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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

RIKEN BioResource Research Center Mouse E-mail News No. 152

RIKEN BioResource Research Center Mouse E-mail News No. 152

RIKEN BioResource Research Center (BRC) is pleased to update you with the 
latest information.

======= Contents =======================================================
- Special topic: Mouse of the Month
  "A key transcription factor for innate immunity: IRF3"
- BRC Current Technology
  "Development of reproductive engineering techniques for wild-derived Spretus mice"
  "Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells"
  "In vitro fertilization using first-wave sperm from prepubertal males"
- Today's model for human disease
- Today's tool for functional analysis
- Dr. Yuichi Obata, Senior Adviser, received the IMPC Award of Excellence for 2020
- Bioresources for COVID-19 research in RIKEN BRC
- Mouse genomic variation database has been released
- User publications
- Newly deposited mouse strains

| Special topic: Mouse of the Month

A key transcription factor for innate immunity: IRF3
  RBRC10094  B6.Cg-Irf3<tm2.2Ttg>
  RBRC10095  B6.Cg-Irf3<tm2.1Ttg>

Depositor: Tadatsugu Taniguchi, Ph.D. & Hideyuki Yanai, Ph.D.
           University of Tokyo

Mouse of the Month archive

| BRC Current Technology

Development of reproductive engineering techniques for wild-derived Spretus mice

Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells
In vitro fertilization using first-wave sperm from prepubertal males

BRC Current Technology archive

| Today's model for human disease

Human renin·human angiotensinogen double Tg mice
RBRC01122 hRN 8-12
RBRC01123 hAG 2-5
RBRC02432 (hRN8-12 x hAG2-5)F1

Prolin isomerase Pin1-KO mice
RBRC10368 B6.129P2-Pin1<tm1Tuc>

p53-target gene Tigar-KO mice
RBRC10981 B6.129-Tigar<tm1.2Smat>

Endogenous membrane protein Aqp7-KO mice
RBRC06294 B6;129-Aqp7<tm1Tfun>

Today's model for human disease

| Today's tool for functional analysis

Pro-inflammatory cytokine IL-6 KO mice
RBRC04918 B6;129S2-Il6<tm1Kopf>
RBRC04919 C;129S2-Il6<tm1Kopf>
RBRC05131 D1;129S2-Il6<tm1Kopf>

IL-6-related inflammatory status in vivo imaging mice
RBRC09487 C57BL/6N-Tyr<c-Brd>-Tg(IL6-Luc)

Human IL-6 expression conditionally controllable mice
RBRC09734 C57BL/6N-Tg(CAG-EGFP,-IL6/mCherry)29Kenw
RBRC09735 C57BL/6N-Tg(CAG-EGFP,-IL6/mCherry)56Kenw

Cre reporter mice showing green-to-red fluorescent conversion
RBRC04874 C57BL/6N-Gt(ROSA)26Sor<tm1(CAG-EGFP/tDsRed)Utr>/Rbrc

Cre reporter mice expressing EGFP signal
RBRC09806 B6.Cg-Tg(CAG-cat,-EGFP)39Miya

Cre reporter mice expressing LacZ signal
RBRC02657 B6.129P2-Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito>

Today's tool for functional analysis

| Dr. Yuichi Obata, Senior Adviser, received the IMPC Award of Excellence 2020

 At November 3rd 2020, Dr. Yuichi Obata, Senior Adviser of RIKEN BioResource Center, 
received The International Mouse Phenotyping Consortium (IMPC) Award of Excellence 
for 2020 for the instrumental in the RIKEN BRC joining the IMPC and importantly bringing 
the IMPC to Asia and fostering IMPC activities both in Asia and further afield. 
The award was announced in the Annual Fall Meeting 2020 of the Knockout Mouse 
Phenotyping Project (KOMP2) & IMPC, which was held in Bethesda, USA (online).

| Bioresources for COVID-19 research in RIKEN BRC

 Lists of mouse strains, cell lines and DNA clones useful for COVID-19 R&D 
from RIKEN BRC are now available.

| Mouse genomic variation database has been released

 RIKEN BioResource Research Center have released a mouse genome polymorphism database, 
MoG+, mouse genome database with high added value, to promote advanced use of 
experimental mice. MoG+ provides visualized mouse genomic variation among inbred mouse 
strains established in Japan as well as major classical inbred strains.


| User publications

 The following result has been newly reported by users. We are
honored to introduce new publications to the science community.
Therefore, we look forward to receiving your publications too.

RBRC01828 B6.Cg-Tg(CAG-Cre)CZ-MO2Osb
Harada N, Minami Y, Hanada K, Hanaoka R, Kobayashi Y, Izawa T, Sato T, 
Kato S, Inui H, Yamaji R.
Relationship between gut environment, feces-to-food ratio, 
and androgen deficiency-induced metabolic disorders.
Gut Microbes. 2020 Nov 9;12(1):1817719. doi: 10.1080/19490976.2020.1817719.

RBRC05658 B6.Cg-Siglech<tm1.1Ksat>
Konishi H, Okamoto T, Hara Y, Komine O, Tamada H, Maeda M, Osako F, Kobayashi M, 
Nishiyama A, Kataoka Y, Takai T, Udagawa N, Jung S, Ozato K, Tamura T, Tsuda M, 
Yamanaka K, Ogi T, Sato K, Kiyama H.
Astrocytic phagocytosis is a compensatory mechanism for microglial dysfunction.
EMBO J. 2020 Sep 22;e104464. doi: 10.15252/embj.2020104464.

Morrice N, van Aalten L, McNeilly A, McCrimmon RJ, Pearson ER, Langston R, Sutherland C.
Reducing Glut2 throughout the body does not result in cognitive behaviour differences 
in aged male mice.
BMC Res Notes. 2020 Sep 16;13(1):438. doi: 10.1186/s13104-020-05276-y.

RBRC01939 B6.129X1-Trpv4<tm1Msz>
Weber J, Rajan S, Schremmer C, Chao YK, Krasteva-Christ G, Kannler M, Yildirim AÖ, 
Brosien M, Schredelseker J, Weissmann N, Grimm C, Gudermann T, Dietrich A.
TRPV4 channels are essential for alveolar epithelial barrier function as protection 
from lung edema.
JCI Insight. 2020 Oct 15;5(20):134464. doi: 10.1172/jci.insight.134464.

RBRC01346 C57BL/6-Prkdc<scid>/Rbrc
Nagasaki J, Togashi Y, Sugawara T, Itami M, Yamauchi N, Yuda J, Sugano M, 
Ohara Y, Minami Y, Nakamae H, Hino M, Takeuchi M, Nishikawa H.
The critical role of CD4+ T cells in PD-1 blockade against MHC-II-expressing tumors 
such as classic Hodgkin lymphoma.
Blood Adv. 2020 Sep 8;4(17):4069-4082. doi: 10.1182/bloodadvances.2020002098.

RBRC00806 GFP-LC3#53
Miyake T, Sakai N, Tamai A, Sato K, Kamikawa Y, Miyagawa T, Ogura H, Yamamura Y, 
Oshima M, Nakagawa S, Sagara A, Shinozaki Y, Toyama T, Kitajima S, Hara A, 
Iwata Y, Shimizu M, Furuichi K, Kaneko S, Wada T.
Trehalose ameliorates peritoneal fibrosis by promoting Snail degradation 
and inhibiting mesothelial-to-mesenchymal transition in mesothelial cells.
Sci Rep. 2020 Aug 31;10(1):14292. doi: 10.1038/s41598-020-71230-4.

RBRC09525 C57BL/6J-Ins1<em1(cre)Utr>
Mostafa D, Yanagiya A, Georgiadou E, Wu Y, Stylianides T, Rutter GA, 
Suzuki T, Yamamoto T.
Loss of Β-cell identity and diabetic phenotype in mice caused 
by disruption of CNOT3-dependent mRNA deadenylation.
Commun Biol. 2020 Aug 28;3(1):476. doi: 10.1038/s42003-020-01201-y.

RBRC02095 B6.B6D2-Tg (Th-EGFP)21-31Koba
Kosaka T, Pignatelli A, Kosaka K.
Heterogeneity of tyrosine hydroxylase expressing neurons 
in the main olfactory bulb of the mouse.
Neurosci Res. 2020 Aug;157:15-33. doi: 10.1016/j.neures.2019.10.004.

RBRC05384 B6.Cg-Tg(Prdm14-Venus)1Sait/SaitRbrc
Grosswendt S, Kretzmer H, Smith ZD, Kumar AS, Hetzel S, Wittler L, 
Klages S, Timmermann B, Mukherji S, Meissner A.
Epigenetic regulator function through mouse gastrulation.
Nature. 2020 Aug;584(7819):102-108. doi: 10.1038/s41586-020-2552-x.

RBRC01345 B6.129P2-Emx1<tm1.1(cre)Ito>/ItoRbrc
Mizusawa A, Watanabe A, Yamada M, Kamei R, Shimomura Y, Kitaura Y.
BDK Deficiency in Cerebral Cortex Neurons Causes Neurological Abnormalities 
and Affects Endurance Capacity.
Nutrients. 2020 Jul 29;12(8):2267. doi: 10.3390/nu12082267.

RBRC04895 B6(Cg)-Timd4<tm1Osa>
Min C, Park J, Kim G, Moon H, Lee SA, Kim D, Moon B, Yang S, Lee J, 
Kim K, Cho H, Park J, Lee DH, Lee G, Park D.
Tim-4 functions as a scavenger receptor for phagocytosis of exogenous particles.
Cell Death Dis. 2020 Jul 23;11(7):561. doi: 10.1038/s41419-020-02773-7.

RBRC01059 NC
Chen MC, Chen CS, Wu YW, Yang YY.
Poly-γ-Glutamate microneedles as transdermal immunomodulators for 
ameliorating atopic dermatitis-like skin lesions in Nc/Nga mice.
Acta Biomater. 2020 Sep 15;114:183-192. doi: 10.1016/j.actbio.2020.07.029.

RBRC05154 B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>
Inagaki S, Iwata R, Iwamoto M, Imai T.
Widespread Inhibition, Antagonism, and Synergy 
in Mouse Olfactory Sensory Neurons In Vivo.
Cell Rep. 2020 Jun 30;31(13):107814. doi: 10.1016/j.celrep.2020.107814.

RBRC00209 MSM/Ms
RBRC03730 B6;129S4-Dnmt3a<tm1Enl>
Yagi M, Kabata M, Tanaka A, Ukai T, Ohta S, Nakabayashi K, Shimizu M, 
Hata K, Meissner A, Yamamoto T, Yamada Y.
Identification of distinct loci for de novo DNA methylation 
by DNMT3A and DNMT3B during mammalian development.
Nat Commun. 2020 Jun 24;11(1):3199. doi: 10.1038/s41467-020-16989-w.

RBRC05663 B6.Cg-Bcl6<tm1.1Mtto>
Chikada H, Ida K, Nishikawa Y, Inagaki Y, Kamiya A.
Liver-specific knockout of Β cell lymphoma 6 suppresses progression 
of non-alcoholic steatohepatitis in mice.
Sci Rep. 2020 Jun 16;10(1):9704. doi: 10.1038/s41598-020-66539-z.

RBRC02975 B6.129S-Atg5<tm1Myok>
Shen H, Zhu H, Panja D, Gu Q, Li Z.
Autophagy controls the induction and developmental decline of NMDAR-LTD 
through endocytic recycling.
Nat Commun. 2020 Jun 12;11(1):2979. doi: 10.1038/s41467-020-16794-5.

RBRC02192 B6.129-Mapk14<tm1.2Otsu>
Schnöder L, Gasparoni G, Nordström K, Schottek A, Tomic I, 
Christmann A, Schäfer KH, Menger MD, Walter J, Fassbender K, Liu Y.
Neuronal deficiency of p38 α-MAPK ameliorates symptoms 
and pathology of APP or Tau-transgenic Alzheimer's mouse models.
FASEB J. 2020 Jul;34(7):9628-9649. doi: 10.1096/fj.201902731RR.

RBRC00445 C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb
Sanjo H, Yao T, Katagiri K, Sato T, Matsumura T, Komeya M, Yamanaka H, 
Yao M, Matsuhisa A, Asayama Y, Ikeda K, Kano K, Aoki J, Arita M, Ogawa T.
Antioxidant vitamins and lysophospholipids are critical for 
inducing mouse spermatogenesis under organ culture conditions.
FASEB J. 2020 Jul;34(7):9480-9497. doi: 10.1096/fj.202000245R.

RBRC04395 R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Pirgova G, Chauveau A, MacLean AJ, Cyster JG, Arnon TI.
Marginal zone SIGN-R1+ macrophages are essential for the maturation of 
germinal center Β cells in the spleen.
Proc Natl Acad Sci U S A. 2020 Jun 2;117(22):12295-12305. doi: 10.1073/pnas.1921673117.

RBRC02302 B6.129P2-Lyzs<tm1(cre)Ifo>
Sato H, Taketomi Y, Miki Y, Murase R, Yamamoto K, Murakami M.
Secreted Phospholipase PLA2G2D Contributes to Metabolic Health 
by Mobilizing ω3 Polyunsaturated Fatty Acids in WAT.
Cell Rep. 2020 May 5;31(5):107579. doi: 10.1016/j.celrep.2020.107579.

RBRC02388 B6.129P2-Ly96<tm1Kmiy>
Wang Y, Luo W, Han J, Khan ZA, Fang Q, Jin Y, Chen X, Zhang Y, 
Wang M, Qian J, Huang W, Lum H, Wu G, Liang G.
MD2 activation by direct AGE interaction drives inflammatory diabetic cardiomyopathy.
Nat Commun. 2020 May 1;11(1):2148. doi: 10.1038/s41467-020-15978-3.

RBRC04109 B6.129S6-Calca<tm1Hku>
Jiang Y, Yuan Y, Xiong Y, Wang B, Guo Y, Gong P, Zhang L.
Low-intensity pulsed ultrasound improves osseointegration of 
dental implant in mice by inducing local neuronal production of αCGRP.
Arch Oral Biol. 2020 Jul;115:104736. doi: 10.1016/j.archoralbio.2020.104736.

RBRC06344 C57BL/6-App<tm3(NL-G-F)Tcs>
Susaki EA, Shimizu C, Kuno A, Tainaka K, Li X, Nishi K, Morishima K, 
Ono H, Ode KL, Saeki Y, Miyamichi K, Isa K, Yokoyama C, Kitaura H, 
Ikemura M, Ushiku T, Shimizu Y, Saito T, Saido TC, Fukayama M, Onoe H, 
Touhara K, Isa T, Kakita A, Shibayama M, Ueda HR.
Versatile whole-organ/body staining and imaging based on electrolyte-gel 
properties of biological tissues.
Nat Commun. 2020 Apr 27;11(1):1982. doi: 10.1038/s41467-020-15906-5.

RBRC05494 B6;129P2-Tnfaip3<tm1Homy>
Montarolo F, Perga S, Tessarolo C, Spadaro M, Martire S, Bertolotto A.
TNFAIP3 Deficiency Affects Monocytes, Monocytes-Derived Cells and Microglia in Mice.
Int J Mol Sci. 2020 Apr 18;21(8):2830. doi: 10.3390/ijms21082830.

RBRC02705 B6.Cg-Tg(FucciS/G2/M)#492Bsi
Weitzmann A, Naumann R, Dudeck A, Zerjatke T, Gerbaulet A, Roers A.
Mast Cells Occupy Stable Clonal Territories in Adult Steady-State Skin.
J Invest Dermatol. 2020 Apr 18;S0022-202X(20)31380-4. doi: 10.1016/j.jid.2020.03.963.

RBRC00858 B6;129S6-Bcl2l12/Irf3<tm1Ttg>/TtgRbrc
RBRC01420 B6;129P2-Irf7<tm1Ttg>/TtgRbrc
Sin WX, Yeong JP, Lim TJF, Su IH, Connolly JE, Chin KC.
IRF-7 Mediates Type I IFN Responses in Endotoxin-Challenged Mice.
Front Immunol. 2020 Apr 16;11:640. doi: 10.3389/fimmu.2020.00640.

RBRC01544 B6.129P2-Sirpa<tm1Nog>/Rbrc
Cham LB, Torrez Dulgeroff LB, Tal MC, Adomati T, Li F, Bhat H, Huang A, 
Lang PA, Moreno ME, Rivera JM, Galkina SA, Kosikova G, Stoddart CA, 
McCune JM, Myers LM, Weissman IL, Lang KS, Hasenkrug KJ.
Immunotherapeutic Blockade of CD47 Inhibitory Signaling Enhances Innate and 
Adaptive Immune Responses to Viral Infection.
Cell Rep. 2020 Apr 14;31(2):107494. doi: 10.1016/j.celrep.2020.03.058.

RBRC00886 B6;B6C3-Tg(Acro3-EGFP)01Osb
Fukunaga H, Kaminaga K, Sato T, Watanabe R, Ogawa T, Yokoya A, Prise KM.
The Tissue-Sparing Effect of Spatially Fractionated X-rays for Maintaining 
Spermatogenesis: A Radiobiological Approach for the Preservation of 
Male Fertility after Radiotherapy.
J Clin Med. 2020 Apr 11;9(4):1089. doi: 10.3390/jcm9041089.

RBRC00144 C57BL/6-Ly5.1
Kuroishi T, Bando K, Bakti RK, Ouchi G, Tanaka Y, Sugawara S.
Migratory dendritic cells in skin-draining lymph nodes have 
nickel-binding capabilities.
Sci Rep. 2020 Mar 19;10(1):5050. doi: 10.1038/s41598-020-61875-6.

RBRC06355 B6.Cg-Tg(Nes-EGFP)1Yamm
Sawada R, Nakano-Doi A, Matsuyama T, Nakagomi N, Nakagomi T.
CD44 expression in stem cells and niche microglia/macrophages 
following ischemic stroke.
Stem Cell Investig. 2020 Mar 10;7:4. doi: 10.21037/sci.2020.02.02.

RBRC05564 B6.Cg-Tg(Chx10-cre)G5Tfur
Ikegami K, Shigeyoshi Y, Masubuchi S.
Circadian Regulation of IOP Rhythm by Dual Pathways of Glucocorticoids 
and the Sympathetic Nervous System.
Invest. Ophthalmol. Vis. Sci. 2020 Mar 9;61(3):26. doi: 10.1167/iovs.61.3.26.

RBRC06047 B6.Cg-Hes1<tm1Imayo>
Ma Z, Xu J, Wu L, Wang J, Lin Q, Chowdhury FA, Md Mazumder HH, 
Hu G, Li X, Du W.
Hes1 deficiency causes hematopoietic stem cell exhaustion.
Stem Cells. 2020 Mar 4. doi: 10.1002/stem.3169.

RBRC01834 C57BL/6-Tg(CAG-flpe)36Ito/ItoRbrc
Wada Y, Kikuchi A, Kaga A, Shimizu N, Ito J, Onuma R, Fujishima F, 
Totsune E, Sato R, Niihori T, Shirota M, Funayama R, Sato K, 
Nakazawa T, Nakayama K, Aoki Y, Aiba S, Nakagawa K, Kure S.
Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice.
PLoS Genet. 2020 Feb 26;16(2):e1008628. doi: 10.1371/journal.pgen.1008628.

RBRC02706 B6.Cg-Tg(FucciS/G2/M)#504Bsi
Han L, Choudhury S, Mich-Basso JD, Ammanamanchi N, Ganapathy B, 
Suresh S, Khaladkar M, Singh J, Maehr R, Zuppo DA, Kim J, 
Eberwine JH, Wyman SK, Wu YL, Kühn B.
Lamin B2 Levels Regulate Polyploidization of Cardiomyocyte Nuclei 
and Myocardial Regeneration.
Dev Cell. 2020 Feb 26. doi: 10.1016/j.devcel.2020.01.030.

| Newly deposited mouse strains

Dr. Tsuneyasu Kaisho, RIKEN
RBRC10663 B6.Cg-Xcl1<tm1.2Ksho>
RBRC10664 C.Cg-Xcr1<tm4(cre)Ksho>
RBRC10665 C.Cg-Xcr1<tm1Ksho>
RBRC11161 C57BL/6N-Arg1<tm1.1(KikGR)Ksho>
RBRC11162 C57BL/6N-Xcr1<tm5(XCR1)Ksho>
RBRC11163 B6.Cg-Spib<tm1Ksho>
RBRC11164 C.Cg-Spib<tm1Ksho>

Dr. Yoshio Fujitani, Gunma University
RBRC11097 129;B6-Pias4<em1Yfj>

Dr. Daisuke Kitamura, Tokyo University of Science
RBRC11138 B6.Cg-Blnk<tm1Dkit> Ptprc<a>
RBRC11139 B6.Cg-Dnase1l3<tm1Dkit>
RBRC11140 B6.Cg-Dnase1l3<tm1Dkit> Dffb<tm1Osa>

Dr. Fumihiko Horio, Nagoya University
RBRC11120 A/J-12<SM> Iah1<em1Kobam>

Dr. Takeshi Sakurai, University of Tsukuba
RBRC11137 B6J.B6N-Qrfp<tm2.1(icre)Stak>

Dr. William Weiss, The University of California, San Francisco
RBRC10904 129.Cg-Tg(Th-MYCN)41WawKkad

Dr. Masahito Ikawa, Osaka University
RBRC11018 B6D2-Hdgfl1<em1Osb>
RBRC11025 B6D2-Tmem97<em1Osb>
RBRC11026 B6D2-Ros1<em1Osb>
RBRC11028 B6D2-Spaca6<em2Osb>
RBRC11029 B6D2-Pithd1<em1Osb>
RBRC11035 B6D2-Fam229b<em1Osb>
RBRC11036 C57BL/6N.Cg-Adam28<em2Osb>
RBRC11038 B6D2-Nell2<em1Osb>
RBRC11044 B6D2-Ovch2<em2Osb>
RBRC11046 B6D2-Tmem95<em1Osb>
RBRC11050 B6D2-4921507P07Rik<em1Osb>
RBRC11051 B6D2-Prss51<em1Osb>
RBRC11052 B6D2-Prss55<em1Osb>
RBRC11053 B6D2-Del(9Pate1-Pate3)1Osb
RBRC11054 B6D2-Allc<em1Osb>
RBRC11063 B6D2-Fscb<em1Osb>

Dr. Mamoru Fukuchi, Takasaki University of Health and Welfare
RBRC11093 C57BL/6-Tg(RP24-310A6/Luc)2MftymMfuku

Dr. Tadashi Matsushita, Nagoya University
RBRC11096 B6.129-Myh9<tm1(R702C)Dash>

Dr. Shingo Komura, Gifu University
RBRC11098 B6.Cg-Scx<tm1.1(EGFP)Skom>

Dr. Kiyotaka Toshimori, Chiba University
RBRC11127 B6.129S2-Eqtn<tm1Ktos>
RBRC11128 B6;Cg-Tg(Eqtn-EGFP)1Ktos

Dr. Atsu Aiba, The University of Tokyo
RBRC11066 C57BL/6N-Del(16Pi4ka-Hira)Atai

Dr. Hideyo Ohuchi, Okayama University
RBRC11067 B6;B6CB-Fgf10<tm1Ska>

Dr. Takeshi Takarada, Okayama University
RBRC11090 C57BL6/J-Actb<em1(tetO-PA-cre)ttaka>

Dr. Akio Kihara, Hokkaido University
RBRC11099 B6.129-Elovl1<tm1Akih>
RBRC11100 B6.129-Elovl1<tm1.1Akih>
RBRC11101 C57BL/6J-Slc27a4<em1Akih>
RBRC11102 B6.Cg-Nipal4<tm1Akih>

Dr. Yoichiro Iwakura, Tokyo University of Science
RBRC11103 B6-Il17b<em1Yiw>
RBRC11104 B6-Il17d<em1Yiw>
RBRC11105 B6-C1qtnf3<tm1Yiw>
RBRC11106 C.B6-C1qtnf3<tm1Yiw>
RBRC11112 C.129P2-Cxcr4<tm1Yiw>
RBRC11113 D1.129P2-Cxcr4<tm1Yiw>

Dr. Yasuhisa Matsui, Tohoku University
RBRC11114 B6;129P2-Larp7<tm1Ymat>
RBRC11115 B6;129P2-Rest<tm1Ymat>
RBRC11116 B6.129P2-Tg(Ddx4-RFP)#15Ymat

Dr. Tasuku Tsukamoto, Shionogi & Co., Ltd.
RBRC10951 C57BL/6J-Tg(Col1a1-EGFP)Shi

Dr. Kazuaki Tanaka, Azabu University
RBRC10973 B6.EL-(D2Mit156-D2Mit266)/Azab
RBRC10974 B6.EL-(D2Mit365-D2Mit280)/Azab
RBRC10975 B6.EL-(D9Mit89-D9Mit38)/Azab

Dr. Tohru Yamamoto, Kagawa University
RBRC10977 B6JJcl.Cg-Mdga1<tm1.1Tymo>
RBRC10978 B6NJcl.Cg-Mdga1<tm1.1Tymo>
RBRC10979 B6JJcl.Cg-Mdga2<tm1Tymo>
RBRC10980 B6NJcl.Cg-Mdga2<tm1Tymo>

Dr. Makoto Takano, Kurume University
RBRC10983 B6.Cg-Hcn4<tm1(tTA)Nnka> Hcn4<tm1.1(Luc/EGFP)Nna>

Dr. Takayuki Sakurai, Shinshu University
RBRC10984 B6;B6D2-Tg(CAG-hCas9)2Saku

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