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Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

JoVE 3155 11/18/2011

Mar 2009 FUCCI mice for visualizing cell-cycle in vivo


mouse_of_month_200903

FUCCI mice for visualizing cell-cycle in vivo

Amalgaam MTA ( Japanese · English )
B6.Cg-Tg(Fucci)504Bsi    RBRC 02706
B6.Cg-Tg(Fucci)596Bsi    RBRC 02707

mn0903_0101

 

  Two transgenic strains of fluorescent, ubiquitination-based cell cycle indicator, “FUCCI”, B6.Cg-Tg(Fucci)504Bsi (RBRC02706) and B6.Cg-Tg(Fucci)596Bsi (RBRC02707) mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. The double transgenic mice in which every cell nucleus exhibits either green or red fluorescence will serve as a powerful tool for visualizing the spatio-temporal dynamics of cell-cycle progression in every biological event [1].

 

Developer : Dr. Asako Sakaue-Sawano & Dr. Atsushi Miyawaki (RIKEN)
http://cfds.brain.riken.jp/Fucci.html
Depositor : Amalgaam(MBL)
https://ruo.mbl.co.jp/product/flprotein/fucci.html
Reference : Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Osawa, H., Kashiwagi, S., Fukami, K., Miyata, T., Miyoshi, H., Imamura, T., Ogawa, M., Masai, H. and Miyawaki, A. 2008. Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132: 487-498.