Strain Data Sheet

RBRC10606

Strain Information

Image
BRC No.RBRC10606
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Slc32a1-cre)65Kzy
Former Common nameVGAT-Cre (Tg65), VIAAT-Cre (Tg65), SLC32A1-Cre (Tg65)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Shigeyoshi Itohara, RIKEN Center for Brain Science. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained by crossing to C57BL/6J.
Strain descriptionBAC transgenic mice expressing Cre under the control of Slc32a1 (solute carrier family 32 (GABA vesicular transporter), member 1; Vgat) promoter.
Colony maintenance
ReferencesHum. Mol. Genet., 22(23) 4784-4804 (2013). 23922229

Health Report

Examination Date / Room / Rack2024/02/19Room:4-ARack:H
2023/11/20Room:4-ARack:H
2023/08/21Room:4-ARack:H
2023/05/22Room:4-ARack:H
2023/02/20Room:4-ARack:H
2022/11/21Room:4-ARack:H
2022/08/22Room:4-ARack:H
2022/05/23Room:4-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase2cre

Ordering Information

Donor DNAmouse VGAT genomic DNA, SV40 large T antigen nuclear localization signal (NLS), Phage P1 Cre, SV40 Transcription termination region, E. coli Kanamycin resistance gene, Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-392P11
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 22(23) 4784-4804 (2013).
DepositorShigeyoshi Itohara (RIKEN CBS)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data