The 2012 Nobel Prize in Physiology or Medicine was awarded jointly to Sir John B. Gurdon and Shinya Yamanaka “for the discovery that mature cells can be reprogrammed to become pluripotent”. In 2006, Dr. Yamanaka and his colleagues generated induced pluripotent stem (iPS) cells from embryonic and adult fibroblasts in mice by the transfection of four transcription factors, Oct3/4, Sox2, c-Myc, and Klf4. In 2007, his group reported that a similar approach was applicable for human fibroblasts, and generated human iPS cells.
Dr. Yamanaka has kindly deposited his mice and iPS cells at RIKEN BioResource Center. Mouse iPS cells can be induced from fibroblasts derived from Nanog-GFP transgenic mice (RBRC02290) by introducing the candidate genes for pluripotency. The list of deposited mice strains is shown below. For iPS cells derived from laboratory mice and human, please contact RIKEN BioResource Center Cell Bank.
(2012/10/09)
| BRC No. | Type | Strain Name | Gene(s) | Research Application(s) |
| RBRC05521 | Transgene | B6(Cg)-Tg(ECAT15-1/ECAT15-2-EGFP)1Yam | EGFP (Green Fluorescent Protein),Frt (yeast FRT (flippase recombination target) sites) | |
| RBRC01968 | Targeted Mutation | B6.129S4-Visfatin<tm1Yam> | IRES (internal ribosomal entry site (EMCV)),Pbef1 (pre-B-cell colony-enhancing factor 1),lacZ (beta-galactosidase (E. coli)),neo (neomycin resistance gene (E. coli)) | Developmental Biology Research |
| RBRC03077 | Targeted Mutation Congenic | B6.Cg-ECAT15-1 ECAT15-2 | Dppa2 (developmental pluripotency associated 2),Dppa4 (developmental pluripotency associated 4),frt (yeast FRT (flippase recombination target) sites),loxP (phage P1 loxP) | |
| RBRC05519 | Targeted Mutation | B6;129S4(Cg)-ECAT15-1 ECAT15-2 | Dppa2 (developmental pluripotency associated 2),Dppa4 (developmental pluripotency associated 4),frt (yeast FRT (flippase recombination target) sites),loxP (phage P1 loxP) | |
| RBRC05520 | Targeted Mutation | B6;129S4(Cg)-ECAT15-1 ECAT15-2 | Dppa2 (developmental pluripotency associated 2),Dppa4 (developmental pluripotency associated 4),frt (yeast FRT (flippase recombination target) sites),loxP (phage P1 loxP) | |
| RBRC02326 | Targeted Mutation | B6;129S4-Ecat8<tm1Yam> | IRES (internal ribosomal entry site (EMCV)),Tdrd12 (tudor domain containing 12),lacZ (beta-galactosidase (E. coli)),neo (neomycin resistance gene (E. coli)),poly A (SV40 poly A) | Cell Biology Research,Developmental Biology Research |
| RBRC05478 | Targeted Mutation | B6;129S4-ECAT11 | L1td1 | |
| RBRC05518 | Targeted Mutation | B6;129S4-ECAT15-1 ECAT15-2 | Dppa2 (developmental pluripotency associated 2),Dppa4 (developmental pluripotency associated 4),Hyg (hygromycin phasphotransferase (E. coli)),frt (yeast FRT (flippase recombination target) sites),loxP (phage P1 loxP),neo (neomycin resistance gene (E. coli)) | |
| RBRC01966 | Targeted Mutation | B6;129S4-ERas<tm1Yam> | Eras (ES cell-expressed Ras),IRES (internal ribosomal entry site (EMCV)),lacZ (beta-galactosidase (E. coli)),neo (neomycin resistance gene (E. coli)) | |
| RBRC01965 | Targeted Mutation | B6;129S4-Fbxo15<tm1Yam> | Fbxo15 (F-box protein 15),IRES (internal ribosomal entry site (EMCV)),lacZ (beta-galactosidase (E. coli)),neo (neomycin resistance gene (E. coli)) | Developmental Biology Research |
| RBRC01967 | Targeted Mutation | B6;129S4-Nanog<tm1Yam> | IRES (internal ribosomal entry site (EMCV)),Nanog (Nanog homeobox),lacZ (beta-galactosidase (E. coli)),neo (neomycin resistance gene (E. coli)) | Developmental Biology Research |
| RBRC01969 | Targeted Mutation and Transgene | STOCK Tg(CAG-EGFP)1Yam Fbxo15tm1Yam | Fbxo15 (F-box protein 15),GFP (Green Fluorescent Protein (Jellyfish)),IRES (internal ribosomal entry site (EMCV)),lacZ (beta-galactosidase (E. coli)),neo (neomycin resistance gene (E. coli)) | Developmental Biology Research |
| RBRC02290 | Transgene | STOCK Tg(Nanog-GFP,Puro)1Yam | GFP (Green Fluorescent Protein (Jellyfish)),IRES (internal ribosomal entry site (EMCV)),frt (yeast FRT (flippase recombination target) sites),poly A (bovine growth hormone poly adenylation signal sequence),puro (puromycin (Streptomyces alboniger)) | Developmental Biology Research,Cell Biology Research,Fluorescent Proteins/lacZ System,FLP/frt system |
