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Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

JoVE 3155 11/18/2011

Ordering Information


1. Search for mouse strain

Search for mouse strain

If you have any questions, please contact our customer service.

E-mail: animal@brc.riken.jp
Fax: +81-29-836-9010

Health Report

Frozen embryos

Frozen sperm

Genotyping PCR Protocol

2. Required documentation

Approval form only when applicable.
(ex. The RECIPIENT of BIOLOGICAL RESOURCE
shall obtain a prior written consent on
use of it from the DEPOSITOR.)

MTA

Order form 
Please select your choice of payment.

Please provide the name and e-mail address of a
person in charge of payment.

Import permit/license
Please obtain an import permit/license prior to
shipping, if required.

3. Payment

We will send an invoice by e-mail as a PDF
attachment.

4. Shipping

We will contact you when the mice are ready.


Order

To request mice strains or frozen materials from the RIKEN BRC, please contact a customer service representative by e-mail ( animal@brc.riken.jp ) or by fax (+81-29-836-9010).

  1. Supply status
  2. Fee
  3. How to order
  4. Terms of use
  5. Payment
  6. Request for publication

1. Supply status

Mouse strains are distributed as living mice. Frozen embryos and sperm are also available on request. The RIKEN BRC customarily supplies two or three breeding pairs.
The center can accommodate requests for other combinations of up to five mice per request.
Other quantities may be negotiated, depending on colony size and current demand at the time of request confirmation.

Supply status Status Icon* Availability level Expected delivery
Live mouse Limited quantities of breeder mice (up to 3 males and 3 females or 6 mice) are available from a production colony.
Two pairs or more are recommended to secure your establishment of the colony.
Good breeder: Usually available to ship within 1 to 3 months.
Slow breeder: Usually available to ship within 3 to 6 months.
Cryopreserved embryos 2 vials.
Approximate quantities: 20-30 embryos/vial.
Usually available to ship within 1 month.
Cryopreserved sperm 2 straws. Usually available to ship within 1 month.
Recovered litters from cryopreserved embryos Limited quantities of breeder mice (recovered litter) are available from a cryoarchive.
You will receive the resulting litter.
The actual number of animals, the gender and genotype ratios will vary.
Usually available to ship in 2 to4 months.
Recovered litters from cryopreserved sperm Limited quantities of breeder mice (recovered litter) are available from a cryoarchive.
You will receive the resulting litter.
The actual number of animals, the gender and genotype ratios will vary.
Usually available to ship in 2 to 4 months.
Chimeric mice from blastocyst injection of ES cell clone Cryopreserved cell Please contact our Cell Bank by E-mail ( cellqa@brc.riken.jp ) . more than 3 months

*: Please note that it may take more than 6 months to make arrangements to distribute.

 

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2. Fee

Our distribution fee is to partially recover production and ship costs and additional fee may be charged for any special handling or services. International shipping charges are payable on delivery.

Fees to domestic users include consumption tax.
The following fees are correct as of April 1, 2014 Price is subject to change without notice.

Fee (JPY)
For use in research for not-for-profit
academic purpose
 (*5)
For use in research for-profit-research
purpose
 (*6)
Live mice (per animal) (*1)(*4) ¥12,400 ¥24,800
Cryopreserved embryos Cryopreserved embryos (2 tubes) ¥24,200 ¥48,400
Recovered litters from cryopreserved
embryos (*1)
¥67,300 ¥134,600
Cryopreserved sperm Cryopreserved sperm (2 straws) ¥10,400 ¥20,800
Recovered litters from cryopreserved sperm
(*1)
¥106,000 ¥212,000
Recovery of
cryopreserved strains
from the FIMRe members
(*2)
Recovered litters from cryopreserved embryos ¥43,100 ¥86,200
Recovered litters from cryopreserved sperm ¥95,500 ¥191,000
Chimeric mice from blastocyst injection of
ES cell clone
¥333,300 ¥666,600
Mouse DNA (ten micro gram) (*3) ¥3,100 ¥6,200
Additional microbe tests Culture (per test) ¥1,900 ¥1,900
Serology (per test) ¥2,900 ¥2,900
PCR (per test) ¥4,320 ¥4,320
Microscopy (per test) ¥1,900 ¥1,900
RIKEN BRC standard test (per test) ¥34,200 ¥34,200
  • Fees do not include the cost of shipping containers and freight, which will be separately charged.
  • (*1) RIKEN BRC conducts routine microbe tests of animal facilities using test mice. If you request additional tests, extra fees are required.
  • (*2) The cost of shipping containers and freight will be separately charged to a user. Microbe tests of foster mothers will be conducted and the cost of the tests will be also charged to the user. In addition, when you request a frozen resource from a FIMRe organization, you need to pay fees of the resource and shipping cost of dry shipper separately.
  • (*3) You can order Mouse DNA from either the Experimental Animal Division or the Gene Engineering Division . Shipments will be made by the Gene Engineering Division.
  • (*4) If you wish to request organs or tissues, please contact the Experimental Animal Division (animal@brc.riken.jp ).
  • (*5) This refers to the use of our bioresource in research for not-for-profit academic purpose by a not-profit organization. Category I MTA is needed.
  • (*6) This refers to the use of our bioresource in research for the following cases. Category II MTA is needed.
    • For research to be conducted by for-profit organization
    • For collaborative research between for-profit organization and not-for-profit organization
    • For research by not-for-profit organization outsourced and sponsored by for-profit organization
    • For for-profit research by not-for-profit organization including R&D with the aim of patent acquisition
  • For Mouse resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc., a license fee of JPY 8,250 is separately required per strain.
  • For further information, please contact the Experimental Animal Division animal@brc.riken.jp ).

Estimate_e

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3. How to order

The mouse strains available from the RIKEN BRC are listed on the website and catalog. Users are required to complete the Order Form ((Credit Card Payment) or (Bank transfer/check [PDF], [Word])) and Material Transfer Agreement for Distribution (Form C 1 ( Sample ) for not-profit or Form C_P 2 ( Sample ) for profit) and send these documents to the following address.

Experimental Animal Division
RIKEN BioResource Center
3-1-1 Koyadai, Tsukuba
Ibaraki 305-0074, Japan

Fax: +81-29-836-9010

Please use an appropriate category of Material Transfer Agreement (MTA).
Category I MTA
For not-for-profit academic research by not-for-profit organization.
Category II MTA
Applicable for the following use:

a. For research to be conducted by for-profit organization
b. For collaborative research between not-for-profit organization and for-profit organization
c. For contract research by not-for-profit organization outsourced and sponsored by for-profit organization
d. For for-profit research by not-for-profit organization, including R&D with the aim of patent aquisition

In addition to Category I or II MTA, certain resources require prior written permission of the depositors.

Some Biological Resources require an Approval Form ( Form D ) from the Depositor. For such mice, please fill in Form D and send it to the Depositor to obtain his or her permission. Upon receiving from the Depositor, submit the form to us together with Form B and Form C(Sample) or Form C_P(Sample). Depositors can be contacted at the addresses listed on the website and catalog of the RIKEN BRC. If you cannot find their name and address on the website or catalog, or if you have any questions, please contact us by e-mail or by fax.

RIKEN BRC signs GFP Transfer License with GE Healthcare Bio-Sciences 

The signing of a license agreement with TET Systems

Clontech Laboratories, Inc. and RIKEN BioResource Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

 

Form B ORDER FORM (Credit Card Payment)
Form B ORDER FORM (Bank transfer or check payment)  form_b
Form C MATERIAL TRANSFER AGREEMENT(FOR DISTRIBUTION)form c(PDF) form c(Word)  (Sampleform c(PDF))
Form C_P MATERIAL TRANSFER AGREEMENT(FOR DISTRIBUTION) 書式4(PDF) 書式4(Word) (Sampleform c(PDF))
Form D APPROVAL FORM form d(PDF) form d(Word)
Form G ACCEPTANCE OF RESPONSIBILITY FOR LIVING MODIFIED ORGANISM form g(PDF) form g(Word)
GFP license of GE Healthcare Schedule A Schedule A(For Non-profit)(Sample書式5(PDF))
GFP license of GE Healthcare GFP license of GE Healthcare(For profit)
FP license of Clontech MATERIAL TRANSFER AGREEMENT(FOR DISTRIBUTION)MTA(For Non-profit)

 

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4. Terms of use

Please read the terms below. You are deemed to accept them when placing your order.

1) Possible cases for compensation and report deadline

Cases for compensation

Report deadline (after the shipping date)

①    Mice undelivered, mice deceased or moribund on arrival, frozen embryos or sperm thawed on arrival, damaged container, etc.

within 14 days

②    Failure to re-derive live mice from frozen embryos or sperm, genetic contamination, etc.

within 3 months

③   Unable to confirm the properties of the mouse strain described in the catalog, etc.

within a year

If you experience such a case as stated above, please send your written notification or email to the Experimental Animal Division ( animal@brc.riken.jp ) by each deadline.

We will take necessary action immediately.

2) Conditions and limitations for compensation

If you report us a case of ①, ② or ③ before the designated deadline, we will replace the resource you order with the same or alternative strain one time only free of charge, issue credit (the right to request the same or an equivalent strain whenever necessary in future, if no strain is needed at the moment) or refund. Refund will not exceed the distribution fee. Shipping cost for replacement is to be covered by the user.
There is no compensation for non-productive mice, mice younger than four-week old that arrive deceased or moribund, and pregnant mice that do not give birth. In the last two cases, resources are shipped at the user’s request. We do not compensate in case of failure or damage caused by the users.

3) Cancellation fee

Upon receiving order from a user, we will start preparing the requested resource by required processes including re-deriving the mice from frozen embryos or sperm and/or propagating a stock. These preparations are very costly. When a user cancel the order as a matter of user’s convenience, the user will be charged for the costs we spend on preparation up until cancellation is notified (up to 100% of distribution fee). When cancellation is notified after the BRC sends out the resource, 100% of distribution fee plus shipping cost will be charged to the user.

4) Accident during shipment

Our Center is not liable for any accident that occurs during shipping. Please select a carrier and make necessary arrangements on your own responsibility.
For overseas shipment, the users are responsible for customs clearance including preparation of necessary documents.

5) Information release on resources with quality defect

Whenever we note a quality defect in our resource, we will announce it through our website. We will also individually contact the users who received such a resource in the last 5 years.

 

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5. Payment

International Orders 
When you order the mice, please choose one of the following methods of payment listed below and send us your method of payment. We will send you an invoice and an appropriate instruction for each method as an attachment in PDF.
* Please note that the method of payment cannot be changed after we start processing to issue the invoice.

1) Credit card (MasterCard or VISA only)
Click here for further details.

2) Bank transfer
Remit the charge to the following account. Any bank fees are to be covered by the payer.

BeneficiaryRIKEN (The Institute of Physical and Chemical Research)
BankThe Bank of Tokyo-Mitsubishi UFJ, Ltd.
Swift addressBOTKJPJT
BranchWakoekimae Branch (Branch No.727)
Account No.159228 (Ordinary Account)
Bank Address1-10-20 Maruyamadai, Wako-shi, Saitama, 351-0112 Japan
Bank Tel+81-48-466-3611

3) Check
Mail us a bank check in Japanese yen, payable to ” The Institute of Physical and Chemical Research”, along with a copy of the invoice.

Please mail the bank check to:

Tsukuba Planning Section
Research Promotion Division
RIKEN Tsukuba Campus
3-1-1 Koyadai, Tsukuba
Ibaraki 305-0074, Japan
Fax: +81-29-836-9182

Domestic Orders 
Remit the charge to the account indicated below. Any bank fees are to be covered by the payer.

Beneficiary The Institute of Physical and Chemical Research
BankThe Bank of Joyo, Ltd.
BranchYatabe Branch (Branch No. 040)
Account No.6229211 (Ordinary Account)
Bank Address6006-1 Aza Fujizuka
Tsukuba-shi, Ibaraki
305-0861 Japan

<< *Please note!>> 
RIKEN is a non-profit organization and therefore requires all applicants to cover all bank charges, (including exchange, commission and handling fees) when sending payments for resources.

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6. Request for publication

We hope to share data obtained with the biological resources from RIKEN BRC.
Therefore, please send us a reprint of any publication of which results arise from the use of our mice.

BRC mice in Publications

Research Resource Circulation

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