Cre driver mice for B cell lineage

RIKEN BRC January 2023
Mouse of the Month
Cre driver mice for B cell lineage

B6-Cd19<tm1.1(icre)Yoba> (RBRC11522)


B cell-specific and efficient Cre-mediated recombination activity in CD19-iCre line.
EYFP expression is demonstrated in CD19+ B220+ B cells and CD19 B220 non-B cells from spleen of Cd19iCre/+R26EYFP mice.


B cells are one of the acquired immune cells that play an important role in humoral immunity against pathogens. B cells are derived from hematopoietic stem/progenitor cells in the bone marrow and differentiate into appropriate forms by interactions with immunoglobulin genes and transcription factors. B cells are also involved in the immune response through antibody production, antigen presentation, and secretion of inflammatory cytokines. B cells have also been reported to function as negative regulators of immunity through anti-inflammatory cytokines such as IL-10 and IL-35.
CD19-iCre knock-in mouse (RBRC11522) is a Cre driver mouse that expresses iCre in B cells. A T2A-iCre cassette is inserted in-frame just before the 3′ terminal stop codon of Cd19. Mating with a Cre-reporter strains have been shown to induce highly efficient Cre/loxP recombination throughout B cell lineage from the early B cell stages. Elucidation of the differentiation process and function of B cells and the related molecular network is important not only in the field of basic immunology but also in the clinical aspect. Therefore, effective use of this CD19-iCre knock-in strain is expected.


Keywords : B cells, Cd19, bone marrow, Cre/loxP system, iCre
Depositor : Yoshihiro Baba, Ph.D.
Kyushu University
Strain name : B6-Cd19<tm1.1(icre)Yoba>
RBRC No. : RBRC11522
Reference : Yasuda T, Saito Y, Ono C, Kawata K, Baba A, Baba Y.
Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells.
Sci Rep. 2021 Mar 9;11(1):5524.


January 2023
Saori Mizuno, Ph.D.
Contact: Experimental Animal Division, RIKEN BioResource Research Center (
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