Strain Information | |
---|---|
Image | |
BRC No. | RBRC06536 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Uhrf1<tm2.1Hko> |
Former Common name | NP95 cond ver.2, 6-Uhrf1 floxed |
H-2 Haplotype | |
ES Cell line | M1 [(C57BL/6 x 129)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Haruhiko Koseki, RIKEN Center for Integrative Medical Sciences. M1 ES cell were used to generate the mutant mice. |
Strain description | Uhrf1 gene floxed mice. Gene targeting for the Np95 (also known as Uhrf1, ICBP90) allele was done by flanking exons 4 and 5 with Cre-loxP sequences. Conditional deletion of Np95 (exons 4,5) can be achieved by crossing the mice with specific cre deleters such as CreERT2. The Np95 protein is an epigenetic reader and writer molecule, involved in maintenance DNA methylation (Sharif et al., 2007), recognition of H3K9 di/tri methylation (Rothbart et al., 2012) and ubiquitination of histone H3 (Nishiyama et al., 2013). The constitutive null (Np95-KO) mice are embryonically lethal post 9.5 dpc. Ablation of Np95 causes genomewide loss of DNA methylation marks (Sharif et al., 2007). This conditional allele would be useful to study the roles of Np95 in DNA methylation and regulation of other epigenetic modifications such as H3K9 methylation and H3 ubiquitination, by combining with tissue specific Cre deleters |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Nature, 450, 908-912 (2007). 17994007 Nat Immunol. 15, 571-579 (2014). 24777532 Nature, 502, 249-253 (2013). 24013172 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Uhrf1ubiquitin-like, containing PHD and RING finger domains, 117Uhrf1<tm2.1Hko>targeted mutation 2.1, Haruhiko Koseki Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site17Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP |
Ordering Information | |
---|---|
Donor DNA | phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Uhrf1 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Haruhiko Koseki (RIKEN) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month May 2015 |
BRC mice in Publications |
---|
No Data |