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Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

Today’s tool for functional analysis / Today’s model for human disease





DateTitleBRC No.DescriptionReferences
19.04.01Cre-driver mice for myelinating cells of the central nervous system1)RBRC01461This strain is useful for the elucidation of gene function in oligodendrocyte myelination2).1) Genesis. 2000 Feb;26(2):127-9.
2) Nat Commun. 2016 Nov 23;7:13478.
19.04.01Oct3/4 (Pou5f1) reporter miceRBRC007711)
RBRC060372)
These strains are useful for stem cell research because they express EGFP under the control of pluripotency marker Oct3/43) 4) 5).1) Dev Biol. 2003 Jun 1;258(1):209-25.
2) Development. 2008 Mar;135(5):909-18.
3) Cell Res. 2011 Jan;21(1):196-204.
4) Dev Biol. 2010 Oct 1;346(1):90-101.
5) Stem Cells. 2009 Feb;27(2):383-9.
19.04.01Fgf5 spontaneous mutant miceRBRC05894

RBRC05895
This strain shows abnormal hair follicle cycle1). In addition, it contributed to the research about spermatogenic stem cells2).1) Exp Anim. 2011;60(2):161-7.
2) Cell Stem Cell. 2019 Jan 3;24(1):79-92. e6.
19.04.01Fstl4 (Mahya) reporter miceRBRC04952This strain is useful for revolutionary genetic research1)In addition, it was used to monitor specific subtypes of retinal ganglion cells2).1) Dev Genes Evol. 2005 Nov;215(11):564-74.
2) Curr Eye Res. 2016 Apr;41(4):558-68.


DateTitleBRC No.DescriptionReferences
19.05.08Ehlers-Danlos syndorome spondylodysplastic type 3
(OMIM: 612350)
model mice
RBRC06217This is a Slc39a13 (disease gene)
knockout mouse strain,
which shows defect of connective tissue
similar to the human condition1) 2).
1) PLoS Genet. 2017 Aug 30;13(8):e1006950.
2) PLoS One. 2008;3(11):e3642.
19.05.08Myoclonic epilepsy of Lafora
(OMIM: 254780)
model mice
RBRC02715This is a Epm2a (disease gene)
knockout mouse strain,
which shows progressive neurodegeneration
similar to the human condition1) 2) 3).
1) Hum Mol Genet. 2017 Dec 15;26(24):4778-4785.
2) Brain. 2014 Mar;137(Pt 3):806-18.
3) Hum Mol Genet. 2002 May 15;11(11):1251-62.
19.05.08Hirschsprung disease susceptibility to 1
(OMIM: 142623)
model mice
RBRC06249This is a Ret (disease gene)
point mutation knockin mouse strain,
which shows megacolon and deafness
similar to the human condition1) 2) 3).
1) Dev Biol. 2011 Jan 15;349(2):160-8.
2) Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):13051-6.
3) Mol Cell Biol. 2004 Sep;24(18):8026-36.
19.05.08Mowat-Wilson syndrome
(OMIM: 235730)
model mice
RBRC01925This is a Zeb2 (disease gene)
knockout mouse strain,
which shows some abnormal phenotypes
similar to the human condition1) 2) 3).
1) Hum Mol Genet. 2007 Jun 15;16(12):1423-36.
2) Am J Hum Genet. 2003 Feb;72(2):465-70.
3) Genesis. 2002 Feb;32(2):82-4.