GP2 on M Cells for the Mucosal Immune Response
Figures show the exocrine pancreas (x200) in a fasted GP2-/- mouse in which the zymogen granules are not recycled and the GP2 is required for the maintenance of digestive enzymes in the exocrine pancreas, data kindly provided by R. Kawashima, Kitasato University, et. al.
Glycoprotein 2 (GP2)-deficient mice were generated by targeted mutagenesis in the 129/SvJ ES cell line. GP2 is a glycophosphatidylinositol (GPI)-anchored membrane-associated protein that is highly expressed in pancreatic zymogen granules and specialized epithelial M cells of Peyer’s patches. In homozygous Gp2-/- mice, the uptake of type-I-piliated bacteria by M cells was significantly decreased, suggesting that GP2 functions as a transcytotic antigen receptor specific to a subset of commensal and pathogenic enterobacteria. The GP2 knockout mice are useful to study the molecular mechanisms of the mucosal immune response and secretory granules in the pancreas.
||Dr. Shin-ichi Fukuoka, Aoyama Gakuin University
||Hase K, Kawano K, Nochi T, Pontes GS, Fukuda S, Ebisawa M, Kadokura K, Tobe T, Fujimura Y, Kawano S, Yabashi A, Waguri S, Nakato G, Kimura S, Murakami T, Iimura M, Hamura K, Fukuoka S, Lowe AW, Itoh K, Kiyono H, Ohno H. Uptake through glycoprotein 2 of FimH+ bacteria by M cells initiates mucosal immune response. Nature;462(7270):226-30,2009.
||Kobayashi K, Yanagihara K, Ishiguro K, Fukuoka S. GP2/THP gene family of self-binding, GPI-anchored proteins forms a cluster at chromosome 7F1 region in mouse genome.Biochem Biophys Res Commun.;322(2):659-64,2004.