BRC facility Health Report
Update information
IMPC Search IMPC database

Frequently used strains and publications


Quality Control

Technical information

Customer service


  Order Form


Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

Nov 2007 EGFP-Akt Transgenic Mouse


EGFP-Akt Transgenic Mouse

A model for visualization of PI(3,4,5)P3 dynamics

BRC No. 01155_EGFP-Akt-2 transgenic mouse
BRC No. 01156_EGFP-Akt-3 transgenic mouse
Research Application : Cancer Research, Cell Biology Research,
General Purpose, Metabolism Research, and
Immunology and Inflammation Research
BRC No.01156 EGFP-Akt-3 transgenic mouse
Concentrated fluorescent activity of AktPH-GFP at the leading edge (Arrow Heads) during chemotactic peptide-stimulated neutrophil migration was visualized by confocal fluorescence microscopy.


  PI3Ks (phosphoinositide 3-kinases) regulate many critical cellular responses by producing PI(3,4,5)P3 (phosphatidylinositol 3,4,5-trisphosphate). The EGFP-Akt transgenic mice express a fluorescent bioprobe for PI(3,4,5)P3/PI(3,4)P2 (phosphatidylinositol 3,4-bisphosphate) which is the pleckstrin homology domain of Akt (AktPH) and EGFP fusion protein. The AktPH-GFP fluorescence shows defused localization in cytoplasm, however the fluorescence is concentrated at the leading edge during fMet-Leu-Phe (N-formylmethionyl-leucylphenylalanine; chemotactic peptide)-stimulated neutrophil migration. These transgenic mice are useful for studying PI(3,4,5)P3 metabolism in physiological and pathological situations by crossing your mutant mice.


Depositor :  Dr. Takehiko Sasaki (Akita University) (Japanese page)
References :  Nature Cell Biol. (2007) 9(1):36-44.
Biochemical Society Transactions (2007) 35(Pt 2):215-8.