A: B6.B6D2-Tg(CAG-GFP/LC3)53NmzRbrc (RBRC00806), a donor strain in the C57BL/6JJcl to generate other three congenic lines.
B: C.B6D2-Tg(CAG-GFP/LC3)53NmzRbrc (RBRC01413) in the BALB/cAnN.
C: C3.B6D2-Tg(CAG-GFP/LC3)53NmzRbrc (RBRC01414) in the C3H/HeNCrlj.
D: Live imaging of autophagosome in pancreatic tissues of B6-GFP-LC3 mice (RBRC00806). For imaging technology details, please refer Cao L et al. High Resolution Intravital Imaging of Subcellular Structures of Mouse Abdominal Organs Using a Microstage Device. PLoS ONE 2012.
Autophagy (Greek for “self-eating”) is known to have important roles in various physiological and pathological processes such as maintenance of the amino acid pool during starvation, preimplantation development, prevention of neurodegeneration, anti-aging, tumor suppression, clearance of intracellular microbes, and regulation of innate and adaptive immunity [1,2]. Accordingly, there is a growing need among scientists to be able to detect autophagy in vivo. The GFP-LC3#53 transgenic mouse is one of the most widely used in vivo autophagy monitoring tools and was developed by Dr. Noboru Mizushima . This transgenic mouse enables the accurate detection of autophagosome in diverse biological processes. We have extended its capability to other useful inbred strains such as BALB/cAnN (RBRC00641), C3H/HeNCrlj, and MSM/MsRbrc (RBRC00209) by successive backcrossing. The genetic backgrounds of these strains were examined using simple sequence length polymorphism markers to assess congenic status. A genotyping protocol was also established to distinguish homozygous and hemizygous transgenic mice. These autophagy monitoring mice will be useful for assessing autophagy in various mouse disease models.
Mizushima N, Yamamoto A, Matsui M, Yoshimori T, Ohsumi Y. In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker. Mol Biol Cell 15(3):1101-1111, 2004.