A novel luminescent Tg strain expressing emerald luciferase

RIKEN BRC January 2024
Mouse of the Month

A novel luminescent Tg strain
expressing emerald luciferase

C57BL/6-Tg(CAG-ELuc)1Nkzom (RBRC11951)

Comparison chart

(A) The emerald luciferase expression of the whole body was detected using in vivo imaging technology. Exposure time: 0.2 sec.
(B) An example of experiment using CAG-ELuc mice: generation of endometriotic mouse model.

The CAG-ELuc mice (RBRC11951) is a transgenic mouse expressing emerald luciferase (ELuc) under the control of the CAG promoter [1]. ELuc is a luciferase cloned from the Brazilian click beetle Pyrearinus termitilluminans luciferase and developed as a bioluminescent imaging probe [2]. ELuc emits green light (λmax = 538 nm) when reacted with D-luciferin, a luminescent substrate. In addition, ELuc has been reported to exhibit brighter signals in mammalian cells compared to conventional Fluc from Photinus pyralis. In vivo and ex vivo imaging analyses of CAG- ELuc mice have shown that ELuc is ubiquitously expressed and has a strong luminescence intensity. The CAG- ELuc mouse line was developed to noninvasively and continuously monitor the early stages of endometriosis lesion formation in endometriosis models. However, CAG- ELuc strain is highly applicable not only to endometriosis models, but also to in vivo imaging systems (IVIS) in a variety of situations. It is expected to be used in a wide range of research areas in the future.


Keywords : bioluminescence, luciferase, in vivo imaging system (IVIS)
Depositors : Fuminori Taniguchi, M.D., Ph.D. and Kazuomi Nakamura, Ph.D. (Tottori University)
Strain name : C57BL/6-Tg(CAG-ELuc)1Nkzom
RBRC No. : RBRC11951
References : [1] Wibisono H, Nakamura K, Taniguchi F, Seno M, Morimoto K, Yoshimura Y, Harada T.
Tracing location by applying Emerald luciferase in an early phase of murine endometriotic lesion formation.
Exp Anim. 2022 May 20;71(2):184-192.
[2] Nakajima Y, Yamazaki T, Nishii S, Noguchi T, Hoshino H, Niwa K, Viviani VR, Ohmiya Y.
Enhanced beetle luciferase for high-resolution bioluminescence imaging.
PLoS One. 2010 Apr 2;5(4):e10011.
Related DNA resource : pCAGGS-Cre (RDB08998)


January 2024
Saori Mizuno, Ph.D.
Contact: Experimental Animal Division, RIKEN BioResource Research Center (animal.brc@riken.jp)
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