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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

March 2014 Novel Rosa26 Cre-reporter knock-in C57BL/6N mice


mouse_of_month_201403

Novel ROSA26 Cre-reporter knock-in C57BL/6N mice

C57BL/6N-Gt(ROSA)26Sor<tm1(CAG-EGFP,tdsRed)Utr>/Rbrc (RBRC04874)

201403

Figure reproduced from ref. 2 by the courtesy of the authors and the publisher.

 

Cre recombinase is a 38-kDa enzyme derived from bacteriophage P1 that specifically recognizes 34-bp loxP sites. Cre is among the most widely used site-specific recombinases in genome engineering. A number of reporter mouse strains have been engineered specifically for identifying the derivatives of Cre-expressing cells and include ROSA26-reporter mice (R26R mice) [1]. Here, the ROSA26 locus was targeted to generate new reporter mice (R26GRR mice) in which floxed EGFP and tandem dsRed (tdsRed) were inserted downstream of the CAG promoter [2]. As shown in the figure, R26GRR mice show green fluorescence in the cell of a wide range of organs prior to Cre recombinase exposure and show red fluorescence in the Cre-recombined cells. tdsRed yields negligible contamination of the EGFP signal, resulting in ideal dual-color labeling with EGFP [3]. R26GRR mice are also a good source of EGFP-positive cells with a C57BL/6N genetic background as this strain was produced using embryonic stem cells derived from a C57BL/6N blastocyst [4]. These novel R26GRR mice are a useful Cre-reporter C57BL/6N strain following Cre-mediated recombination.

 

Depositor : RIKEN BioResource Center
References : [1] Soriano P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet.; 21(1):70-1, 1999.
[2] Hasegawa Y, Daitoku Y, Sekiguchi K, Tanimoto Y, Mizuno-Iijima S, Mizuno S, Kajiwara N, Ema M, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K. Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination. Exp Anim.; 62(4):295-304, 2013.
[3] Bevis BJ, Glick BS. Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nat Biotechnol.; 20(1):83-7, 2002.
[4] Tanimoto Y, Iijima S, Hasegawa Y, Suzuki Y, Daitoku Y, Mizuno S, Ishige T, Kudo T, Takahashi S, Kunita S, Sugiyama F, Yagami K. Embryonic stem cells derived from C57BL/6J and C57BL/6N mice. Comp Med.; 58(4):347-52, 2008.

 

March 2014
Contact: Shinya Ayabe, Ph.D.
Experimental Animal Division, RIKEN BioResource Center
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