May 2016 Whole-body in vivo monitoring using human IL6-luciferase transgenic mice


Whole-body in vivo monitoring using
human IL6-luciferase transgenic mice

B6.Cg-Tg(IL6-luc)Mym Tyr<c-Brd> (RBRC09487)


Courtesy of Masayuki Yamamoto, M.D., Ph.D.

Robust Luc-luminescence in brain and spinal cord of WIM-6 mouse subjected to experimental autoimmune encephalomyelitis (EAE); in vivo imaging analysis from dorsal view and 3D-computed tomography.


IL-6 is a pleiotropic cytokine that is produced in many types of cells upon inflammatory insult [1]. IL-6 functions as a warning signal in the event of infection and tissue damage, therefore has been considered to be a reliable inflammatory marker. Yamamoto and colleagues generated bacterial artificial chromosome (BAC)-based hIL6-luciferase transgenic mice to evaluate inflammatory status in vivo (i.e., WIM-6 mouse; Whole-body in vivo monitoring using human IL6-luciferase transgenic mice) [2]. In the LPS-induced sepsis model, LPS dose- and time-dependent luciferase induction was detected by whole-body monitoring and in vitro luciferase assay using bone marrow-derived macrophages. In experimental autoimmune encephalomyelitis (EAE), WIM-6 mice exhibited robust induction of luciferase luminescence in brain and spinal cord. WIM-6 system also monitored therapeutic efficacy of anti-inflammatory reagents in atopic dermatitis model. Overall, our results demonstrate that WIM-6 mouse system serves as a useful tool for in vivo monitoring of inflammation in various disease models.


Depositor : Masayuki Yamamoto, M.D., Ph.D.
Department of Medical Biochemistry
Tohoku University Graduate School of Medicine
Strain name : B6.Cg-Tg(IL6-luc)Mym Tyr<c-Brd>
RBRC No. : RBRC09487
References : [1] Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity, and disease.Cold Spring Harb Perspect Biol.,; 6(10):a016295, 2014.
[2] Hayashi M, Takai J, Yu L, Motohashi H, Moriguchi T, Yamamoto M. Whole-Body In Vivo Monitoring of Inflammatory Diseases Exploiting Human Interleukin 6-Luciferase Transgenic Mice.Mol Cell Biol.; 35(20):3590-601, 2015.


May 2016
Contact: Shinya Ayabe, Ph.D.
Experimental Animal Division, RIKEN BioResource Center
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