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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

JoVE 3155 11/18/2011

Frozen Sperm Data


Protocol  < Japanese  / English >

Recovery test results  (PDF version)

Recovery test results

BRC_No Strain_name Spermatozoa Strain of females No of oocytes Media
Condition Genotype Fertilization Tested (%) Sperm Insemination
00302 RKO-3 frozen sperm +/- C57BL/6JJcl 66 128 52 HTF HTF
00306 TNF.K.O frozen sperm -/- C57BL/6JJcl 174 191 91 MβCD + HTF HTF
frozen sperm -/- C57BL/6JJcl 78 91 86 HTF HTF
00349 B6D2-Tg(CAG-AR*97Q)2-6Sobu frozen sperm Tg/+ Jcl:B6D2F1 131 344 38 HTF HTF
00438 B6.129P2-Map2k4<tm1Pngr>/Rbrc frozen sperm +/- C57BL/6JJcl 11 59 19 HTF HTF
frozen sperm +/- C57BL/6JJcl 97 237 41 MβCD + HTF HTF
00443 DAN transgenic mice frozen sperm Tg/+ Crlj:B6D2F1 248 449 55 HTF HTF
00770 TgN(tw5BAC)4-1Imeg frozen sperm Tg/+ C57BL/6JJcl 34 167 20 HTF HTF
00771 C57BL/6J-Tg(GOFGFP)11Imeg frozen sperm Tg/+ C57BL/6JJcl 354 661 54 HTF HTF
00784 C57BL/6-Tg(GOFMBD)1Imeg frozen sperm Tg/+ C57BL/6JJcl 406 588 69 HTF HTF
00788 C57BL/6-Tg(deltaMBD)1Imeg frozen sperm Tg/+ C57BL/6JJcl 87 164 53 HTF HTF
00821 C57BL/6J-Tg(deGFP)18Imeg frozen sperm Tg/+ C57BL/6JJcl 23 140 16 HTF HTF
frozen sperm Tg/Tg C57BL/6JJcl 3 45 7 HTF HTF
00886 B6;B6C3-Tg(Acro3-EGFP)01Osb frozen sperm Tg/Tg C57BL/6JJcl 82 125 66 HTF HTF
00895 AID-Tg line #B2 frozen sperm Tg/+ C57BL/6NCrSlc 137 188 73 HTF HTF
00896 AID-Tg line #C1 frozen sperm Tg/+ C57BL/6NCrSlc 192 323 59 HTF HTF
00998 C57BL/6-TgN(muAqp0)4Tohm frozen sperm Tg/? C57BL/6NCrSlc 240 447 54 HTF HTF
01000 Fyn Targeting mice frozen sperm -/- C57BL/6NCrlCrlj 17 164 10 HTF HTF
01080 B6.129S-Drd1a<tm1Mok> frozen sperm -/- C57BL/6JJcl 98 156 63 MβCD + HTF GSH + HTF
01099 ERAI mice frozen sperm Tg/+ C57BL/6JJcl 90 138 65 MβCD + HTF GSH + HTF
01142 CAG-CAT-Notch1 frozen sperm Tg/+ Jcl:MCH 179 284 63 HTF HTF
frozen sperm Tg/+ Jcl:MCH 111 149 74 MβCD + HTF HTF
01143 CAG-CAT-Delta1 frozen sperm Tg/+ Jcl:MCH 52 168 31 MβCD + HTF HTF
01151 hairy and enhancer of split-related 1 frozen sperm -/- C57BL/6NJcl 162 264 61 HTF HTF
01345 Emx1-Cre KI Δneo frozen sperm KI/KI C57BL/6JJcl 300 406 74 MβCD + HTF GSH + HTF
01351 B6.129S-Grm2<tm1Nak>/NakRbrc frozen sperm -/- C57BL/6NCrlCrlj 30 119 25 HTF HTF
frozen sperm -/- C57BL/6NCrlCrlj 36 162 22 MβCD + HTF HTF
01372 CAG-CAT-Dll4#1 frozen sperm Tg/+ Jcl:MCH 96 189 51 MβCD + HTF HTF
01413 C.B6D2-Tg(CAG-GFP/LC3)53Nmz/Rbrc frozen sperm Tg/+ BALB/cAnNCrlCrlj 28 33 85 MβCD + HTF GSH + HTF
frozen sperm Tg/Tg BALB/cAnNCrlCrlj 10 29 34 MβCD + HTF GSH + HTF
01414 C3.B6D2-Tg(CAG-GFP/LC3)53Nmz/Rbrc frozen sperm Tg/Tg C3H/HeNCrlCrlj 156 171 91 MβCD + HTF GSH + HTF
01418 C3.HR1-Hr<hr>/Rbrc frozen sperm hr/hr C3H/HeNCrlCrlj 206 213 97 MβCD + HTF GSH + HTF
01578 C57BL/6-PI3K KO frozen sperm +/- C57BL/6NCrlCrlj 55 174 32 HTF HTF
01680 PV384 frozen sperm Gt/+ C57BL/6JJcl 116 127 91 MβCD + HTF HTF
01726 MFG-E8 Knockout mouse frozen sperm -/- C57BL/6NCrlCrlj 45 259 17 MβCD + HTF HTF
01840 GluRε4 KO mouse frozen sperm +/- C57BL/6NJcl 61 96 64 HTF HTF
frozen sperm +/- C57BL/6NJcl 113 156 72 MβCD + HTF HTF
02096 B6.Cg-Tg(CAG-floxed Neo-EGFP)REP08Osb frozen sperm Tg/+ C57BL/6NCrSlc 106 173 61 MβCD + HTF GSH + HTF
02306 B6.Cg-Prkcbp1<tm1.1Jtak> frozen sperm +/- C57BL/6JJcl 15 205 7 HTF HTF
02322 B6.129P2-Cmah<tm1Ykoz> Frozen sperm -/- C57BL/6JJcl 67 81 83 MβCD + HTF GSH + HTF
02339 B6.Cg-Tg(Tec-p210 BCR/ABL1)1Homy frozen sperm Tg/+ C57BL/6NCrlCrlj 92 99 93 MβCD + HTF  GSH+ HTF
02933 C57BL/6-Tg(Olfr16-Olr226*D126R/R127D,-EYFP)1Hsak frozen sperm Tg/+ C57BL/6JCrlCrlj 22 269 8 HTF HTF
04187 B6.B6CB-Adam22<tm1Sag> frozen sperm +/- C57BL/6NCrlCrlj 71 198 36 MβCD + HTF GSH + HTF
04609 B6;129P2-Wnt5a<tm1.1Homy> frozen sperm flox/+ C57BL/6NCrlCrlj 98 219 45 MβCD + HTF GSH + HTF
04902 B6;129X1-Ifng<tm1Yiw> frozen sperm -/- C57BL/6JJmsSlc 31 459 7 MβCD + HTF GSH + HTF
04904 B6;129P2-Cxcr4<tm1Yiw> frozen sperm flox/+ C57BL/6JJmsSlc 190 236 81 MβCD + HTF GSH + HTF
04918 B6;129S2-Il6<tm1Kopf> frozen sperm -/- C57BL/6JJmsSlc 35 108 32 MβCD + HTF GSH + HTF
04945 C.129S4(B6)-Il5<tm1Ktk> frozen sperm KI/KI BALB/cCrSlc 38 43 88 MβCD + HTF GSH + HTF
04948 B6.B6CB(Cg)-Psmg1<tm1.1Smta> frozen sperm flox/flox C57BL/6JJmsSlc 112 130 86 MβCD + HTF GSH + HTF
04949 B6.B6CB(Cg)-Psmd14<tm1.1Smta> frozen sperm flox/+ C57BL/6JJmsSlc 100 122 82 MβCD + HTF GSH + HTF
05073 B6;B6D2-Tg(CAG-flag-H2A) frozen sperm Tg/+ C57BL/6JJmsSlc 31 117 26 MβCD + HTF GSH + HTF
05118 B6;B6CB-Tnf<tm1Sek> frozen sperm -/- C57BL/6JJmsSlc 88 138 64 MβCD + HTF GSH + HTF
05233 ICR;B6D2-Tg(ERE-Luc)4Tysn frozen sperm Tg/+ Slc:ICR 101 105 96 MβCD + HTF HTF
05539 B6.129-Gt(ROSA)26Sor<tm1(Stop-Bmi1,EGFP)> frozen sperm KI/KI C57BL/6JJcl 51 56 91 MβCD + HTF GSH + HTF
05564 B6.Cg-Tg(Chx10-cre)G5Tfur Frozen sperm Tg/+ C57BL/6NCrlCrl 80 113 71 MβCD + HTF GSH + HTF
KIT0022 KITOX-IDM33-AE frozen sperm M/+ C57BL/6JJcl 71 189 38 HTF HTF