Analysis of Rho/Rho-kinase singnaling pathway

RIKEN BRC June 2005
Mouse of the Month
Analysis of Rho/Rho-kinase singnaling pathway

Conditional Knockout by Transgenesis with Dominant Negative Form of Rho or Rho-kinase
BRC No.01295 Strain name:CAT-RhoA DN/4-18
BRC No.01296 Strain name:CAT-RhoA DN/4-19
BRC No.01294 Strain name:CAT-Rho-K DN/3-1
BRC No.01298 Strain name:CAT-Rho-K DN/5-18
BRC No.01297 Strain name:DBH-Cre/6-8
mn050728_0102 Expression of RhoA DN or Rho-K DN in the spinal motor neurons increased apoptosis. DBH-Cre mice specifically express Cre recombinase in neurons of developing spinal cord, sympathetic ganglion and dorsal root ganglion.
Research Application: cell morphology, cell adhesion, cell motility, and apoptosis These transgenic mice express a dominant-negative form for RhoA (RhoA DN) or for Rho-kinase (Rho-K DN) by using the Cre-loxP system to suppress the activity of these signaling molecules in developing tissues. The signaling through Rho GTPase is important in the regulation of cellular processes such as cell morphology, cell adhesion, cell motility, and apoptosis. The above figure shows an analysis on in vivo role of the Rho/Rho-kinase signaling pathway during development of motor neurons in the spinal cord by crossing CAT-RhoA DN or CAT-Rho-K DN with DBH-Cre transgenic mice.
DEPOSITOR: Dr. Kazuto Kobayashi
Reference: Kobayashi K, Takahashi M, Matsushita N, Miyazaki J, Koike M, Yaginuma H, Osumi N, Kaibuchi K, Kobayashi K. Survival of developing motor neurons mediated by Rho GTPase signaling pathway through Rho-kinase. J Neurosci. 2004 Apr 7;24 (14):3480-8.

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