Strain Information | |
---|---|
Image | |
BRC No. | RBRC11036 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | C57BL/6N.Cg-Adam28<em2Osb> |
Former Common name | Adam28<-47616/wt> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Mice were generated using EGR-G01 (ES cell) derived from (129S2 x B6Cr)F1-Tg(CAG/Acr-EGFP). Mice were further crossed to C57BL/6N. C57BL/6N congenic line. |
Strain description | Adam28 knockout mouse generated by the CRISPR/Cas9. 47616 bp deletion in the Adam28 gene. Homozygous mice tend to exhibit decreased fertility. |
Colony maintenance | |
References | Science, 368(6495):1132-1135 (2020). 32499443 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Adam28a disintegrin and metallopeptidase domain 2814Adam28<em2Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
---|---|
Donor DNA | human hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Mouse a part of Adam28 gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 368(6495):1132-1135 (2020).The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
|
BRC mice in Publications |
---|
No Data |