\u7531\u6765\u30de\u30a6\u30b9\u306eF1\u4ea4\u96d1\u80da\u3092\u81ea\u5728\u306b\u4f5c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u5bfe\u7acb\u907a\u4f1d\u5b50\u591a\u578b(allelic polymorphism)\u3092\u6301\u3064ES\u7d30\u80de\u304c\u6a39\u7acb\u3067\u304d\u308b[1](\u4e0a\u56f3A)\u3002\u307e\u305f\u3001\u540c\u69d8\u306b\u9855\u5fae\u6388\u7cbe\uff08\uff12\u500b\u306e\u7cbe\u5b50\u7d30\u80de\u6838\u6ce8\u5165\u3001\u4e0a\u56f3B\uff09\u3068\u9664\u6838\u6280\u8853\u3092\u4f75\u7528\u3059\u308b\u3053\u3068\u3067\u3001\u5bfe\u7acb\u907a\u4f1d\u5b50\u591a\u578b\u3092\u6301\u3064\u96c4\u6027\u767a\u751f\u80da(androgenetic)\u7531\u6765ES\u7d30\u80de\u3082\u6a39\u7acb\u3067\u304d\u308b[2]\u3002\u3053\u308c\u3089\u306eES\u7d30\u80de\u306f\u3001\u30b2\u30ce\u30e0\u5237\u8fbc\u307f\u306a\u3069\u306e\u907a\u4f1d\u5b66\u7814\u7a76\u306b\u7528\u3044\u3089\u308c\u3066\u3044\u308b\u3002\u4e00\u65b9\u3001\u6838\u79fb\u690d\u30af\u30ed\u30fc\u30f3\u80da\u304b\u3089\u306f\u3001\u30c9\u30ca\u30fc\u4f53\u7d30\u80de\u3068\u5168\u304f\u540c\u4e00\u306e\u907a\u4f1d\u7684\u80cc\u666f\u3092\u6301\u3064ES\u7d30\u80de\u3092\u6a39\u7acb\u3067\u304d\u308b\u3053\u3068\u304b\u3089\u3001\u30d0\u30c3\u30af\u30a2\u30c3\u30d7\u7684\u306a\u7cfb\u7d71\u4fdd\u5b58\u6cd5\u306b\u7528\u3044\u3089\u308c\u308b[3]\uff08\u4e0a\u56f3C\u304a\u3088\u3073D\uff09\u3002\u307e\u305f\u3001\u30ea\u30f3\u30d1\u7403\u7531\u6765\u306e\u6838\u79fb\u690dES\u7d30\u80de\u306f\u3001\u30ea\u30f3\u30d1\u7403\u7279\u6709\u306eDNA\u518d\u69cb\u6210\u3092\u6709\u3057\u3066\u3044\u308b\u3053\u3068\u304b\u3089\u3001\u514d\u75ab\u5b66\u306e\u7814\u7a76\u306b\u7528\u3044\u308b\u3053\u3068\u304c\u3067\u304d\u308b [4]\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u9855\u5fae\u6388\u7cbe\u3084\u6838\u79fb\u690d\u6280\u8853\u306f\u3001\u500b\u4f53\u3092\u751f\u307f\u51fa\u3059\u3060\u3051\u3067\u306a\u304f\u3001\u7cfb\u7d71\u4fdd\u5b58\u7528\u3042\u308b\u3044\u306f\u7814\u7a76\u7528\u306e\u69d8\u3005\u306aES\u7d30\u80de\u3092\u4f5c\u51fa\u3059\u308b\u5f37\u529b\u306a\u30c4\u30fc\u30eb\u3067\u3082\u3042\u308b\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n
References:<\/td>\n<\/tr>\n
[1] Shinmen et al. Mol Reprod Dev 74:1081-1088, 2007<\/a><\/td>\n<\/tr>\n
[2] Miki et al. Genesis 47:155-160, 2009<\/a><\/td>\n<\/tr>\n
[3] Wakayama et al. Science. 292:740-743, 2001.<\/a><\/td>\n<\/tr>\n
[4] Inoue et al. Curr Biol 15:1114-1118, 2005.<\/a><\/td>\n<\/tr>\n
\u7406\u7814\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u30bb\u30f3\u30bf\u30fc\uff08\u7d30\u80de\u6750\u6599\u958b\u767a\u5ba4<\/a>\uff09\u304c\u4fdd\u6709\u3059\u308b\u9855\u5fae\u6388\u7cbe\u307e\u305f\u306f\u6838\u79fb\u690d\u80da\u7531\u6765ES\u7d30\u80de<\/td>\n<\/tr>\n
(C57BL\/6 x MSM\/Ms) F1 ES\u7d30\u80de\uff1a\u00a0AES0131<\/a>\u3001AES0132<\/a>\u3001AES0133<\/a><\/td>\n<\/tr>\n(C57BL\/6 x JF1\/Ms) F1\u96c4\u6027\u767a\u751f\u80daES\u7d30\u80de\uff1a AES0147\u3001AES0148\u3001AES0149\uff08\u63d0\u4f9b\u958b\u59cb\u4e88\u5b9a\uff09<\/td>\n<\/tr>\n
\u4f53\u7d30\u80de\u7531\u6765ES\u7d30\u80de\uff1a\u00a0AES0012<\/a>\u00a0\u4ed6\u591a\u6570<\/td>\n<\/tr>\n
T\u7d30\u80de\u7531\u6765ES\u7d30\u80de\uff1aAES0127<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

BRC Current Technology November 2013 8. \u9855\u5fae\u64cd\u4f5c\u80da\u7531\u6765ES\u7d30\u80de\u306e\u6a39\u7acb \u7cfb\u7d71\u4fdd\u5b58\u304a\u3088\u3073\u907a\u4f1d\u5b66\u7814\u7a76\u306e\u30ea\u30bd\u30fc\u30b9 References: [1] Shinmen et al. Mol Reprod Dev 74:1081-1088, 2007 [2] Miki et al. Genesis 47:155-160, 2009 [3] Wakayama et al. S […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":3268,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8861","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/8861","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=8861"}],"version-history":[{"count":9,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/8861\/revisions"}],"predecessor-version":[{"id":17899,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/8861\/revisions\/17899"}],"up":[{"embeddable":true,"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3268"}],"wp:attachment":[{"href":"http:\/\/mus.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=8861"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}