[Equipment]
・Thermal Cycler (BM, Biometra)
・Submarine electrophoresis Unit (Cosmo Bio, i-MyRun)
・Microchip Electrophoresis System (Shimadzu, MultiNA MCE-202)
・UV transilluminater & photographic devise (Vilber Bio Imaging, Imaging system E-Box CX5)
・Automated DNA isolation system (KURABO, PI-480)
[Reagents]
・TOYOBO KOD FX (Code.No : KFX-101)
・TOYOBO Marker Gene Detection Kit (MGK-101)
・QIAGEN Multiplex PCR Kit (Cat.No.206143)
・TaKaRa LA Taq with GC Buffer (Cat.No.RR02AG)
・TaKaRa Ex Taq (Cat. No. RR001A)
・TaKaRa Ex Premier DNA Polymerase (Cat.No. RR370B)
・Agarose ME (50013 R)
・NuSieve3:1 Agarose (F5180C)
・Wako Gene Ladder 100 (Cat.No. 316-06951)
・Wako 25 bp DNA step ladder (Cat.No. 292-69001)
・TaKaRa 100 bp DNA Ladder (Code No. 3407A)
・NEB 2-Log DNA ladder (Code No. N0550L)
・Thermo Fisher Scientific pUC19 DNA/MspI (HpaII) Marker (Cat.No. SM0221)
・Thermo Fisher Scientific SYBR™ Gold Nucleic Acid Gel Stain (Cat.No. S11494)
・Thermo Fisher Scientific SYBR safe TM DNA Gel Stain (Cat.No. S33112)
