Strain Data Sheet

RBRC10931

Strain Information

Image
BRC No.RBRC10931
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(PDGFB-CLSTN1<*>CTF)Tg54Shat
Former Common nameHuman Alcadein alpha/calcyntenin 1 CTF Tg mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped and deposited by Norio Takei, Saori Hata, Toshiharu Suzuki, Hokkaido University in 2019. Transgenic DNA was injected to fetilized eggs derived from BDF1. C57BL/6J congenic strain by backcrossing for 15 generations.
Strain descriptionTransgenic mice expressing a c-terminal fragment of the human Alc alpha under the control of PDGF-beta promoter.
Colony maintenance
ReferencesJ. Biol. Chem., 284, 36024-36033 (2009). 19864413
J. Biol. Chem., 290, 987-995 (2015). 25406318

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CLSTN1calsyntenin 1 (human)UNCLSTN1human PDGF-beta promoter

Ordering Information

Donor DNAhuman PDGF-beta promoter, human Alc alpha cDNA, SV40 polyA sequence
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 284, 36024-36033 (2009). J. Biol. Chem., 290, 987-995 (2015). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Original researcher Drs. Toshiharu Suzuki & Saori Hata is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-proflt research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval form Drs. Toshiharu Suzuki (tsuzuki@pharm.hokudai.ac.jp) and Saori Hata (shata@pharm.hokudai.ac.jp) using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-proit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with Drs. Toshiharu Suzuki & Saori Hata and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact Drs. Toshiharu Suzuki & Saori Hata in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorSaori Hata (Hokkaido University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data