Jul 2008 Lck-p19Arf Transgenic Mouse


Lck-p19Arf Transgenic Mouse

A Key Molecule for early T cell development

BRC No. RBRC02371 C57BL/6-Tg(Lck-p19Arf)1
BRC No. RBRC02372 C57BL/6-Tg(Lck-p19Arf)2
Research Application : Immunology and Inflammation Research



  The Ink4a-Arf tumor suppressor locus encodes two proteins, p19Arf and p16Ink4, and influences key physiological processes such as replicative senescence, apoptosis, and stem cell self renewal. The Lck-p19Arf transgenic mice carry the gene encoding p19Arf under the control of the Lck proximal promoter. Two independently generated these transgenic mice showed that the number of total thymocytes and percentage of CD4/CD8 double positive (DP) cells was dramatically reduced, whereas the percentage of γδT cells was increased. The overexpression of p19Arf results in thymocyte cell death and inhibits the CD4/CD8 double negative (DN) to DP transition in a p53-dependent manner. In the lineage-negative CD4/CD8 double negative (DN) population, the percentage of DN4 cells was markedly decreased. These transgenic mice are useful for investigating p19Arf function in early T-cell development.


Depositor : Dr. Masaki Miyazaki (Hiroshima University)
Strain developer : Dr. Hiroaki Honda, Dr. Kazuko Miyazaki (Hiroshima University)
Reference : Immunity. 2008 28, 231-245.

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