EGFP-Akt Transgenic Mouse

RIKEN BRC November 2007
Mouse of the Month


EGFP-Akt Transgenic Mouse

A model for visualization of PI(3,4,5)P3 dynamics

BRC No. 01155_EGFP-Akt-2 transgenic mouse
BRC No. 01156_EGFP-Akt-3 transgenic mouse

Research Application : Cancer Research, Cell Biology Research,
General Purpose, Metabolism Research, and
Immunology and Inflammation Research
BRC No.01156 EGFP-Akt-3 transgenic mouse
Concentrated fluorescent activity of AktPH-GFP at the leading edge (Arrow Heads) during chemotactic peptide-stimulated neutrophil migration was visualized by confocal fluorescence microscopy.


  PI3Ks (phosphoinositide 3-kinases) regulate many critical cellular responses by producing PI(3,4,5)P3 (phosphatidylinositol 3,4,5-trisphosphate). The EGFP-Akt transgenic mice express a fluorescent bioprobe for PI(3,4,5)P3/PI(3,4)P2 (phosphatidylinositol 3,4-bisphosphate) which is the pleckstrin homology domain of Akt (AktPH) and EGFP fusion protein. The AktPH-GFP fluorescence shows defused localization in cytoplasm, however the fluorescence is concentrated at the leading edge during fMet-Leu-Phe (N-formylmethionyl-leucylphenylalanine; chemotactic peptide)-stimulated neutrophil migration. These transgenic mice are useful for studying PI(3,4,5)P3 metabolism in physiological and pathological situations by crossing your mutant mice.


Depositor :  Dr. Takehiko Sasaki (Akita University) (Japanese page)
References :  Nature Cell Biol. (2007) 9(1):36-44.
Biochemical Society Transactions (2007) 35(Pt 2):215-8.

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