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July 2023 Mouse of the Month |
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A fluorescent reporter strain for sexing at the preimplantation embryos B6;129B6-Tg(CAG-Su9/tdTomato/T2A/Puro)1GAitn (RBRC11535)
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| Y-mtRFP mice (RBRC11535) have a transgene (CAG-Su9-tdTomato-T2A-Puror) inserted between exon 1 and 2 of the Ddx3y gene on the Y chromosome in the opposite direction to the Ddx3y gene. Y-mtRFP mice ubiquitously express red fluorescent tdTomato, which is distinguishable from green fluorescent protein (GFP), on the Y chromosome. In addition, the inclusion of a mitochondrial locating signal (Su9) into N-terminal of tdTomato avoids a postnatal lethal phenotype that may be due to the cytotoxic properties of tdTomato. It was reported that almost half of the fertilized eggs collected from Y-mtRFP male mice crossed with wild-type female mice showed red fluorescent signals at the pre-implantation stage. This indicates that XY eggs were recognized as tdTomato-positive embryos at the preimplantation stage. Furthermore, it is also reported that X-GFP/Y-mtRFP by mating Y-mtRFP male mice with X-GFP female mice (RBRC02032), in which GFP is ubiquitously expressed on X chromosomes, allow clearer sexing at the preimplantation embryos. The Y-mtRFP strain is a useful fluorescent reporter tool for a wide range of sex chromosome-related research. |
| Keywords | : | sex chromosome, preimplantation embryo, tdTomato, sexing, mitochondria | |
| Depositor | : | Ayako Isotani, Ph.D. (Nara Institute of Science and Technology) | |
| Strain name | : | B6;129B6-Tg(CAG-Su9/tdTomato/T2A/Puro)1GAitn | |
| RBRC No. | : | RBRC11535 | |
| Reference | : | [1] | Hirata W, Tomoda T, Yuri S, Isotani A. Generation of the Y-chromosome linked red fluorescent protein transgenic mouse model and sexing at the preimplantation stage. Exp Anim. 2022 Feb 9;71(1):82-89. |
| Related mouse resource | : | B6;B6C3-Tg(CAG/Acr-EGFP)CX-FM139Osb (RBRC02032) | |
| July 2023 Saori Mizuno, Ph.D. Contact: Experimental Animal Division, RIKEN BioResource Research Center (animal.brc@riken.jp) All materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content. |
