Experimental Animal Division

June 2006 Mouse of the Month

 

Cre Transgenic Mouse Series_2
Cre recombinase is a 38 kDa enzyme derived from the bacteriophage P1 which specifically recognizes 34 bp loxP sites. Cre has been shown to effectively mediate the excision of DNA flanked by loxP sites. Cre-loxP system is a very useful tool for time-space-specific knock-out the genes which can not be investigated in adults because of their embryonic lethality in conventional gene-targetting.
P7 seminiferous tubules. Blue, recombined spermatogonia.	Ngn3-Cre Promoter : Neurogenin 3 Application : Developmental Biology Expression : Spermatogenesis (spermatogonia) Pancreatic/enteric endocrine precursors Depositor : Dr. Shosei Yoshida (Kyoto University) References : Dev. Biol. 269, 447 (2004) Development 133, 1495 (2006) BRC No. 01456 ngn3/Cre transgenic mouse
	Olig2-CreER* Promoter : Olig2 Application : Neurobiology Expression : Motoneuron and Oligodendrocyte progenitors Depositor : Dr. Hirohide Takebayashi (National Institute for Physiological Sciences) References : Curr. Biol.12, 1157 (2002) Dev. Biol. 293, 358 (2006) BRC No. 01507 Olig2-CreER knock in mouse
*CreER is tamoxifen-inducible Cre recombinase/Estrogen receptor fusion protein.To See other Cre transgenic mice