Strain Data Sheet

RBRC10901

Strain Information

Image
BRC No.RBRC10901
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-P2rx4<em1Rmur>
Former Common nameP2X4-flox
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masahiro Yamamoto, Osaka University in 2015, and deposited by Rieko Muramatsu, National Center of Neurology and Psychiatry in 2019. This strain was generated with the CRISPR/Cas9 using fertilized eggs derived from C57BL/6J. C57BL/6J genetic background.
Strain descriptionFloxed P2rx4 mouse. A pair of loxP flanks exon 2 of the P2rx4 gene.
Colony maintenance
References
The P2X4 receptor is required for neuroprotection via ischemic preconditioning.
Ozaki T, Muramatsu R, Sasai M, Yamamoto M, Kubota Y, Fujinaka T, Yoshimine T, Yamashita T
Sci. Rep., 6, 25893 (2016). 27173846

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
P2rx4
MGI:1338859		purinergic receptor P2X, ligand-gated ion channel 45P2rx4<em1Rmur> endonuclease-mediated mutation 1, Rieko Muramatsu
loxP phage P1 loxP5loxP
loxP phage P1 loxP5loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAPhage P1 loxP sites, mouse P2rx4 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Scientific Reports (2016) 6, 25893. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorRieko Muramatsu (National Center of Neurology and Psychiatry)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data