ERAI transgenic mouse

RIKEN BRC March 2007
Mouse of the Month


ERAI transgenic mouse
A model for monitoring endoplasmic reticulum stress

BRC No. 01099_ERAI mice
Research Application : Cell Biology Research
BRC No.01099 ERAI mice
Fluorescence activity in internal organs of ERAI transgenic mice injected with PBS (left) or tunicamycin (right)


Endoplasmic reticulum (ER) stress is caused by the accumulation of unfolded proteins in the ER lumen, and is associated with vascular and neurodegenerative diseases. ERAI (ER stress-activated indicator) transgenic mice carry human XBP1 and venus (a variant of green fluorescent protein) fusion gene under control of a CAG promoter. The transcripts from ERAI constructs are spliced under ER stress, the spliced mRNA is translated into an XBP1-venus fusion protein, which can be detected by fluorescence of the venus. This strain is useful to monitor physiological and pathological ER stress in vivo.


Depositor :  Dr. Masayuki Miura
(University of Tokyo)
Reference :  Iwawaki T, Akai R, Kohno K, Miura M
A transgenic mouse model for monitoring endoplasmic reticulum stress.
Nature Medicine. 10(1):98-102. (2004)

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