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BRC facility Health Report
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IMPC 遺伝子・表現型 検索

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ビデオプロトコル

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

JoVE 3155 11/18/2011

Frozen Sperm Data


Protocol  < Japanese  / English >

Recovery test results

  Spermatozoa No of oocytesMedia
BRC_NoStrain_nameConditionGenotypeStrain of femalesFertilizationTested(%)SpermInsemination
00067C.Cg-Tnc/Rbrcfrozen sperm-/-BALB/cAJcl939895MβCD + PVA-HTFGSH + HTF
00208M. spretusfrozen sperm-same2454MβCD + PVA-HTFGSH + HTF
00209MSM/Msfrozen sperm-same6726625MβCD + PVA-HTFGSH + HTF
00213PWKfrozen sperm-same374582MβCD + PVA-HTFGSH + HTF
00302B6.129S6-Rela/3Rbrcfrozen sperm+/-C57BL/6JJcl6612852HTFHTF
00306TNF.K.Ofrozen sperm-/-C57BL/6JJcl17419191MβCD + PVA-HTFHTF
00349B6D2-Tg(CAG-AR*97Q)2-6Sobufrozen spermTg/+Jcl:B6D2F113134438HTFHTF
00438B6.129P2-Map2k4/Rbrcfrozen sperm+/-C57BL/6JJcl115919HTFHTF
00443DAN transgenic micefrozen spermTg/+Crlj:B6D2F124844955HTFHTF
00639JF1/Msffrozen sperm-same899297MβCD + PVA-HTFGSH + HTF
00654SWN/Msfrozen sperm-same105219MβCD + PVA-HTFGSH + HTF
00667PGN2/Msfrozen sperm-same4917029MβCD + PVA-HTFGSH + HTF
00770TgN(tw5BAC)4-1Imegfrozen spermTg/+C57BL/6JJcl3416720HTFHTF
00771C57BL/6J-Tg(GOFGFP)11Imegfrozen spermTg/+C57BL/6JJcl35466154HTFHTF
00784C57BL/6-Tg(GOFMBD)1Imegfrozen spermTg/+C57BL/6JJcl40658869HTFHTF
00787C57BL/6-Tg(GOFMBD)5Imegfrozen spermTg/?C57BL/6JJcl223465HTFHTF
00788C57BL/6-Tg(deltaMBD)1Imegfrozen spermTg/+C57BL/6JJcl8716453HTFHTF
00821C57BL/6J-Tg(deGFP)18Imegfrozen spermTg/+C57BL/6JJcl2314016HTFHTF
00857trehalase Knockout micefrozen sperm-/-C57BL/6JJcl7513058MβCD + PVA-HTFGSH + HTF
00886B6;B6C3-Tg(Acro3-EGFP)01Osbfrozen spermTg/TgC57BL/6JJcl8212566HTFHTF
00895AID-Tg line #B2frozen spermTg/+C57BL/6NCrSlc13718873HTFHTF
00896C57BL/6-Tg(CAG-Aicda,CAG-EGFP)C1Hon/HonRbrcfrozen spermTg/+C57BL/6NCrSlc19232359HTFHTF
00904C.129S2-Pdcd1/HonRbrc (N12)frozen sperm-/-BALB/cCrSlc111861MβCD + PVA-HTFGSH + HTF
00998C57BL/6-TgN(muAqp0)4Tohmfrozen spermTg/?C57BL/6NCrSlc24044754HTFHTF
01000Fyn Targeting micefrozen sperm-/-C57BL/6NCrlCrlj1716410HTFHTF
01080B6.129S-Drd1afrozen sperm-/-C57BL/6JJcl9815663MβCD + PVA-HTFGSH + HTF
01099ERAI micefrozen spermTg/+C57BL/6JJcl9013865MβCD + PVA-HTFGSH + HTF
01142ICR.Cg-Tg(CAG-cat,-Notch1)1Ysa/YsaRbrcfrozen spermTg/+Jcl:MCH17928463HTFHTF
01143CAG-CAT-Delta1frozen spermTg/+Jcl:MCH5216831MβCD + PVA-HTFHTF
01151hairy and enhancer of split-related 1frozen sperm-/-C57BL/6NJcl16226461HTFHTF
01340Okh (grayish white)frozen spermm/mOkh (grayish white)264854MβCD + PVA-HTFGSH + HTF
01345B6.129P2-Emx1/ItoRbrcfrozen spermKI/KIC57BL/6JJcl30040674MβCD + PVA-HTFGSH + HTF
01351B6.129S-Grm2/NakRbrcfrozen sperm-/-C57BL/6NCrlCrlj3011925HTFHTF
01372CAG-CAT-Dll4#1frozen spermTg/+Jcl:MCH9618951MβCD + PVA-HTFHTF
01413C.B6D2-Tg(CAG-GFP/LC3)53Nmz/Rbrcfrozen spermTg/+BALB/cAnNCrlCrlj283385MβCD + PVA-HTFGSH + HTF
01414C3.B6D2-Tg(CAG-GFP/LC3)53Nmz/Rbrcfrozen spermTg/TgC3H/HeNCrlCrlj15617191MβCD + PVA-HTFGSH + HTF
01418C3.HR1-Hr
/Rbrc
frozen spermhr/hrC3H/HeNCrlCrlj20621397MβCD + PVA-HTFGSH + HTF
01492B6.Cg-Tg(DBH-cre)9-9Koba/KobaRbrcfrozen spermTg/+C57BL/6JJcl8613265MβCD + PVA-HTFGSH + HTF
01578B6.Cg-Pik3r1/Rbrcfrozen sperm+/-C57BL/6NCrlCrlj5517432HTFHTF
01680PV384frozen spermGt/+C57BL/6JJcl11612791MβCD + PVA-HTFHTF
01726B6;129-Mfge8/OsaRbrcfrozen sperm-/-C57BL/6NCrlCrlj4525917MβCD + PVA-HTFHTF
01737B6;129S4-Dnmt1 frozen sperm+/-C57BL/6JJcl21825287MβCD + PVA-HTFGSH + HTF
01840GluRε4 KO mousefrozen sperm+/-C57BL/6NJcl619664HTFHTF
02096B6.Cg-Tg(CAG-floxed Neo-EGFP)REP08Osbfrozen spermTg/+C57BL/6NCrSlc10617361MβCD + PVA-HTFGSH + HTF
02190C.129P2-Nfe2l2frozen sperm-/-BALB/cAJcl13628847MβCD + PVA-HTFGSH + HTF
02237C57BL/6Cr-Tg(Dmp1-HBEGF)frozen spermTg/+C57BL/6NCrSlc28936180MβCD + PVA-HTFGSH + HTF
02259B6.129S-Krasfrozen sperm+/-C57BL/6JJcl19022883MβCD + PVA-HTFGSH + HTF
02271B6.Cg-Otx2/SiaRbrcfrozen spermflox/+C57BL/6N(Crl)8529129MβCD + PVA-HTFGSH + HTF
02306B6.Cg-Prkcbp1frozen sperm+/-C57BL/6JJcl152057HTFHTF
02322B6.129P2-CmahFrozen sperm-/-C57BL/6JJcl678183MβCD + PVA-HTFGSH + HTF
02339B6.Cg-Tg(Tec-p210 BCR/ABL1)1Homyfrozen spermTg/+C57BL/6NCrlCrlj929993MβCD + PVA-HTF GSH+ HTF
02368B6.Cg-Nr1i2frozen spermKI, fneo/KI, fneoBALB/cCrSlc16922675MβCD + PVA-HTFGSH + HTF
02370B6;129-Grnfrozen sperm-/-C57BL/6JJmsSlc81575MβCD + PVA-HTFGSH + HTF
02393C.Cg-Aicda(N12)frozen sperm-/-BALB/cCrSlc19829168MβCD + PVA-HTFGSH + HTF
02781B6.Cg-Cd8afrozen spermCd8a: a/a; Tg: Tg/+same617482MβCD + PVA-HTFGSH + HTF
02829B6.B6C3-Tg(Th-SNCA*130m)1702Yoshfrozen spermTg/+C57BL/6JJcl17855532MβCD + PVA-HTFGSH + HTF
02933C57BL/6-Tg(Olfr16-Olr226*D126R/R127D,-EYFP)1Hsakfrozen spermTg/+C57BL/6JCrlCrlj222698HTFHTF
03390B6;129-Apoe/3SfuRbrcfrozen spermKI/+C57BL/6NJcl20728174MβCD + PVA-HTFGSH + HTF
03418ApoE4 KOfrozen spermKI/+C57BL/6NJcl17218096MβCD + PVA-HTFGSH + HTF
04187B6.B6CB-Adam22frozen sperm+/-C57BL/6NCrlCrlj7119836MβCD + PVA-HTFGSH + HTF
04200B6.129P2-Cxcl12frozen spermKI/+C57BL/6JJcl41559270MβCD + PVA-HTFGSH + HTF
04502B6.Cg-Pbwg1/19Ngafrozen spermcastaneus/castaneusC57BL/6JJcl13043130MβCD + PVA-HTFGSH + HTF
04550B6.129S2-Nmsfrozen sperm+/-C57BL/6JJcl14964623MβCD + PVA-HTFGSH + HTF
04553B6.Cg-Nml/2frozen sperm+/-C57BL/6JJcl16520381MβCD + PVA-HTFGSH + HTF
04609B6;129P2-Wnt5afrozen spermflox/+C57BL/6NCrlCrlj9821945MβCD + PVA-HTFGSH + HTF
04708FVB.Cg-Tg(Slc6a5-EGFP)13Uzefrozen spermTg/+FVB/NJcl25425998MβCD + PVA-HTFGSH + HTF
04791B6.129P2-Dapk1frozen sperm+/-C57BL/6JJcl13126949MβCD + PVA-HTFGSH + HTF
04880B6.129-Gp2frozen sperm+/-C57BL/6N(Crl)384781MβCD + PVA-HTFGSH + HTF
04902B6;129X1-Ifngfrozen sperm-/-C57BL/6JJmsSlc314597MβCD + PVA-HTFGSH + HTF
04904B6;129P2-Cxcr4frozen spermflox/+C57BL/6JJmsSlc19023681MβCD + PVA-HTFGSH + HTF
04916B6.129P2-Gnb1frozen spermGt/+C57BL/6JJmsSlc8527431MβCD + PVA-HTFGSH + HTF
04918B6;129S2-Il6frozen sperm-/-C57BL/6JJmsSlc3510832MβCD + PVA-HTFGSH + HTF
04945C.129S4(B6)-Il5frozen spermKI/KIBALB/cCrSlc384388MβCD + PVA-HTFGSH + HTF
04948B6.B6CB(Cg)-Psmg1frozen spermflox/floxC57BL/6JJmsSlc11213086MβCD + PVA-HTFGSH + HTF
04949B6.B6CB(Cg)-Psmd14frozen spermflox/+C57BL/6JJmsSlc10012282MβCD + PVA-HTFGSH + HTF
05073B6;B6D2-Tg(CAG-flag-H2A)frozen spermTg/+C57BL/6JJmsSlc3111726MβCD + PVA-HTFGSH + HTF
05118B6;B6CB-Tnffrozen sperm-/-C57BL/6JJmsSlc8813864MβCD + PVA-HTFGSH + HTF
05145B6.129P2-Prdm9frozen sperm+/-C57BL/6JJmsSlc9213966MβCD + PVA-HTFGSH + HTF
05233ICR;B6D2-Tg(ERE-Luc)4Tysnfrozen spermTg/+Slc:ICR10110596MβCD + PVA-HTFHTF
05234ICR;B6D2-Tg(ERE-Luc)5Tysnfrozen spermTg/+Slc:ICR10410698MβCD + PVA-HTFGSH + HTF
05271ICR-Tg(p16-luc)53Eiha/Rbrcfrozen spermTg/+Crlj:CD1(ICR)16118985MβCD + PVA-HTFGSH + HTF
05281
LtbR (Acc. No. CDB0531K)frozen sperm+/-C57BL/6NJcl12713793MβCD + PVA-HTFGSH + HTF
05282LMIR5 (Acc. No. CDB0539K)frozen sperm+/-C57BL/6NJcl14035739MβCD + PVA-HTFGSH + HTF
05289D2;B6-Tg(CAG-EGFP)C14-Y01-FM131Osbfrozen spermTg/+DBA/2CrSlc9211183MβCD + PVA-HTFGSH + HTF
05290B6.129P2-Dscam/A151Kzyfrozen sperm+/-C57BL/6NCrSlc9411780MβCD + PVA-HTFGSH + HTF
05382B6.Cg-Tg(Dppa3-ECFP)#Sait/Rbrcfrozen spermTg/+C57BL/6NJcl33986039MβCD + PVA-HTFGSH + HTF
05385Dppa3-MER-Cre-MER-PEST (Acc. No. CDB0475T)frozen spermTg/TgC57BL/6NCrSlc22753942MβCD + PVA-HTFGSH + HTF
05445B6;129-Htr5bfrozen spermKI/+C57BL/6JJcl15266023MβCD + PVA-HTFGSH + HTF
05450B6;CBB6(129)-Tg(Mlc1-tTA)2Rhnfrozen spermTg/+C57BL/6JJcl43575058MβCD + PVA-HTFGSH + HTF
05494B6;129P2-Tnfaip3frozen spermflox/+C57BL/6N(Crl)19320893MβCD + PVA-HTFGSH + HTF
05495B6;129P2-Tnfaip3frozen sperm+/-C57BL/6N(Crl)7911370MβCD + PVA-HTFGSH + HTF
05515B6;129S4-Ern1frozen spermflox/+C57BL/6JJcl21624389MβCD + PVA-HTFGSH + HTF
05517B6;129S4-Xbp1frozen sperm+/-C57BL/6JJcl13335537MβCD + PVA-HTFGSH + HTF
05523B6.129S7-Sox9frozen spermKI/+C57BL/6NCrSlc11119756MβCD + PVA-HTFGSH + HTF
05524B6.Cg-Tg(Col1a1-cre)1Haakfrozen spermTg/+C57BL/6NCrSlc16718988MβCD + PVA-HTFGSH + HTF
05539B6.129-Gt(ROSA)26Sorfrozen spermKI/KIC57BL/6JJcl18919995MβCD + PVA-HTFGSH + HTF
05564B6.Cg-Tg(Chx10-cre)G5TfurFrozen spermTg/+C57BL/6NCrlCrl8011371MβCD + PVA-HTFGSH + HTF
05582C57BL/6N-Ikzf3/CB3frozen sperm+/-C57BL/6NTac16458028MβCD + PVA-HTFGSH + HTF
05589C57BL/6N-Trpc5/B02frozen spermflox/YC57BL/6NTac22649646MβCD + PVA-HTFGSH + HTF
05637B6D2;129S2-Igsf8frozen spermflox/+Jcl:B6D2F125828192MβCD + PVA-HTFGSH + HTF
05638B6;129-Novfrozen sperm-/-C57BL/6JJcl12049624MβCD + PVA-HTFGSH + HTF
05649B6.129S4-Plin5/Rbrcfrozen sperm+/-C57BL/6JJcl35865155MβCD + PVA-HTFGSH + HTF
05654B6.Cg-Tg(CAG-mRFP1,-SOX9,EGFP)1Haakfrozen spermTg/+C57BL/6NCrSlc21456438MβCD + PVA-HTFGSH + HTF
05658B6.Cg-Siglechfrozen spermKI/KIC57BL/6J(Crl)25879832MβCD + PVA-HTFGSH + HTF
05659B6.Cg-Ly75frozen spermKI/KIC57BL/6J(Crl)15563724MβCD + PVA-HTFGSH + HTF
05685C57BL/6N-Oxtr/AC7frozen sperm+/-C57BL/6NTac12423752MβCD + PVA-HTFGSH + HTF
05690C57BL/6N-Sst/F04frozen spermtm1a/+C57BL/6NTac11527642MβCD + PVA-HTFGSH + HTF
05702C57BL/6J-Tg(CAG-XBP1/luc)23Tiwfrozen spermTg/+C57BL/6JJcl14860524MβCD + PVA-HTFGSH + HTF
05737B6.Cg-Tg(CAG-tdKaede)15Utrfrozen spermTg/?C57BL/6JJcl21185325MβCD + PVA-HTFGSH + HTF
05744B6.Cg-Tg(Grm6-cre)7Tfurfrozen spermTg/+C57BL/6NCrlCrlj19520595MβCD + PVA-HTFGSH + HTF
05769B6(129S6)-Tg(Aif1-tTA)54Kftnkfrozen spermTg/+C57BL/6JJcl4515329MβCD + PVA-HTFGSH + HTF
05770B6.Cg-Tg(Pvalb-tTA)1Kftnkfrozen spermTg/+C57BL/6JJcl47363674MβCD + PVA-HTFGSH + HTF
05808B6.129P2-Aldh2frozen sperm-/-C57BL/6JJcl32538185MβCD + PVA-HTFGSH + HTF
05842C57BL/6-Tg(tetO-ArchT/EGFP)1Ahkyfrozen spermTg/+C57BL/6JJcl30142770MβCD + PVA-HTFGSH + HTF
05846B6.Cg-Tg(Tph2-tTA)1Ahkyfrozen spermTg/TgC57BL/6JJcl18936851MβCD + PVA-HTFGSH + HTF
05850B6.Cg-Flgfrozen sperm-/-C57BL/6JJcl217184712MβCD + PVA-HTFGSH + HTF
05879B6;129P2-Kcnj11frozen sperm-/-C57BL/6JJcl28248658MβCD + PVA-HTFGSH + HTF
05892B6.129P2-Reck/Nodafrozen sperm+/-C57BL/6JJmsSlc20921398MβCD + PVA-HTFGSH + HTF
05893B6.Cg-Tg(AKT/Ert2,EGFP)1Nknfrozen spermTg/?C57BL/6NCrSlc485292MβCD + PVA-HTFGSH + HTF
05911B6.129S6-Cd109frozen sperm+/-C57BL/6JJmsSlc8319044MβCD + PVA-HTFGSH + HTF
05912C57BL/6N-Tg(CAG-L-Adipo)1Shbfrozen spermTg/+C57BL/6NCrSlc11011596MβCD + PVA-HTFGSH + HTF
05918B6.Cg-Tg(TOPGAL)frozen spermTg/+C57BL/6JJcl28176737MβCD + PVA-HTFGSH + HTF
05924B6.Cg-Atpif1frozen sperm-/-C57BL/6JJmsSlc15533646MβCD + PVA-HTFGSH + HTF
05950B6.Cg-Lrrc32frozen spermflox/floxC57BL/6JJcl556980HTFHTF
06037C57BL/6-Pou5f1frozen spermKI/+C57BL/6NJcl28236477MβCD + PVA-HTFGSH + HTF
06302NEAT1(lacZ)(Acc.No.CDB0773K)frozen sperm+/-C57BL/6NCrSlc28733586MβCD + PVA-HTFGSH + HTF
06313B6.129-Sparcfrozen sperm-/-C57BL/6JJcl438113039MβCD + PVA-HTFGSH + HTF
06323B6.Cg-Ednrafrozen spermflox/floxC57BL/6J(Crl)9421344MβCD + PVA-HTFGSH + HTF
06332C57BL/6N-Nin/B10frozen spermtm1b/+C57BL/6N(Crl)11813190MβCD + PVA-HTFGSH + HTF
06339ICR.Cg-Tg(Olfr1507-cre)1Hsakfrozen spermTg/+Crlj:CD1(ICR)5914541MβCD + PVA-HTFGSH + HTF
06446B6.129-Clmpfrozen sperm+/-C57BL/6JJcl9714766MβCD + PVA-HTFGSH + HTF
06447ICR.129-Clmpfrozen sperm+/-Jcl:ICR438352MβCD + PVA-HTFGSH + HTF
06462C57BL/6-Prkcdfrozen spermflox/floxC57BL/6N(Crl)32962752MβCD + PVA-HTFGSH + HTF
06463C57BL/6-Prkcdfrozen sperm+/-C57BL/6N(Crl)39743392MβCD + PVA-HTFGSH + HTF
06509ICR.Cg-Tg(Oct4dPE-creERT2)4frozen spermTg/+Jcl:MCH (ICR)8428729MβCD + PVA-HTFGSH + HTF
06516B6.Cg-Tdrd7frozen sperm+/-C57BL/6JJcl459113041MβCD + PVA-HTFGSH + HTF
09256B6.Cg-Gt(ROSA)26Sorfrozen spermKI/KIC57BL/6NJcl120117610MβCD + PVA-HTFGSH + HTF
09295B6.Cg-Unc5dfrozen sperm+/-C57BL/6JJcl17944440MβCD + PVA-HTFGSH + HTF
09365STOCK Gt(ROSA)26SorIgs7/Jfrozen spermGt(ROSA): KI/+; Igs7: KI/+C57BL/6JJcl747895MβCD + PVA-HTFGSH + HTF
09453FVB-Tg(Thy1-GCaMP6, -mCherry)1Shifrozen spermTg/TgFVB/NJcl36947378MβCD + PVA-HTFGSH + HTF
09483B6.B6CB-Rnf31frozen spermflox/floxC57BL/6NCrSlc26736573MβCD + PVA-HTFGSH + HTF
09486B6.Cg-Xcr1frozen spermKI/+C57BL/6JJcl11916273MβCD + PVA-HTFGSH + HTF
09508C57BL/6J-Tg(RP11-94J18/RP11-662G11)BMyfrozen spermTg/+C57BL/6JJcl26964442MβCD + PVA-HTFGSH + HTF
09545STOCK-Tg(Slc6a3-tTA)15Kftnkfrozen spermTg/?C57BL/6JJcl13113498MβCD + PVA-HTFGSH + HTF
09566B6.Cg-Ickfrozen spermflox/+C57BL/6JJcl697888MβCD + PVA-HTFGSH + HTF
09595B6.129P2-Keap1frozen spermflox/floxC57BL/6JJmsSlc32738585MβCD + PVA-HTFGSH + HTF
09599FVB.129S6-Cd109frozen sperm-/-FVB/NJcl21954240MβCD + PVA-HTFGSH + HTF
GSC0092M100514frozen spermm/+C57BL/6JJcl73477994MβCD + PVA-HTFGSH + HTF
KIT0022KITOX-IDM33-AEfrozen spermM/+C57BL/6JJcl7118938HTFHTF