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January 2023 Mouse of the Month |
Cre driver mice for B cell lineage
B6-Cd19<tm1.1(icre)Yoba> (RBRC11522)
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| B cells are one of the acquired immune cells that play an important role in humoral immunity against pathogens. B cells are derived from hematopoietic stem/progenitor cells in the bone marrow and differentiate into appropriate forms by interactions with immunoglobulin genes and transcription factors. B cells are also involved in the immune response through antibody production, antigen presentation, and secretion of inflammatory cytokines. B cells have also been reported to function as negative regulators of immunity through anti-inflammatory cytokines such as IL-10 and IL-35. CD19-iCre knock-in mouse (RBRC11522) is a Cre driver mouse that expresses iCre in B cells. A T2A-iCre cassette is inserted in-frame just before the 3′ terminal stop codon of Cd19. Mating with a Cre-reporter strains have been shown to induce highly efficient Cre/loxP recombination throughout B cell lineage from the early B cell stages. Elucidation of the differentiation process and function of B cells and the related molecular network is important not only in the field of basic immunology but also in the clinical aspect. Therefore, effective use of this CD19-iCre knock-in strain is expected. |
| Keywords | : | B cells, Cd19, bone marrow, Cre/loxP system, iCre | |
| Depositor | : | Yoshihiro Baba, Ph.D. Kyushu University |
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| Strain name | : | B6-Cd19<tm1.1(icre)Yoba> | |
| RBRC No. | : | RBRC11522 | |
| Reference | : | Yasuda T, Saito Y, Ono C, Kawata K, Baba A, Baba Y. Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells. Sci Rep. 2021 Mar 9;11(1):5524. |
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| January 2023 Saori Mizuno, Ph.D. Contact: Experimental Animal Division, RIKEN BioResource Research Center (animal.brc@riken.jp) All materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content. |
