Strain Information | |
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Image | |
BRC No. | RBRC06427 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Gt(ROSA)26Sor<tm1(CAG-SCAT1)Miur> |
Former Common name | Rosa26-CAG-STOP-SCAT1, B6-ROSA26/SCAT1floxed KI |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Ting Liu, Yoshifumi Yamaguchi and Masayuki Miura, Graduate School of Pharmaceutical Sciences, University of Tokyo, and Katsuaki Hoshino and Tsuneyasu Kaisho, RIKEN Research Center for Allergy and Immunology. C57BL/6J and C57BL/6N mixed. |
Strain description | CAG-STOP-CAST1 knock-in mice. A CAG-loxP-neo-loxP-SCAT1-pA cassette was inserted into the Gt(ROSA)26Sor locus. Homozygous mice are viable (fertility is unknown). |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Cell Rep., 8(4):974-982 (2014). 25127135 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-SCAT1)Miur>targeted mutation 1, Masayuki Miura Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ECFPEnhanced Cyan Fluorescent Protein (Aequorea victoria)6ECFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)6Venus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal6 |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer,chicken beta-actin promoter, rabbit beta-globin genomic DNA), SCAT1 (Jellyfish ECFP cDNA, caspase-1 cleavage site, Jellyfish Venus cDNA), herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neomycin resistance gene, phage P1 loxP sites, Bovine polyA, SV40 polyA, yeast FRT (flipase recombination target) site, mouse Gt(ROSA)26Sor genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 8(4):974-982 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Masayuki Miura (The University of Tokyo) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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