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Video protocol

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

JoVE 3155 11/18/2011

Health Report

Sentinel mouse program
The RIKEN BRC has adopted the sentinel mouse program, a specialized approach with the microisolator cage system. A sentinel mouse cage containing five mice is laid on a microisolator cage rack and small pieces of bedding material from all cages of the rack are collected and placed in the sentinel mouse cage. Two mice from a sentinel cage are subjected to examination quarterly; one is for in-house examination and the other is sent to the ICLAS monitoring center of the Central Institute for Laboratory Animals, Kawasaki, Japan. The health report you receive is a copy of the then-most-recent Health Report guaranteed by a veterinarian at the ICLAS Monitoring Center.
We classify the microorganisms into four classes according to the risk presented to the mouse in Japan. All sentinel mice are tested for class A and B. Sentinel mice kept in the room for immuno-deficient mice are screened for class C as well. Class D contains certain pathogens believed not to exist in the Japanese mouse colony; therefore, mice are examined only at the request and expense of the recipient.

Announcement on monitoring of Hantaan virus and dermatophytes

Health Report  pdficon_s (BRC facility Health Report in the last year and a half)

Health Reports in RIKEN BRC Cryorecovery Work pdficon_s

Microbiological monitoring


Bimonthly Sentinel Health Report

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