Strain Data Sheet

RBRC11238

Strain Information

Image
BRC No.RBRC11238
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Cib4<em1Osb>
Former Common nameCib4<wt/-7>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. Homozygous males are infertile.
Strain descriptionCib4 knockout mouse generated by the CRISPR/Cas9. 7 bp deletion in the Cib4 gene.
Colony maintenance
References
CIB4 is essential for the haploid phase of spermatogenesis in micedagger.
Xu Z, Miyata H, Kaneda Y, Castaneda J M, Lu Y, Morohoshi A, Yu Z, Matzuk M M, Ikawa M
Biol. Reprod., 103(2):235-243 (2020). 32430498

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cib4
MGI:1920509		calcium and integrin binding family member 45Cib4<em1Osb>
MGI:6455014		endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAhuman hU6 promoter, CMV CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Mouse a part of Cib4 gene
Research applicationcovid19_progression
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Reprod. 2020 Aug 4;103(2):235-243. doi: 10.1093/biolre/ioaa059RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data