Strain Information | |
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Image | |
BRC No. | RBRC11127 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129S2-Eqtn<tm1Ktos> |
Former Common name | Eqtn-KO, Eqtn<-/-> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Kiyotaka Toshimori, Chiba university in 2020. Mice were generated using R1 ES cells derived from 129. Mice were further backcrossed to C57BL/6J for 5 generations. Male homozygous mutants exhibit decreased fertility. |
Strain description | Eqtn knockout mice. Pgk-neo fragment replaced Exon 1 to 5 of the Eqtn gene. |
Colony maintenance | |
References | Reproduction, 156(6):579-590 (2018). 30328350 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP4loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | P1 phage loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter) and poly A signal, E coli Neomycin, mouse Eqtn genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Reproduction, 156(6):579-590 (2018).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Kiyotaka Toshimori (Chiba University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |