Strain Data Sheet
RBRC11232
Strain Information | |
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| Image | |
| BRC No. | RBRC11232 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | STOCK Llcfc1<em2Osb> Tg(Clgn-Llcfc1/PA)1Osb |
| Former Common name | Llcfc1<-1024/-1024>, Llcfc1-PA Tg, SOF1 |
| H-2 Haplotype | |
| ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Mixed genetic background. |
| Strain description | Llcfc1 mutant mouse generated by the CRISPR/Cas9. 1024 bp deletion in the Llcfc1 gene. This strain also has a transgene expressing Llcfc1-PA under the control of Clgn promoter. |
| Colony maintenance | |
| References | Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice. Noda T, Lu Y, Fujihara Y, Oura S, Koyano T, Kobayashi S, Matzuk M M, Ikawa M Proc. Natl. Acad. Sci. USA, 117(21):11493-11502 (2020). 32393636 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Llcfc1 MGI:1923856 | LLLL and CFNLAS motif containing 1 | 6 | Llcfc1<em2Osb> MGI:6437831 | endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University | |||
| HBB2 | rabbit beta-globin polyA signal | UN | HBB2 | ||||
| Llcfc1 | LLLL and CFNLAS motif containing 1 | UN | Llcfc1 | mouse Clgn promoter | |||
| PA | PA tag (GVAMPGAEDDVV) (synthetic) human Podoplanin | UN | PA | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Mouse calmegin promoter, Mouse Llcfc1 cDNA, synthetic PA tag, Rabbit beta-globin polyadenylation signal |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 2020 May 26;117(21):11493-11502. doi: 10.1073/pnas.1922650117. Epub 2020 May 11RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |