Strain Information | |
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Image | |
BRC No. | RBRC11217 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | STOCK Kif9<em2Osb> Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb |
Former Common name | Kif9<LD/WT> |
H-2 Haplotype | |
ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
Background strain | |
Appearance | |
Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Mice were generated using EGR-G01 (ES cell) derived from (129S2 x B6Cr)F1-Tg(CAG/Acr-EGFP). Mice were further crossed to B6D2F1. Mixed genetic background. |
Strain description | Kif9 knockout mouse generated by the CRISPR/Cas9. 41,902 bp deletion in the Kif9 gene. This stain also has a transgene, Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb. |
Colony maintenance | |
References | FASEB J., 34(4):5389-5400 (2020). doi: 10.1096/fj.201902755R 32072696 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Kif9kinesin family member 99Kif9<em2Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), preproacrsin promoter |
Ordering Information | |
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Donor DNA | human hU6 promoter, CMV CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Mousea part of Kif9 gene, Rabbit beta-globin polyadenylation signal, Mouse proacrosin signal peptide, Mouse acrosin N-ternimal peptide, Jellyfish GFP cDNA, Mouse acrosin promoter |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FASEB J. 2020 Apr;34(4):5389-5400. doi: 10.1096/fj.201902755RRECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |