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December 2007 Mouse of the Month |
Nanog-GFP Transgenic Mouse
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| Research Application | : | Stem Cell Research Developmental Biology |
 Generation of iPS cells by using Nanog-GFP Tg mouse (Nature, 448, 313-317 (2007)) |
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| Germline-competent iPS cells from Nanog-GFP transgenic mouse. Phase contrast (left) and fluorescence (right) micrographs are shown. |
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Nanog gene is a good candidate marker for maintaining pluripotent differentiation, because of the loss of pluripotent epiblast in Nanog KO mice, the tendency of Nanog-null ES cells to differenciate spontaneously, and the capability of Nanog-overexpressed ES cells to maintain self-renewal independently of leukaemia inhibitory factor (LIF). Nanog-GFP transgene was generated by insertion of GFP-IRES-puromycin resistance gene (Puror) cassette into the 5′ untranslated region of a bacterial artificial chromosome (BAC) containing the mouse Nanog gene. ES cells with the Nanog-GFP transgene were positive for GFP, but became negative when differentiation was induced. Using this system, pluripotent stem cells competent for germline chimaeras can be induced from Nanog-GFP transgenic mouse fibroblasts by introduction of candidate genes for pluripotency.
The iPS cells will be available soon from RIKEN BRC Cell Bank.
| Depositor | : | Dr. Shinya Yamanaka (Kyoto University) |
| Reference | : | Nature (2007) 448:313-7. |
